#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705172	Binding	Radio Ligand Binding Assay: Serial dilutions of compounds (stock in 10 mM DMSO) are prepared by first diluting the compounds in DMSO followed by a 1:50 dilution into assay buffer (10 mM HEPES, pH 8.0, 100 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.5% fatty acid-free BSA, 0.05% Tween-20). The radioligand [3H]4 (example 39) (specific activity 1500 GBq/mmol) is diluted directly into the assay buffer immediately before use to obtain a 20 nM solution. The desired amount of membranes (20 μg/well) is diluted with assay buffer. 50 μL of pre-diluted compound and 50 μL of [3H]4 (example 39) is placed into the bottom of a 96-well well plate. 100 μL of the membrane-suspension is added and the plate stirred for 60 minutes. The reaction is stopped by transfer onto the filter of a 96-well GF/C filter plate (soaked for 1 hour in 0.25% PEI) using a cell harvester.	Homo sapiens	37	ALA3638598	assay format	BindingDB Database
2.	ALA3734137	Functional	Antagonist activity at human GPR4 expressed in KJMGER8 cells assessed as inhibition of 17beta-estradiol-induced response after 6 hrs by steady glo luciferase reporter assay	Homo sapiens	9	ALA3727352	cell-based format	Patent Bioactivity Data
3.	ALA3811134	Functional	Antagonist activity at N-terminal HA-tagged GPR4 (unknown origin) expressed in HEK293 cells assessed as pH dependent cAMP response element-driven transcriptional activity ratio at 10 uM at pH 7.2 incubated for 6 hrs by dual luciferase reporter gene assay relative to untreated control	Homo sapiens	18	ALA3808277	cell-based format	Scientific Literature
4.	ALA3813382	Functional	Antagonist activity at N-terminal HA-tagged GPR4 (unknown origin) expressed in HEK293 cells assessed as inhibition of pH dependent cAMP response element-driven transcriptional activity at pH 7.2 incubated for 6 hrs by dual luciferase reporter gene assay	Homo sapiens	7	ALA3808277	cell-based format	Scientific Literature
5.	ALA4044420	Functional	Antagonist activity at human GPR4 expressed in human HeLa cells assessed as inhibition of pH-induced cAMP accumulation preincubated for 15 mins followed by cAMP addition measured after 60 mins by HTRF method	Homo sapiens	22	ALA4043105	cell-based format	Scientific Literature
6.	ALA4044450	Functional	Antagonist activity at human GPR4 expressed in African green monkey COS7 cells assessed as inhibition of pH 6.5-induced cAMP accumulation at 5 uM after 30 mins by HTRF method relative to control	Homo sapiens	2	ALA4043105	cell-based format	Scientific Literature
7.	ALA4044451	Functional	Antagonist activity at human GPR4 expressed in African green monkey COS7 cells assessed as inhibition of pH 6.5-induced cAMP accumulation after 30 mins by HTRF method	Homo sapiens	2	ALA4043105	cell-based format	Scientific Literature
8.	ALA4178741	Functional	Antagonist activity at human GPR4 expressed in human HeLa cells assessed as inhibition of IBMX-induced cAMP accumulation after 15 mins by HTRF assay	Homo sapiens	13	ALA4177561	cell-based format	Scientific Literature
9.	ALA4178757	Functional	Antagonist activity at human GPR4 expressed in human HeLa cells assessed as inhibition of IBMX-induced cAMP accumulation after 15 mins in presence of 40% rat plasma by HTRF assay	Homo sapiens	3	ALA4177561	cell-based format	Scientific Literature
10.	ALA4178758	Binding	Ratio of IC50 for human GPR4 expressed in human HeLa cells in presence of 40% rat plasma to IC50 for human GPR4 expressed in human HeLa in absence of 40% rat plasma	Homo sapiens	3	ALA4177561	cell-based format	Scientific Literature
11.	ALA4407313	Functional	Positive allosteric modulation of human GPR4 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production at higher concentration in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	4	ALA4406806	cell-based format	Scientific Literature
12.	ALA4407338	Functional	Positive allosteric modulation of human GPR4 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	3	ALA4406806	cell-based format	Scientific Literature
13.	ALA4883452	Binding	PRESTO-Tango GPCRome screening (GPR4)		1	ALA4507309	single protein format	Donated Chemical Probes - SGC Frankfurt