# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3590857 | B | Inhibition of human GOAT octanoylation activity expressed in insect Sf9 cell membranes using GSSFLCAcDan as substrate after 1 hr by reverse phase HPLC with fluorescence detection method | Homo sapiens | 4 | ALA3588827 | cell-based format | Scientific Literature | |
2. | ALA4482488 | B | Inhibition of human GOAT expressed in Sf9 cell membranes assessed as reduction in ghrelin octanoylation pre-incubated for 15 mins before substrate addition and measured after 3 hrs using octanoyl-CoA and fluorescent ghrelin peptide by HPLC analysis | Homo sapiens | 6 | ALA4482308 | cell-based format | Patent Bioactivity Data | |
3. | ALA4482681 | B | Inhibition of GOAT (unknown origin) expressed in intact rat INS1 cells co-expressing pre-proghrelin incubated for 24 hrs | Homo sapiens | 1 | ALA4482314 | cell-based format | Patent Bioactivity Data | |
4. | ALA4673662 | B | Inhibition of human His-tagged GOAT expressed in baculovirus infected Sf9 cell membrane using desacyl-ghrelin-biotin and octanoyl-CoA as substrate incubated for 1 hr by ELISA | Homo sapiens | 6 | ALA4673189 | cell membrane format | Scientific Literature | |
5. | ALA4673664 | B | Inhibition of GOAT (unknown origin) | Homo sapiens | 1 | ALA4673189 | single protein format | Scientific Literature | |
6. | ALA4673665 | B | Inhibition of human GOAT expressed in baculovirus infected sf9 insect cell membrane using GSSFLC-acrylodan and octanoyl-CoA as substrate preincubated for 30 mins followed by substrate addition and measured after 3 hrs by HPLC analysis | Homo sapiens | 2 | ALA4673189 | cell membrane format | Scientific Literature | |
7. | ALA4673666 | B | Inhibition of GOAT (unknown origin) by fluorescence-based assay | Homo sapiens | 1 | ALA4673189 | single protein format | Scientific Literature | |
8. | ALA4673668 | B | Inhibition of human GOAT expressed in baculovirus infected sf9 insect cell microsomes using biotinylated des-acyl ghrelin and octanoyl-CoA as substrate incubated for 15 mins by HTRF assay | Homo sapiens | 2 | ALA4673189 | microsome format | Scientific Literature | |
9. | ALA4673673 | B | Inhibition of human His-tagged GOAT expressed in baculovirus infected Sf9 cell membrane using biotinylated ghrelin peptide/octanoyl-CoA/palmitoyl CoA as substrate incubated for 80 mins by TR-FRET assay | Homo sapiens | 1 | ALA4673189 | cell membrane format | Scientific Literature | |
10. | ALA4673674 | B | Inhibition of human GOAT expressed in HEK293 cells harboring -preproghrelin in presence of Octanoate-BSA incubated for 5 hr by ELISA | Homo sapiens | 5 | ALA4673189 | cell-based format | Scientific Literature | |
11. | ALA5052901 | B | Inhibition of His-tagged human GOAT expressed in baculovirus expression system using biotinylated ghrelin peptide as substrate incubated 80 mins and measured after 40 mins by TR-FRET assay | Homo sapiens | 4 | ALA5052822 | assay format | Patent Bioactivity Data | |
12. | ALA5053172 | B | Inhibition of His-tagged human GOAT expressed in baculovirus expression system using biotinylated ghrelin peptide as substrate incubated 80 mins and measured after 40 mins by TR-FRET assay | Homo sapiens | 17 | ALA5052837 | assay format | Patent Bioactivity Data | |
13. | ALA5053471 | B | Inhibition of human GOAT expressed in baculovirus infected Sf9 cells at 10 uM using octanoyl-CoA and ghrelin-biotin as substrate preincubated for 20 mins followed by substrate addition and measured after = > 1 hr by HTRF assay relative to control | Homo sapiens | 75 | ALA5053468 | cell-based format | Patent Bioactivity Data |