# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3618962 | Binding | Inhibition of human cloned BCATm expressed in Escherichia coli BL21 DE3 assessed as L-glutamate production from alpha-ketoglutarate after 10 mins by fluorescent assay | Homo sapiens | 41 | ALA3616381 | assay format | Scientific Literature | |
2. | ALA3618965 | Binding | Inhibition of BCATm in differentiated primary human adipocytes assessed as remaining leucine level by reversed phase HPLC analysis | Homo sapiens | 38 | ALA3616381 | assay format | Scientific Literature | |
3. | ALA3625417 | Binding | Inhibition of human BCATm after 10 mins by fluorescent assay | Homo sapiens | 10 | ALA3621062 | single protein format | Scientific Literature | |
4. | ALA3795165 | Binding | Inhibition of human BCATm incubated for 10 mins by Amplex red- based fluorescence analysis | Homo sapiens | 42 | ALA3792277 | single protein format | Scientific Literature | |
5. | ALA3812805 | Binding | Inhibition of human BCATm (28 to 392 residues) using L-Leucine and alpha-ketogluterate as substrate assessed as L-glutamate production after 10 mins by Amplex red dye based L-GOx and HRP enzyme coupled assay | Homo sapiens | 24 | ALA3808314 | single protein format | Scientific Literature | |
6. | ALA3812806 | Binding | Inhibition of BCATm in differentiated primary human adipocytes using L-Serine and L-Leucine as substrate after overnight incubation by reverse-phase HPLC method | Homo sapiens | 8 | ALA3808314 | assay format | Scientific Literature | |
7. | ALA4885535 | Binding | Biochemical assay (BCAT2) | Homo sapiens | 2 | ALA4507325 | single protein format | Donated Chemical Probes - SGC Frankfurt | |
8. | ALA4885539 | Binding | Cellular mechanistic assay – BCAA measurement (MDA-MB-231 cells) | Homo sapiens | 1 | ALA4507325 | cell-based format | Donated Chemical Probes - SGC Frankfurt | |
9. | ALA5217582 | Binding | Inhibition of human recombinant BCAT2 using leucine and alpha-KG as substrate in presence of NADH by fluorescence assay | Homo sapiens | 27 | ALA5214979 | single protein format | Scientific Literature |