Inhibition of MALT1 in human Jurkat cell lysate preincubated for 2 hrs followed by addition of 25 uM Ac-LRSR-AMC substrate for 3 hrs by fluorescence based assay
Inhibition of MALT1 C domain (329 to 824 residues) (unknown origin) using Ac-Trp-Leu-Arg-Ser-Arg-Cys(PT14)-NH2 as substrate preincubated for 60 mins followed by substrate addition measured after 60 mins by fluorescence lifetime-based microtitre plate reader analysis
Inhibition of MALT1 (unknown origin) expressed in human Jurkat Clone K22 29Q_H23 cells assessed as IL2 production preincubated for 30 mins followed by PMA stimulation for 5.5 hrs by luciferase reporter gene assay
Binding affinity to recombinant GST-Malt1 (325 to 760 residues) (unknown origin) assessed as protein labeling at 0.1 to 1 uM after 1 hr by SDS-PAGE based activity-based protein profiling assay
Binding affinity to recombinant GST-Malt1 (325 to 760 residues) (unknown origin) assessed as protein labeling at 0.05 to 0.5 uM after 1 hr by SDS-PAGE based activity-based protein profiling assay
Binding affinity to recombinant GST-Malt1 (325 to 760 residues) (unknown origin) assessed as protein labeling at 1 uM after 1 hr by SDS-PAGE based activity-based protein profiling assay
Binding affinity to Malt1 in phorbol 12-myristate 13-acetate/ionomycin stimulated human Jurkat T cells assessed as protein labeling at 1 uM after 50 mins by SDS-PAGE based activity-based protein profiling assay
Binding affinity to Malt1 in phorbol 12-myristate 13-acetate/ionomycin stimulated human Jurkat T cells assessed as protein labeling at 3 uM after 50 mins by SDS-PAGE based activity-based protein profiling assay
Binding affinity to Flag-tagged Malt1 (unknown origin) overexpressed in human Jurkat T cells assessed as protein labeling at 1 uM after 50 mins by Western blot analysis
Inhibition of recombinant Malt1 (unknown origin) at 10 to 1000 uM preincubated for 1 hr followed by (S)-1-((6-(4-(4-(5,5-Difluoro-3,7-bis(4-methoxyphenyl)-5H-4l4,5l4-dipyrrolo[1,2-c:20,10-f][1,3,2]diazaborinin-10-yl)butyl)-1H-1,2,3-triazol-1-yl)hexanoyl)-L-valyl-L-arginyl)-N-((S)-1-fluoro-6-guanidino-2-oxohexan-3-yl)pyrrolidine-2-carboxamide addition and incubated for 1 hr by SDS-PAGE based competitive activity-based protein profiling assay
Inhibition of recombinant Malt1 (unknown origin) at 1 to 100 uM preincubated for 1 hr followed by (S)-1-((6-(4-(4-(5,5-Difluoro-3,7-bis(4-methoxyphenyl)-5H-4l4,5l4-dipyrrolo[1,2-c:20,10-f][1,3,2]diazaborinin-10-yl)butyl)-1H-1,2,3-triazol-1-yl)hexanoyl)-L-valyl-L-arginyl)-N-((S)-1-fluoro-6-guanidino-2-oxohexan-3-yl)pyrrolidine-2-carboxamide addition and incubated for 1 hr by SDS-PAGE based competitive activity-based protein profiling assay
Binding affinity to Malt1 in phorbol 12-myristate 13-acetate/ionomycin stimulated human Jurkat T cells assessed as protein labeling at 1 to 3 uM after 50 mins by SDS-PAGE based activity-based protein profiling assay
Inhibition of MALT1 in PMA/ionomycin-stimulated human Jurkat T cells assessed as reduction in RelB cleavage at 2 uM pretreated for 4 hrs followed by 1 hr incubation with protease inhibitor MG132 and subsequent PMA/ionomycin-stimulation measured after 1 hr by Western blot analysis
Inhibition of MALT1 (unknown origin) using Ac-Trp-Leu-Arg-Ser-Arg'Cys(PT14)-NH2 as substrate preincubated for 60 mins followed by substrate addition measured after 60 mins by fluorescence assay
Inhibition of cIAP2 fused MALT1 (unknown origin) expressed in HEK293 cells assessed as suppression of NF-kB activation after 24 hrs by luciferase reporter gene assay