PUBCHEM_BIOASSAY: Late stage assay provider results from the probe development effort to identify inhibitors of GSTO1: Gel-based activity-based protein profiling (ABPP) IC50 Set 2. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID1515, AID1974, AID2175, AID2176, AID449772, AID449773, AID463081, AID463093, AID463094, AID463098, AID463101, AID463102, AID463110]
Binding affinity to GSTO1 cysteine residue in human 786-O cell soluble proteomic lysate at 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis
Binding affinity to GSTO1 C32 residue in human 786-O cell lysate at 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis
Binding affinity to GSTO1 in human A549 cells lysates grown on SILAC media at 10 uM incubated for 1 hr by LC-MS/MS analysis relative to untreated control
Inhibition of CMFDA binding to human N-terminal 6x-His-tagged GSTO1-1 preincubated for 30 mins followed by CMFDA addition and measured after 30 mins by in-gel fluorescence binding assay
Inhibition of CMFDA binding to human N-terminal 6x-His-tagged GSTO1-1 at 10 uM preincubated for 30 mins followed by CMFDA addition and measured after 30 mins in presence of 1 to 10 uM MgCl2 by in-gel fluorescence binding assay
Inhibition of CMFDA binding to human N-terminal 6x-His-tagged GSTO1-1 at 10 uM preincubated for 30 mins followed by CMFDA addition and measured after 30 mins in presence of 1 to 10 uM CuSO4 by in-gel fluorescence binding assay