#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3626666	Binding	Competitive inhibition of human FolD dehydrogenase activity	Homo sapiens	1	ALA3621150	single protein format	Scientific Literature
2.	ALA4022808	Binding	Inhibition of MTHFD2 (unknown origin) using NAD/5,10-methylene THF as substrate after 30 mins by HPLC method	Homo sapiens	1	ALA4020815	single protein format	Scientific Literature
3.	ALA4338134	Binding	Inhibition of recombinant MTHFD2 (unknown origin) using THF as substrate incubated for 30 mins in presence of NAD	Homo sapiens	23	ALA4334513	single protein format	Scientific Literature
4.	ALA4434260	Binding	Inhibition of His-tagged human MTHFD2 ( 36 to 350 residues) expressed in Escherichia coli BL21 (DE3) pre-incubated for 10 mins before folitixorin and NAD+ addition measured after 15 mins by NAD(P)H-Glo detection reagent based luminescence assay	Homo sapiens	1	ALA4433244	assay format	Scientific Literature
5.	ALA4434262	Binding	Inhibition of recombinant MTHFD2 (unknown origin) assessed as reduction in methenyl-THF formation using tetrahydrofolate, NAD and formaldehyde incubated for 30 mins by NAD-dependent dehydrogenase assay	Homo sapiens	28	ALA4433244	single protein format	Scientific Literature
6.	ALA5031410	Binding	Inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells using tetrahydrofolate as substrate preincubated for 10 min in presence of NAD+ followed by addition of substrate and further incubated for 10 mins	Homo sapiens	11	ALA5030528	cell-based format	Scientific Literature
7.	ALA5031412	Binding	Inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells catalytic activity using varying levels of tetrahydrofolate as substrate	Homo sapiens	3	ALA5030528	cell-based format	Scientific Literature
8.	ALA5031413	Binding	Competitive inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as increase in Km using varying levels of tetrahydrofolate as substrate by double-reciprocal plot analysis	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature
9.	ALA5031414	Binding	Competitive inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as effect on Vmax using varying levels of tetrahydrofolate as substrate by double-reciprocal plot analysis	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature
10.	ALA5031415	Binding	Uncompetitive inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as decrease in Km by using varying levels of tetrahydrofolate as substrate by double-reciprocal plot analysis	Homo sapiens	2	ALA5030528	cell-based format	Scientific Literature
11.	ALA5031416	Binding	Uncompetitive inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as decrease in Vmax using varying levels of tetrahydrofolate as substrate by double-reciprocal plot analysis	Homo sapiens	2	ALA5030528	cell-based format	Scientific Literature
12.	ALA5031417	Binding	Binding affinity to human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as induction of conformational changes	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature
13.	ALA5031418	Binding	Binding affinity to human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as induction of betae-alphaE loop shift in monomer A	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature
14.	ALA5031419	Binding	Binding affinity to human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as destabilization of betae-alphaE loop shift in monomer A	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature
15.	ALA5031420	Binding	Binding affinity to human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as movement of alphaD2-alphaD3 loop in monomer B	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature