#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA911117	B	Inhibition of Plasmodium falciparum DHODH H185A mutant	Plasmodium falciparum	4		assay format	Scientific Literature
2.	ALA911119	B	Inhibition of Plasmodium falciparum DHODH F188A mutant	Plasmodium falciparum	7		assay format	Scientific Literature
3.	ALA911120	B	Inhibition of Plasmodium falciparum DHODH R265K mutant	Plasmodium falciparum	4		assay format	Scientific Literature
4.	ALA965255	B	Inhibition of Plasmodium falciparum DHODH F227A mutant	Plasmodium falciparum	1		assay format	Scientific Literature
5.	ALA965256	B	Inhibition of Plasmodium falciparum DHODH R265A mutant	Plasmodium falciparum	1		assay format	Scientific Literature
6.	ALA2187985	B	Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase F188Y mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis	Plasmodium falciparum	4		assay format	Scientific Literature
7.	ALA2187986	B	Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase C276Y mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis	Plasmodium falciparum	4		assay format	Scientific Literature
8.	ALA2187987	B	Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase C276F mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis	Plasmodium falciparum	4		assay format	Scientific Literature
9.	ALA2187988	B	Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase C276A mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis	Plasmodium falciparum	1		assay format	Scientific Literature
10.	ALA2187989	B	Inhibition of Plasmodium falciparum dihydrofolate dehydrogenase H185A mutant expressed in Escherichia coli BL21(DE3) using dihydrofolate as substrate at 100 uM by DCIP-based colorimetric analysis	Plasmodium falciparum	4		assay format	Scientific Literature
11.	ALA3591245	B	Inhibition of Plasmodium falciparum DHODH expressed in Escherichia coli by 2,5-dichloroindophenol-based spectrophotometric method	Plasmodium falciparum	2		assay format	Scientific Literature
12.	ALA3705388	B	Inhibiton Assay: For studying inhibition of Plasmodium or human DHODH enzyme, two assays that are in routine use are described, for example, in Baldwin, et al. (2002) JBiol Chem., 277, 41827-41834, and Baldwin, et al. (2005) J. Biol. Chem., 280. 21847-21853.Briefly, this colorimetric assay monitors the reduction of 2,6-dichloroindophenol (DCIP) at 600 nm (e = 18.8 mM-1cm-1) for measuring DHOD inhibition. The assay was carried out using a solution containing 100 mM HEPES, pH 8.0, 150 mM NaCl, 10% glycerol, 0.1% Triton X-100, 20 micro molar CoQD (coenzyme QD), 200 micro molar L-dihydroorotate, and 120 micro molar DCIP. Reactions are initiated by addition of enzyme to a final concentration in the range of about 5 nM to about 50 nM while maintaining the temperature of a circulating water bath at 25° C. Alternatively, for potent compounds, activity was determined by directly measuring the production of orotic acid at 296 nm (ε = 4.3 mM-1 cm-1).	Plasmodium falciparum	53		single protein format	BindingDB Database