#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3887418	B	Radioligand Binding Assay: The detailed experimental protocols for the radioligand and functional receptor assays are available on the NIMH PDSP website at http://pdsp.med.unc.edu/UNC-CH %20Protocol %20Book.pdf. A. Serotonin receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT3, 5-HT5A, 5-HT6 and 5-HT7. Assay Buffer: Standard Binding Buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM EDTA, pH 7.4) Membrane Fraction Source: Transiently or stably transfected cell lines (e.g., HEK293, COS, CHO, NIH3T3). Protocol adapted from Roth et al. (1986), J. Pharmacol. Exp. Ther., 238(2): 480-485; and Roth et al. (1994), J. Pharmacol. Exp. Ther., 268(3): 1403-1410. Adrenergic Receptors: alpha1A, alpha1B, alpha2A, alpha2B, alpha2C, beta1, beta2, beta3 Assay Buffers: For alpha1 receptors, alpha1 Binding Buffer (20 mM Tris-HCl, 145 mM NaCl, pH 7.4); for alpha2 receptors, alpha2 Binding Buffer (50 mM Tris-HCl, 5 mM MgCl2, pH 7.7); for beta receptors.		2	ALA3886311	assay format	BindingDB Database
2.	ALA5104740	B	Positive allosteric modulation of mouse D5 receptor expressed in HEK293 cells assessed as increase in cyclic AMP production measured after 60 mins in presence of dopamine by HTRF assay	Mus musculus	1	ALA5104100	cell-based format	Scientific Literature
3.	ALA5104741	B	Positive allosteric modulation of mouse D5 receptor expressed in HEK293 cells assessed as increase in cyclic AMP production by measuring maximum efficacy measured after 60 mins in presence of dopamine by HTRF assay relative to control	Mus musculus	1	ALA5104100	cell-based format	Scientific Literature
4.	ALA5104742	F	Agonist activity at mouse D5 receptor expressed in HEK293 cells assessed as increase in cyclic AMP production measured after 60 mins by HTRF assay	Mus musculus	1	ALA5104100	cell-based format	Scientific Literature
5.	ALA5104743	F	Agonist activity at mouse D5 receptor expressed in HEK293 cells assessed as increase in cyclic AMP production by measuring maximum efficacy measured after 60 mins by HTRF assay relative to control	Mus musculus	1	ALA5104100	cell-based format	Scientific Literature