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# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3591245 | Binding | Inhibition of Plasmodium falciparum DHODH expressed in Escherichia coli by 2,5-dichloroindophenol-based spectrophotometric method | Plasmodium falciparum | 2 | ALA3588748 | assay format | Scientific Literature | |
2. | ALA3705388 | Binding | Inhibiton Assay: For studying inhibition of Plasmodium or human DHODH enzyme, two assays that are in routine use are described, for example, in Baldwin, et al. (2002) JBiol Chem., 277, 41827-41834, and Baldwin, et al. (2005) J. Biol. Chem., 280. 21847-21853.Briefly, this colorimetric assay monitors the reduction of 2,6-dichloroindophenol (DCIP) at 600 nm (e = 18.8 mM-1cm-1) for measuring DHOD inhibition. The assay was carried out using a solution containing 100 mM HEPES, pH 8.0, 150 mM NaCl, 10% glycerol, 0.1% Triton X-100, 20 micro molar CoQD (coenzyme QD), 200 micro molar L-dihydroorotate, and 120 micro molar DCIP. Reactions are initiated by addition of enzyme to a final concentration in the range of about 5 nM to about 50 nM while maintaining the temperature of a circulating water bath at 25° C. Alternatively, for potent compounds, activity was determined by directly measuring the production of orotic acid at 296 nm (ε = 4.3 mM-1 cm-1). | Plasmodium falciparum | 53 | ALA3639309 | single protein format | BindingDB Database | |
3. | ALA911117 | Binding | Inhibition of Plasmodium falciparum DHODH H185A mutant | Plasmodium falciparum | 4 | ALA1139229 | assay format | Scientific Literature | |
4. | ALA911119 | Binding | Inhibition of Plasmodium falciparum DHODH F188A mutant | Plasmodium falciparum | 7 | ALA1139229 | assay format | Scientific Literature | |
5. | ALA911120 | Binding | Inhibition of Plasmodium falciparum DHODH R265K mutant | Plasmodium falciparum | 4 | ALA1139229 | assay format | Scientific Literature | |
6. | ALA965255 | Binding | Inhibition of Plasmodium falciparum DHODH F227A mutant | Plasmodium falciparum | 1 | ALA1150779 | assay format | Scientific Literature | |
7. | ALA965256 | Binding | Inhibition of Plasmodium falciparum DHODH R265A mutant | Plasmodium falciparum | 1 | ALA1150779 | assay format | Scientific Literature | |
8. | ALA2187985 | Binding | Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase F188Y mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis | Plasmodium falciparum | 4 | ALA2177061 | assay format | Scientific Literature | |
9. | ALA2187986 | Binding | Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase C276Y mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis | Plasmodium falciparum | 4 | ALA2177061 | assay format | Scientific Literature | |
10. | ALA2187987 | Binding | Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase C276F mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis | Plasmodium falciparum | 4 | ALA2177061 | assay format | Scientific Literature | |
11. | ALA2187988 | Binding | Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase C276A mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis | Plasmodium falciparum | 1 | ALA2177061 | assay format | Scientific Literature | |
12. | ALA2187989 | Binding | Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase H185A mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis | Plasmodium falciparum | 4 | ALA2177061 | assay format | Scientific Literature |