#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3619996	Binding	Inhibition of recombinant human activated-MMP3 using Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 as fluorogenic substrate incubated for 4 hrs followed by substrate addition measured every 10 secs for 20 mins by fluorometric assay	Homo sapiens	7	ALA3616369	single protein format	Scientific Literature
2.	ALA3707903	Binding	Enzymatic Assay: The products are solubilized in DMSO at a concentration of 10 mM. A serial 3-fold dilution over 10 points is carried out so as to have a concentration range of from 10 uM to 0.5 nM final concentration. The TACE enzyme is an internal production (carried out according to the publication protein Eng Des Sel 2006, 19,155-161) and is added so as to have a signal equivalent to 6 times the background noise in 2 h at 37C. The reaction is carried out in 50 mM Tris buffered medium containing 4% glycerol, pH 7.4. The fluorescent substrate is MCA-Pro-Leu-Ala-Val-(Dpa)-Arg-Ser-Ser-Arg-NH2 (R&D systems, reference: ES003). The substrate is cleaved by the enzyme between the alanine and the valine, thus releasing a fluorescent peptide (excitation: 320 nm, emission: 420 nm). The substrate is used at 40 uM. The reaction is carried out in a final volume of 10 ul (4 ul inhibitor, 4 ul substrate, 2 ul enzyme) in a low volume 384-well plate (Corning reference: 3676).		2	ALA3639319	single protein format	BindingDB Database
3.	ALA3707702	Binding	Inhibition Assay: The inhibition tests and the evaluation of the inhibition constants (Ki) on the various MMPs were carried out as described by Devel et al. (Devel et al. 2006 J. Biol. Chem. (7)).		103	ALA3638630	single protein format	BindingDB Database
4.	ALA3707536	Binding	Enzyme Assay: The molecules are tested in dose-response studies on the following enzymes MMP1, MMP3, MMP9, ADAMS and ADAM10 according to the same protocol as that described for the TACE enzyme in example 28 but with different substrates (MMP R&D system reference: P126-990 and ADAM R&D system reference: ES003).		4	ALA3639220	single protein format	BindingDB Database
5.	ALA3743100	Binding	Inhibition of MMP3 (unknown origin) using Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 as substrate after 3 mins by fluorimetric assay	Homo sapiens	8	ALA3739381	single protein format	Scientific Literature
6.	ALA3755722	Binding	Inhibition of human recombinant MMP3 expressed in Escherichia coli using (5-FAM/QXLTM) FRET peptide as substrate	Homo sapiens	1	ALA3751715	assay format	Scientific Literature
7.	ALA3762360	Binding	Inhibition of MMP-3 (unknown origin)	Homo sapiens	3	ALA3758076	single protein format	Scientific Literature
8.	ALA3761850	Binding	Inhibition of MMP3 (unknown origin) catalytic domain using Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 as substrate preincubated for 60 mins followed by substrate addition by fluorescence assay	Homo sapiens	22	ALA3758105	single protein format	Scientific Literature
9.	ALA702341	Binding	50% inhibition of human recombinant stromelysin (MMP-3, HFS)		19	ALA1130307	single protein format	Scientific Literature
10.	ALA702342	Binding	Inhibition of human recombinant stromelysin (MMP-3, HFS)	Homo sapiens	8	ALA1130307	single protein format	Scientific Literature
11.	ALA702343	Binding	Inhibition of human recombinant stromelysin (MMP-3, HFS) at 100000 nM	Homo sapiens	5	ALA1130307	single protein format	Scientific Literature
12.	ALA710566	Binding	Activity against human stromelysin (MMP-3).		36	ALA1127698	single protein format	Scientific Literature
13.	ALA710567	Binding	Inhibition of fibroblast stromelysin (MMP-3)	Homo sapiens	14	ALA1130917	single protein format	Scientific Literature
14.	ALA710568	Binding	Inhibition of stromelysin-1 (MMP-3).		4	ALA1128968	single protein format	Scientific Literature
15.	ALA710569	Binding	Inhibitory activity against Matrix metalloprotease-3		16	ALA1128973	single protein format	Scientific Literature
16.	ALA710570	Binding	Inhibition of matrix metalloprotease-3 (MMP-3)	Homo sapiens	14	ALA1130916	single protein format	Scientific Literature
17.	ALA710571	Binding	Inhibition of recombinant matrix metalloprotease-3 (MMP-3)	Homo sapiens	2	ALA1132009	single protein format	Scientific Literature
18.	ALA710572	Binding	Inhibition of matrix metalloprotease-3	Homo sapiens	9	ALA1132530	single protein format	Scientific Literature
19.	ALA710573	Binding	Inhibition of matrix metalloprotease-3 (MMP-3)	Homo sapiens	6	ALA1152052	single protein format	Scientific Literature
20.	ALA711196	Binding	Inhibition of Strom-1 MMP-3		21	ALA1132007	single protein format	Scientific Literature