#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3428249	Binding	Inhibition of MAPKAPK2 (unknown origin) assessed as remaining activity at 100 uM by radioactive filter binding assay in presence of [33P]-ATP relative to control	Homo sapiens	16	ALA3425453	single protein format	Scientific Literature
2.	ALA3607974	Binding	Inhibition of human MAPKAPK2	Homo sapiens	1	ALA3603755	single protein format	Scientific Literature
3.	ALA3630367	Binding	Inhibition of MK2 (unknown origin) at 40 uM by scintillation proximity assay	Homo sapiens	17	ALA3627620	single protein format	Scientific Literature
4.	ALA3630368	Binding	Inhibition of MK2 (unknown origin) by scintillation proximity assay	Homo sapiens	30	ALA3627620	single protein format	Scientific Literature
5.	ALA3630369	Binding	Inhibition of MK2 (unknown origin) at 20 uM by scintillation proximity assay	Homo sapiens	16	ALA3627620	single protein format	Scientific Literature
6.	ALA3630370	Binding	Inhibition of MK2 (unknown origin) at 50 uM by scintillation proximity assay	Homo sapiens	2	ALA3627620	single protein format	Scientific Literature
7.	ALA3631011	Binding	Inhibition of MAPKAPK2 (unknown origin) at 1 uM by FRET method	Homo sapiens	1	ALA3627690	single protein format	Scientific Literature
8.	ALA3630841	Binding	Inhibition of MAPKAPK2 (unknown origin) assessed as remaining activity at 10 uM by high-throughput assay relative to control	Homo sapiens	1	ALA3627674	single protein format	Scientific Literature
9.	ALA3631281	Binding	Inhibition of MAPKAPK2 (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
10.	ALA3636795	Binding	Inhibition of MAPKAPK2 (unknown origin) after 120 mins by HotSpot assay	Homo sapiens	1	ALA3632549	single protein format	Scientific Literature
11.	ALA3706225	Binding	IMAP Assay: The ability of compounds to inhibit activated phospho-p38alpha is evaluated using a p38alpha /MK2 and a p38alpha /PRAK cascade assay format. The kinase activity of p38alpha is determined by its ability to phosphorylate GST-MK2 or GST-PRAK. Activation of MK2 or PRAK by p38alpha is quantitated by measuring the phosphorylation of a fluorescently-labeled, MK2/PRAK specific peptide substrate, Hsp27 peptide (FITC-KKKALSRQLSVAA). The phosphorylation of the Hsp27 peptide is quantified using IMAP technology (Molecular Devices, Sunnyvale Calif.). Kinase reactions are carried out in a 384-well plate (Greiner, 781280) in 20 mM HEPES pH 7.5, 10 mM MgCl2, 0.01% Triton X-100, 0.01% BSA, 1 mM DTT, and 2% DMSO. The inhibitor concentration is varied between 0.02-30,000 nM, while the Hsp27 peptide substrate and MgATP are held constant at 1 uM and 10 uM, respectively. Activated p38alpha is added to a final concentration of 30 uM for reactions with nonphosphorylated 1 nM GST-MK2.	Homo sapiens	1	ALA3638763	single protein format	BindingDB Database
12.	ALA3734484	Binding	Inhibition of recombinant human MAPKAPK2 using fluorescein-labeled peptide as substrate by fluorescence-electrophoretic mobility shift assay	Homo sapiens	1	ALA3727354	single protein format	Patent Bioactivity Data
13.	ALA3737490	Binding	Inhibition of MAPKAPK2 (unknown origin) at 1 uM by selectscreen kinase profiling assay in presence of ATP	Homo sapiens	1	ALA3734692	single protein format	Scientific Literature
14.	ALA3739150	Binding	Inhibition of MAPKAPK2 (unknown origin) at 200 nM by electrophoretic mobility shift assay	Homo sapiens	2	ALA3734671	single protein format	Scientific Literature
15.	ALA3743115	Binding	Inhibition of MK2 (unknown origin) at 30 uM by microfluidic assay relative to control	Homo sapiens	19	ALA3739348	single protein format	Scientific Literature
16.	ALA3742707	Binding	Inhibition of MAPK-APK2 (unknown origin)	Homo sapiens	1	ALA3739276	single protein format	Scientific Literature
17.	ALA3751219	Binding	Inhibition of MAPKAPK2 (unknown origin) at 1 uM	Homo sapiens	1	ALA3745631	single protein format	Scientific Literature
18.	ALA3750413	Binding	Inhibition of MK2 (unknown origin)	Homo sapiens	1	ALA3745712	single protein format	Scientific Literature
19.	ALA3747982	Binding	Inhibition of human MAPKAPK2 using [KKLNRTLSVA] as substrate	Homo sapiens	1	ALA3745707	single protein format	Scientific Literature
20.	ALA3761590	Binding	Inhibition of MAPKAPK2 (unknown origin) at 500 nM	Homo sapiens	11	ALA3758163	single protein format	Scientific Literature