| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3626967 | B | Inhibition of N-terminal His-tagged human recombinant ALKBH5 (66 to 292 residues) catalytic domain expressed in Escherichia coli BL21 assessed as reduction in 5-mer ssRNA (5'-GGm6ACU-3') demethylation incubated for 6 mins by MALDI-TOF mass spectrometry | Homo sapiens | 1 | ALA3621117 | assay format | Scientific Literature | |
| 2. | ALA4617343 | B | Inhibition of human recombinant ALKBH5 demethylase activity assessed as increase in methylated 6A-RNA level incubated for 30 min by dot blot analysis | Homo sapiens | 1 | ALA4616607 | single protein format | Scientific Literature | |
| 3. | ALA4617344 | B | Inhibition of human recombinant ALKBH5 demethylase activity assessed as increase in methylated 6A-RNA level at 10 to 25 uM incubated for 30 min by dot blot analysis relative to control | Homo sapiens | 1 | ALA4616607 | single protein format | Scientific Literature | |
| 4. | ALA4617345 | B | Inhibition of ALKBH5 demethylase activity in human U87MG cells assessed as decrease in CD73 expression measured after 1 to 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4616607 | cell-based format | Scientific Literature | |
| 5. | ALA4617346 | B | Inhibition of ALKBH5 demethylase activity in human U87MG cells assessed as decrease in CD73 expression at 10 uM measured after 2 hrs by Western blot analysis | Homo sapiens | 1 | ALA4616607 | cell-based format | Scientific Literature | |
| 6. | ALA4617347 | B | Inhibition of ALKBH5 demethylase activity in human U87MG cells assessed as decrease in CD73 expression at 10 uM measured after 1 hr by Western blot analysis | Homo sapiens | 1 | ALA4616607 | cell-based format | Scientific Literature | |
| 7. | ALA4715235 | B | Inhibition of ALKBH5 demethylase activity (unknown origin) assessed as increase in methylated 6A-RNA level incubated for 2 hrs by colorimetric analysis | Homo sapiens | 2 | ALA4706755 | single protein format | Scientific Literature | |
| 8. | ALA4836324 | B | Inhibition of ALKBH5 (unknown origin) by Succinate-Glo assay | Homo sapiens | 1 | ALA4834445 | single protein format | Scientific Literature | |
| 9. | ALA4836331 | B | Binding affinity to ALKBH5 (unknown origin) by differential scanning fluorimetry | Homo sapiens | 1 | ALA4834445 | single protein format | Scientific Literature | |
| 10. | ALA5115879 | B | Inhibition of human full length N-terminal His6-tagged ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) Rosetta cells | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 11. | ALA5115882 | B | Inhibition of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells using FAM-labeled ssRNA (5'-CUCGAUACG(m^6A) UCCGGUCAAA-3') as substrate incubated for 60 mins by fluorescence polarization assay | Homo sapiens | 51 | ALA5113412 | cell-based format | Scientific Literature | |
| 12. | ALA5115883 | B | Stabilization of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as melting temperature measured at 25 to 95 degree C by differential scanning fluorimetry assay | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 13. | ALA5115884 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 100 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 14. | ALA5115885 | B | Inhibition of of human recombinant ALKBH5 (66 to 292 residues) mediated demethylation of m6A substrate expressed in Escherichia coli BL21 (DE3) cells incubated for 2 hrs by dot blot assay | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 15. | ALA5115890 | B | Binding affinity to human recombinant ALKBH5 in human HepG2 cells assessed as thermal stability at 100 uM measured for 3 hrs by cellular thermal shift assay | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 16. | ALA5115891 | B | Inhibition of human recombinant ALKBH5 in human HepG2 cells assessed as increase in m6A level incubated for 72 hrs by dot blot assay | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 17. | ALA5115892 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 50 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 18. | ALA5115893 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 25 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 19. | ALA5115894 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 6.25 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 20. | ALA5115895 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 1.56 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature |
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