# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3636406 | B | Inhibition of microtubule-stimulated KIF15 motor domain (1 to 372) (unknown origin) ATPase activity at 6.3 uM | Homo sapiens | 2 | ALA3632570 | single protein format | Scientific Literature | |
2. | ALA3860180 | B | Inhibition of basal ATPase activity of human Kif15 at 200 uM by pyruvate kinase/lactate dehydrogenase-linked assay | Homo sapiens | 1 | ALA3860026 | single protein format | Scientific Literature | |
3. | ALA3860195 | B | Inhibition of microtubule-stimulated ATPase activity of human Kif15 at 200 uM by pyruvate kinase/lactate dehydrogenase-linked assay | Homo sapiens | 1 | ALA3860026 | single protein format | Scientific Literature | |
4. | ALA4118998 | B | Binding affinity to KIF15 in human NCI-H358 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | ALA4118021 | cell-based format | Scientific Literature | |
5. | ALA4372725 | B | Inhibition of recombinant His6-tagged Kif15 ATPase activity (N420 residues) (unknown origin) expressed in HeLa cells at 10 uM using microtubule as substrate preincubated for 15 mins followed by ATP addition and measured after 20 mins by ADP-Glo luminescence assay relative to control | Homo sapiens | 2 | ALA4371054 | cell-based format | Scientific Literature | |
6. | ALA4372726 | B | Inhibition of recombinant His6-tagged Kif15 ATPase activity (N420 residues) (unknown origin) expressed in HeLa cells using microtubule as substrate preincubated for 15 mins followed by ATP addition and measured after 20 mins by ADP-Glo luminescence assay | Homo sapiens | 2 | ALA4371054 | cell-based format | Scientific Literature | |
7. | ALA4372727 | B | Inhibition of recombinant His6-tagged Kif15 (N700 residues) (unknown origin) expressed in HeLa cells assessed as reduction in Alexa-594 labelled microtubule gliding measured every 2 secs for 20 secs by epifluorescence microscopic analysis | Homo sapiens | 2 | ALA4371054 | cell-based format | Scientific Literature | |
8. | ALA4372728 | B | Reversible inhibition of recombinant His6-tagged Kif15 (N700 residues) (unknown origin) expressed in HeLa cells assessed as reduction in Alexa-594 labelled microtubule gliding at 10 uM treated for 1 min followed by compound washout and measured every 2 secs for 1 min by epifluorescence microscopic analysis | Homo sapiens | 2 | ALA4371054 | cell-based format | Scientific Literature | |
9. | ALA4372729 | B | Inhibition of recombinant His6-tagged Kif15 (N700 residues) (unknown origin) expressed in HeLa cells assessed as reduction in Alexa-594 labelled microtubule gliding at 30 uM measured every 2 secs for 20 secs by epifluorescence microscopic analysis relative to control | Homo sapiens | 1 | ALA4371054 | cell-based format | Scientific Literature | |
10. | ALA4372733 | B | Inhibition of Kif15 in TP53-deficient human RPE1 cells assessed as cells with bipolar pre-anaphase microtubule structure at 25 uM after 24 hrs by Hoechst 33342 staining based immunofluorescence microscopic analysis (Rvb = 90 to 94%) | Homo sapiens | 2 | ALA4371054 | assay format | Scientific Literature | |
11. | ALA4372734 | B | Inhibition of Kif15 in TP53-deficient human RPE1 cells assessed as reduction in spindle-bound Kif15 level at 25 uM after 24 hrs by Hoechst 33342 staining based immunofluorescence microscopic analysis relative to control | Homo sapiens | 2 | ALA4371054 | assay format | Scientific Literature | |
12. | ALA4372735 | B | Inhibition of Kif15 in TP53-deficient human RPE1 cells assessed as reduction in Kif15 level at 25 uM after 24 hrs by Western blot analysis relative to control | Homo sapiens | 2 | ALA4371054 | assay format | Scientific Literature | |
13. | ALA4372736 | B | Inhibition of Kif15 in TP53-deficient human RPE1 cells assessed as effect on spindle length at 25 uM after 24 hrs by Hoechst 33342 staining based immunofluorescence microscopic analysis (Rvb = 11.4 +/- 0.2 um) | Homo sapiens | 2 | ALA4371054 | assay format | Scientific Literature | |
14. | ALA4372737 | B | Inhibition of Kif15 in K51-resistant human KIRC1 cells assessed as cells with monopolar spindles at 25 uM after 24 hrs by Hoechst 33342 staining based immunofluorescence microscopic analysis (Rvb = 43 to 49%) | Homo sapiens | 2 | ALA4371054 | assay format | Scientific Literature | |
15. | ALA4372738 | B | Inhibition of Kif15 in K51-resistant human KIRC1 cells assessed as increase in cells with monopolar spindles after 24 hrs by Hoechst 33342 staining based immunofluorescence microscopic analysis | Homo sapiens | 1 | ALA4371054 | assay format | Scientific Literature | |
16. | ALA4372739 | B | Inhibition of recombinant His6-tagged Kif15 (N700 residues) (unknown origin) expressed in HeLa cells assessed as reduction in Alexa-594 labelled microtubule gliding at 750 nM measured every 2 secs for 20 secs by epifluorescence microscopic analysis (Rvb = 1%) | Homo sapiens | 26 | ALA4371054 | cell-based format | Scientific Literature | |
17. | ALA4372740 | B | Inhibition of recombinant C-terminal truncated His6-tagged Kif15 (N700 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in microtubule gliding measured up to 2 mins | Homo sapiens | 1 | ALA4371054 | assay format | Scientific Literature | |
18. | ALA4372741 | B | ATP non-competitive inhibition of recombinant His6-tagged Kif15 ATPase activity (N700 residues) (unknown origin) expressed in HeLa cells at 0.5 to 2 uM using microtubule as substrate preincubated for 30 mins followed by varying levels of ATP addition by double reciprocal Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4371054 | cell-based format | Scientific Literature | |
19. | ALA4372742 | B | ATP non-competitive inhibition of recombinant His6-tagged Kif15 ATPase activity (N700 residues) (unknown origin) expressed in HeLa cells at 0.5 to 2 uM using varying levels of microtubule as substrate preincubated for 30 mins followed by ATP addition by double reciprocal Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4371054 | cell-based format | Scientific Literature | |
20. | ALA4372743 | B | ATP competitive inhibition of recombinant His6-tagged Kif15 ATPase activity (N700 residues) (unknown origin) expressed in HeLa cells at 5 uM using microtubule as substrate preincubated for 30 mins followed by varying levels of ATP addition by double reciprocal Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4371054 | cell-based format | Scientific Literature |