#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3578971	Functional	Inhibition of RANKL-induced fusion of preosteoclast derived from ICR mouse bone marrow-derived macrophages assessed as formation of tartrate-resistant acid phosphatase-positive multinucleated osteoclast preincubated for 30 mins with M-CSF followed by RANKL induction measured after 1 day by TRAP staining	Mus musculus	1	ALA3576801	cell-based format	Scientific Literature
2.	ALA3578973	Functional	Inhibition of RANKL-induced migration of preosteoclast derived from ICR mouse bone marrow-derived macrophages after 8 hrs by Boyden chamber assay	Mus musculus	1	ALA3576801	cell-based format	Scientific Literature
3.	ALA3583002	Functional	Antiosteoclastogenic activity in ICR mouse bone marrow-derived preosteoclasts assessed as inhibition of RANKL/M-CSF-induced osteoclast formation at 10 uM after 5 days by TRAP staining	Mus musculus	1	ALA3580547	cell-based format	Scientific Literature
4.	ALA3583006	Binding	Inhibition of RANKL-induced IKK phosphorylation in mouse RAW264.7 cells at 10 uM preincubated for 2 hrs followed by RANKL induction measured over 60 mins by Western blot analysis	Mus musculus	1	ALA3580547	cell-based format	Scientific Literature
5.	ALA3599997	Binding	Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as reduction of TRAP-positive multinucleated cells at 5 uM after 4 days by light microscopic analysis relative to control	Mus musculus	12	ALA3596168	cell-based format	Scientific Literature
6.	ALA3599999	Binding	Inhibition of recombinant mouse RANKL-induced TRAP activity in mouse RAW264.7 cells using 4-nitrophenyl phosphate as TRAP substrate preincubated for 4 days followed by substrate addition measured after 15 mins by ELISA reader analysis	Mus musculus	4	ALA3596168	cell-based format	Scientific Literature
7.	ALA3600000	Binding	Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as suppression of NFATc1 mRNA expression at 2.5 to 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by RT-PCR analysis	Mus musculus	2	ALA3596168	cell-based format	Scientific Literature
8.	ALA3600001	Binding	Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as suppression of c-fos mRNA expression at 2.5 to 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by RT-PCR analysis	Mus musculus	2	ALA3596168	cell-based format	Scientific Literature
9.	ALA3600002	Binding	Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as suppression of TRAP mRNA expression at 2.5 to 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by RT-PCR analysis	Mus musculus	2	ALA3596168	cell-based format	Scientific Literature
10.	ALA3600003	Binding	Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as suppression of cathepsin K mRNA expression at 2.5 to 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by RT-PCR analysis	Mus musculus	2	ALA3596168	cell-based format	Scientific Literature
11.	ALA3600205	Binding	Inhibition of recombinant mouse RANKL-induced NF-kappaB expression in nucleus of mouse RAW264.7 cells at 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by ELISA plate analysis	Mus musculus	1	ALA3596168	cell-based format	Scientific Literature
12.	ALA3600206	Binding	Inhibition of recombinant mouse RANKL-induced NF-kappaB expression in nuclear extracts of mouse RAW264.7 cells assessed as decrease in ratio of NF-kappaB to TBP level at 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by Western blot analysis relative to control	Mus musculus	1	ALA3596168	assay format	Scientific Literature
13.	ALA3600207	Binding	Inhibition of recombinant mouse RANKL-induced NF-kappaB expression in nuclear extracts of mouse RAW264.7 cells assessed as decrease in ratio of NFATc1 to TBP level at 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by Western blot analysis relative to control	Mus musculus	1	ALA3596168	assay format	Scientific Literature
14.	ALA3600208	Binding	Inhibition of recombinant mouse RANKL-induced bone resorptive activity of mature osteoclasts derived from mouse RAW264.7 cells assessed as attenuation of resorbing pit formation on dentine slices after 4 days by toluidine blue staining assay	Mus musculus	1	ALA3596168	tissue-based format	Scientific Literature
15.	ALA3611159	Functional	Inhibition of RANKL-mediated osteoclast differentiation in mouse BMMC assessed as formation of TRAP-positive osteoclasts after 4 days	Mus musculus	1	ALA3608225	assay format	Scientific Literature
16.	ALA3611161	Functional	Inhibition of RANKL-mediated osteoclast differentiation in mouse BMMC assessed as disruption of F-actin ring formation after 3 days using phalloidin and DAPI staining	Mus musculus	1	ALA3608225	assay format	Scientific Literature
17.	ALA3611163	Functional	Inhibition of RANKL-mediated osteoclastogenesis in mouse BMMC assessed as reduction of c-Fos protein expression at 100 uM after 12 to 48 hrs by Western blot analysis	Mus musculus	1	ALA3608225	assay format	Scientific Literature
18.	ALA3611164	Functional	Inhibition of RANKL-mediated osteoclastogenesis in mouse BMMC assessed as reduction of NFATc1 protein expression at 100 uM after 12 to 48 hrs by Western blot analysis	Mus musculus	1	ALA3608225	assay format	Scientific Literature
19.	ALA3611165	Functional	Inhibition of RANKL-mediated osteoclastogenesis in mouse BMMC assessed as reduction of c-Fos mRNA expression at 100 uM after 12 to 48 hrs by RT-PCR analysis	Mus musculus	1	ALA3608225	assay format	Scientific Literature
20.	ALA3611166	Functional	Inhibition of RANKL-mediated osteoclastogenesis in mouse BMMC assessed as reduction of NFATc1 mRNA expression at 100 uM after 12 to 48 hrs by RT-PCR analysis	Mus musculus	1	ALA3608225	assay format	Scientific Literature