#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3991652	B	Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry.	Homo sapiens	243	ALA3991601	subcellular format	Kuster lab chemical proteomics drug profiling
2.	ALA4118328	B	Inhibition of CRK7 in human SKCO1 cells at 1 uM after 4 hrs using biotin labeled DIKCSNILLNNSGQIK probe by mass-spectrometric analysis relative to control	Homo sapiens	3	ALA4118021	cell-based format	Scientific Literature
3.	ALA4151504	B	Inhibition of CDK12 in human SK-BR-3 cells assessed as reduction in RNA polymerase 2 Ser2 phosphorylation incubated for 4 hrs by	Homo sapiens	3	ALA4145674	cell-based format	Scientific Literature
4.	ALA4151509	B	Time dependent inhibition of human CDK12 incubated for 0 to 5 hrs using ULight-4E-BP1 (Thr37/Thr46) substrate by LANCE system based assay	Homo sapiens	2	ALA4145674	single protein format	Scientific Literature
5.	ALA4375312	B	Binding affinity to wild-type human partial length CDK12 (P697 to E1077 residues) expressed in mammalian expression system measured after 1 hr by kinomescan method	Homo sapiens	1	ALA4373706	assay format	Scientific Literature
6.	ALA4611865	B	Inhibition of CDK12 (unknown origin)	Homo sapiens	4	ALA4610077	single protein format	Scientific Literature
7.	ALA4612766	B	Binding affinity to CDK12 (unknown origin) assessed as change in molecular weight at 10 fold excess of protein measured after 1 hr by LC-MS/MS analysis	Homo sapiens	1	ALA4610118	single protein format	Scientific Literature
8.	ALA4612768	B	Binding affinity to CDK12 (1025 to 1041 residues) (unknown origin) assessed as Cys1039-adduct formation at 10 fold excess of protein measured after 1 hr by by capillary electrophoresis-MS/MS analysis	Homo sapiens	1	ALA4610118	single protein format	Scientific Literature
9.	ALA4612789	B	Inhibition of CDK12 C1039S mutant in human HAP1 cells assessed as reduction in RNA Polymerase 2 CTD phosphorylation at Ser2 residues at 40 to 200 nM measured after 6 hrs by Western blot analysis	Homo sapiens	1	ALA4610118	cell-based format	Scientific Literature
10.	ALA4612790	B	Inhibition of CDK12 C1039S mutant in human HAP1 cells assessed as reduction in RNA Polymerase 2 CTD phosphorylation at Ser5 residues at 40 to 200 nM measured after 6 hrs by Western blot analysis	Homo sapiens	1	ALA4610118	cell-based format	Scientific Literature
11.	ALA4612791	B	Inhibition of CDK12 C1039S mutant in human HAP1 cells assessed as reduction in RNA Polymerase 2 CTD phosphorylation at Ser7 residues at 40 to 200 nM measured after 6 hrs by Western blot analysis	Homo sapiens	1	ALA4610118	cell-based format	Scientific Literature
12.	ALA4612792	B	Inhibition of CDK12 C1039S mutant in human HAP1 cells assessed as reduction in RNA Polymerase 2 expression at 40 to 200 nM measured after 6 hrs by Western blot analysis	Homo sapiens	1	ALA4610118	cell-based format	Scientific Literature
13.	ALA4612805	B	Inhibition of CDK12 C1039S mutant in human HAP1 cells assessed as reduction in RAD51 expression at 40 to 200 nM measured after 24 hrs by Western blot analysis	Homo sapiens	1	ALA4610118	cell-based format	Scientific Literature
14.	ALA4612807	B	Inhibition of CDK12 C1039S mutant in human HAP1 cells assessed as reduction in RAD51C expression at 40 to 200 nM measured after 24 hrs by Western blot analysis	Homo sapiens	1	ALA4610118	cell-based format	Scientific Literature
15.	ALA4612809	B	Inhibition of CDK12 C1039S mutant in human HAP1 cells assessed as reduction in RAD51 RNA expression at 40 to 200 nM measured after 24 hrs by RT-qPCR analysis	Homo sapiens	1	ALA4610118	cell-based format	Scientific Literature
16.	ALA4612811	B	Inhibition of CDK12 C1039S mutant in human HAP1 cells assessed as reduction in RAD51C RNA expression at 40 to 200 nM measured after 24 hrs by RT-qPCR analysis	Homo sapiens	1	ALA4610118	cell-based format	Scientific Literature
17.	ALA4612813	B	Inhibition of CDK12 in human HAP1 cells assessed as potentiation of olaparib-induced antiproliferative activity at 25 nM measured after 8 days by Celltiter glo assay	Homo sapiens	1	ALA4610118	cell-based format	Scientific Literature
18.	ALA4613418	B	Inhibition of CDK12 (unknown origin)	Homo sapiens	1	ALA4613207	single protein format	Scientific Literature
19.	ALA4739798	B	Inhibition of biotinylated-THZ1 binding to CDK12 in human Jurkat cells assessed as reduction in cyclin K pull down at 1 uM preincubated for 6 hrs followed by biotinylated-THZ1 addition by Western blotting based pull-down assay	Homo sapiens	6	ALA4739714	cell-based format	Patent Bioactivity Data
20.	ALA4739833	B	Binding affinity to CDK12 in human Jurkat cells assessed as target occupancy level incubated for 2 hrs by measuring Bio-THZ531 bound protein level by spectrophotometry based plate based target engagement assay	Homo sapiens	2	ALA4739718	cell-based format	Patent Bioactivity Data