| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA760819 | B | Inhibition of phosphodiesterase 1C at 100 uM | Bos taurus | 1 | ALA1127705 | single protein format | Scientific Literature | |
| 2. | ALA760820 | B | Inhibition of Phosphodiesterase (PDE Ic) at 100 uM. | Bos taurus | 23 | ALA1127705 | single protein format | Scientific Literature | |
| 3. | ALA760821 | B | Inhibition of phosphodiesterase Ic isoenzyme | Bos taurus | 15 | ALA1127705 | single protein format | Scientific Literature | |
| 4. | ALA966926 | B | Inhibition of PDE1c | 12 | ALA1138608 | single protein format | Scientific Literature | ||
| 5. | ALA1036314 | B | Inhibition of PDE1C | 1 | ALA1151934 | single protein format | Scientific Literature | ||
| 6. | ALA1034522 | B | Inhibition of PDE1C at 10 uM | 3 | ALA1151934 | single protein format | Scientific Literature | ||
| 7. | ALA1045951 | B | Inhibition of PDE1C | 1 | ALA1154619 | single protein format | Scientific Literature | ||
| 8. | ALA1047620 | B | Binding affinity to PDE1C | 1 | ALA1154708 | single protein format | Scientific Literature | ||
| 9. | ALA1047622 | B | Inhibition of human recombinant PDE1C expressed in Sf9 cells by SPA | Homo sapiens | 15 | ALA1154708 | cell-based format | Scientific Literature | |
| 10. | ALA1045409 | B | Inhibition of PDE1C | 2 | ALA1155301 | single protein format | Scientific Literature | ||
| 11. | ALA1074182 | B | Inhibition of PDE1C | 1 | ALA1157659 | single protein format | Scientific Literature | ||
| 12. | ALA1919548 | B | Inhibition of PDE1C | 1 | ALA1914420 | single protein format | Scientific Literature | ||
| 13. | ALA2185161 | B | Inhibition of PDE1C | 6 | ALA2176956 | single protein format | Scientific Literature | ||
| 14. | ALA2185812 | B | Inhibition of human recombinant PDE1C expressed in Sf9 cells using [3H]cAMP as substrate after 30 mins by scintillation proximity assay | Homo sapiens | 15 | ALA2176893 | cell-based format | Scientific Literature | |
| 15. | ALA2433336 | B | Inhibition of PDE1C (unknown origin) | Homo sapiens | 6 | ALA2429765 | single protein format | Scientific Literature | |
| 16. | ALA3369962 | B | Inhibition of PDE1C (unknown origin) at 10 uM | Homo sapiens | 11 | ALA3352060 | single protein format | Scientific Literature | |
| 17. | ALA3602932 | B | Inhibition of human PDE1C1 | Homo sapiens | 1 | ALA3600381 | single protein format | Scientific Literature | |
| 18. | ALA3705328 | B | In Vitro Inhibition Assay: The test substances are dissolved in 100% DMSO and serially diluted to determine their in vitro effect on PDE 9A. 2 uL portions of the diluted substance solutions are introduced into the wells of microtiter plates (Isoplate; Wallac Inc., Atlanta, Ga.). Then 50 uL of a dilution of the PDE9A preparation described above are added. The dilution of the PDE9A preparation is chosen so that less than 70% of the substrate is converted during the subsequent incubation (typical dilution: 1:10000; dilution buffer: 50 mM Tris/HCl pH 7.5, 8.3 mM MgCl2, 1.7 mM EDTA, 0.2% BSA). The substrate, [8-3H] guanosine 3',5'-cyclic phosphate (1 uCi/uL; Amersham Pharmacia Biotech., Piscataway, N.J.) is diluted 1:2000 with assay buffer (50 mM Tris/HCl pH 7.5, 8.3 mM MgCl2, 1.7 mM EDTA) to a concentration of 0.0005 uCi/uL. | 1 | ALA3639087 | single protein format | BindingDB Database | ||
| 19. | ALA3706363 | B | Inhibition Assay: The test substances are dissolved in 100% DMSO and serially diluted to determine their in vitro effect on PDE 9A. Typically, serial dilutions from 200 uM to 1.6 uM are prepared (resulting final concentrations in the assay: 4 uM to 0.032 uM). 2 uL portions of the diluted substance solutions are introduced into the wells of microtiter plates (Isoplate; Wallac Inc., Atlanta, Ga.). Then 50 uL of a dilution of the PDE9A preparation described above are added. The dilution of the PDE9A preparation is chosen so that less than 70% of the substrate is converted during the subsequent incubation (typical dilution: 1:10000; dilution buffer: 50 mM Tris/HCl pH 7.5, 8.3 mM MgCl2, 1.7 mM EDTA, 0.2% BSA). The substrate, [8-3H] guanosine 3',5'-cyclic phosphate (1 uCi/uL; Amersham Pharmacia Biotech., Piscataway, N.J.) is diluted 1:2000 with assay buffer (50 mM Tris/HCl pH 7.5, 8.3 mM MgCl2, 1.7 mM EDTA) to a concentration of 0.0005 uCi/uL. | 1 | ALA3638938 | single protein format | BindingDB Database | ||
| 20. | ALA4122467 | B | Inhibition of full length recombinant human PDE1C using 3',5'-[3H]cAMP as substrate after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4118152 | single protein format | Scientific Literature |
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