| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3585104 | A | Binding affinity to human saposin B expressed in Escherichia coli BL21 Gold (DE3) at 120 uM using 15 uM sapB and 50 mM MES and 4% THF at pH 5.5 and 25 degC by ITC method | Homo sapiens | 1 | ALA3580619 | assay format | Scientific Literature | |
| 2. | ALA3585105 | A | Binding affinity to human saposin B expressed in Escherichia coli BL21 Gold (DE3) at 154 uM using 25 to 27 uM sapB and 50 mM MES and 50% DMF at pH 5.5 and 25 degC by ITC method | Homo sapiens | 1 | ALA3580619 | assay format | Scientific Literature | |
| 3. | ALA3585106 | A | Binding affinity to human saposin B expressed in Escherichia coli BL21 Gold (DE3) at 150 uM using 15 to 20 uM sapB and 50 mM MES and 10% DMF at pH 5.5 and 25 degC by ITC method | Homo sapiens | 1 | ALA3580619 | assay format | Scientific Literature | |
| 4. | ALA3585107 | A | Binding affinity to human saposin B expressed in Escherichia coli BL21 Gold (DE3) at 150 um using 20 uM SapB and 20 mM phosphate and 30% DMF at pH 7.4 and 25 degC by ITC method | Homo sapiens | 1 | ALA3580619 | assay format | Scientific Literature | |
| 5. | ALA4700523 | B | Binding affinity to human saposin D (1 to 81 residues) expressed in Escherichia coli assessed as decrease in BODIPY-TR labeled ceramide solubilization in sodium phosphate buffer measured after 30 mins by fluorescence assay | Homo sapiens | 1 | ALA4699434 | assay format | Scientific Literature | |
| 6. | ALA4700524 | B | Binding affinity to human saposin D (1 to 81 residues) expressed in Escherichia coli assessed as association constant at equilibrium at 25 degree C by electronic absorption spectroscopy | Homo sapiens | 1 | ALA4699434 | assay format | Scientific Literature | |
| 7. | ALA4700525 | B | Binding affinity to human saposin D (1 to 81 residues) expressed in Escherichia coli at 25 degree C by electronic absorption spectroscopy | Homo sapiens | 1 | ALA4699434 | assay format | Scientific Literature | |
| 8. | ALA4700526 | B | Binding affinity to human saposin D (1 to 81 residues) expressed in Escherichia coli assessed as association constant at equilibrium at 25 degree C by fluorescence quenching spectroscopy | Homo sapiens | 1 | ALA4699434 | assay format | Scientific Literature | |
| 9. | ALA4700527 | B | Binding affinity to human saposin D (1 to 81 residues) expressed in Escherichia coli at 25 degree C by fluorescence quenching spectroscopy | Homo sapiens | 1 | ALA4699434 | assay format | Scientific Literature | |
| 10. | ALA4700528 | B | Binding affinity to human saposin D (1 to 81 residues) expressed in Escherichia coli complexed with CerOH assessed as decrease in ceramide hydrolysis using ceramide as counter-substrate measured after 24 hrs in presence of acid ceramidase by fluorescence quenching spectroscopy | Homo sapiens | 1 | ALA4699434 | assay format | Scientific Literature | |
| 11. | ALA4700529 | B | Binding affinity to human saposin D (1 to 81 residues) expressed in Escherichia coli complexed with CerPO4 assessed as decrease in ceramide hydrolysis using ceramide as counter-substrate preincubated the protein-ceramide complex for 30 mins measured after 24 hrs in presence of acid ceramidase by HPLC analysis | Homo sapiens | 1 | ALA4699434 | assay format | Scientific Literature | |
| 12. | ALA4700530 | B | Binding affinity to human saposin D (1 to 81 residues) expressed in Escherichia coli assessed as inhibition of ceramide hydrolysis preincubated for 30 mins followed by ceramide addition measured after 72 hrs by HPLC analysis | Homo sapiens | 1 | ALA4699434 | assay format | Scientific Literature |
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