# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA697286 | B | Inhibitory activity against histone deacetylases (HDAC1) prepared from mouse melanoma B16/BL6 cells | Mus musculus | 5 | cell-based format | Scientific Literature | ||
2. | ALA697287 | B | Inhibitory concentration gainst Histone deacetylase 1 derived from NIH3T3 cells | Mus musculus | 1 | cell-based format | Scientific Literature | ||
3. | ALA697288 | B | In vitro for anti-HDAC1 (Histone deacetylase 1) activity in mouse A20 cells | Mus musculus | 5 | cell-based format | Scientific Literature | ||
4. | ALA857175 | B | Inhibitory activity against mouse Histone deacetylase 1 (HDAC1) | Mus musculus | 1 | single protein format | Scientific Literature | ||
5. | ALA697289 | B | Binding affinity towards HDAC1 (Histone deacetylase 1) in mouse A20 cells, expressed as binding constant (pKi) | Mus musculus | 5 | cell-based format | Scientific Literature | ||
6. | ALA697292 | B | Inhibition of mouse Histone deacetylase 1 (HDAC1) receptor | Mus musculus | 5 | single protein format | Scientific Literature | ||
7. | ALA697293 | B | Inhibition of mouse HDAC1 | Mus musculus | 5 | single protein format | Scientific Literature | ||
8. | ALA908971 | B | Inhibition of mouse HDAC1 | Mus musculus | 10 | single protein format | Scientific Literature | ||
9. | ALA908242 | B | Inhibition of mouse HDAC1 (mean) | Mus musculus | 3 | single protein format | Scientific Literature | ||
10. | ALA949109 | B | Inhibition of mouse liver HDAC1 | Mus musculus | 6 | assay format | Scientific Literature | ||
11. | ALA3705252 | B | HDAC Inhbition Assay: Assay was done in 96-well black microplate and total volume of the assay was 100 pt. Mouse liver enzyme (10 mg/ml) was diluted 1:6 with HDAC buffer. Enzyme cocktail was made of 10 μL of diluted enzyme and 30 μL of HDAC buffer. 40 μl of enzyme cocktail followed by 10 μL of test compound (1 μM and 10 μM) or buffer (control) was added to each well. The plate was pre-incubated at 37° C. for 5 minutes. The HDAC reaction was started by adding 50 μl of HDAC substrate Boc-Lys (Ac)-AMC (Bachem AG, Switzerland). The plate was incubated at 37° C. for 30 min. The reaction was stopped by adding 100 μL, of Trypsin stop solution and incubating at 37° C. for 15-30 min. Measuring the fluorescence at excitation wavelength of 360 nm and emission wavelength of 460 nm monitored the release of AMC. Buffer alone and substrate alone served as blank. | Mus musculus | 30 | single protein format | BindingDB Database | ||
12. | ALA4353992 | B | Inhibition of HDAC1 in LPS/IFNgamma-stimulated mouse RAW blue cells assessed as upregulation of NFkappaB transcriptional activity by measuring increase in SEAP activity at 1 to 2.5 uM pretreated for 16 hrs followed by LPS stimulation and measured after 4 hrs by quanti blue reagent-based reporter gene assay | Mus musculus | 1 | assay format | Scientific Literature | ||
13. | ALA4353997 | B | Inhibition of HDAC1 in LPS/IFNgamma-stimulated mouse RAW blue cells assessed as upregulation of NFkappaB transcriptional activity by measuring increase in SEAP activity at 1 to 5 uM pretreated for 16 hrs followed by LPS stimulation and measured after 4 hrs by quanti blue reagent-based reporter gene assay | Mus musculus | 1 | assay format | Scientific Literature | ||
14. | ALA4615735 | B | Inhibition of HDAC1 in mouse N2A cells | Mus musculus | 3 | cell-based format | Scientific Literature | ||
15. | ALA5146455 | B | Inhibition of HDAC1 in mouse B16 cells assessed as accumulation of acetylated H3 level at 6 uM after 24 hrs by Western blotting analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
16. | ALA5146456 | B | Inhibition of HDAC1 in mouse B16 cells assessed as accumulation of acetylated H3 level at 2 to 6 uM after 24 hrs by Western blotting analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
17. | ALA5146457 | B | Inhibition of HDAC1 in mouse CT26 cells assessed as accumulation of acetylated H3 level at 6 uM after 24 hrs by Western blotting analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
18. | ALA5146458 | B | Inhibition of HDAC1 in mouse CT26 cells assessed as accumulation of acetylated H3 level at 2 to 6 uM after 24 hrs by Western blotting analysis | Mus musculus | 1 | cell-based format | Scientific Literature |