#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3586822	Binding	Inhibition of Sprague-Dawley rat brain microsomal FAAH using N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide as substrate assessed as 4-pyren-1-ylbutanoic acid release preincubated for 10 mins with substrate followed by protein addition measured after 60 mins by reverse phase HPLC with fluorescence detection method	Rattus norvegicus	23	ALA3585341	assay format	Scientific Literature
2.	ALA3586823	Binding	Inhibition of Sprague-Dawley rat brain microsomal FAAH using N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide as substrate assessed as 4-pyren-1-ylbutanoic acid release at 10 uM preincubated for 10 mins with substrate followed by protein addition measured after 60 mins by reverse phase HPLC with fluorescence detection method	Rattus norvegicus	3	ALA3585341	assay format	Scientific Literature
3.	ALA3705650	Binding	Enzyme Assay: All compound solutions are made to a concentration of 10 mM in DMSO. To test the stability of the compounds in enzyme assay conditions, 25 nmoles of the compound are incubated in TME buffer with 0.1% BSA (final volume of 250 uL) for 15 minutes at 37 C. Samples (100 uL) are taken at the start of the assay and after 15 minutes, diluted 1:5 with acetonitrile and centrifuged (20,000 RCF, five minutes, room temperature) to precipitate the proteins. The resulting supernatant is injected onto the HPLC. Calculations for determining the percent compound remaining are described in the following equation:% R=Peak Area (T15)/Peak Area (T0)To determine whether or not the compounds are good substrates for FAAH, 25 nmoles of the compound were incubated with 75 ug enzyme preparation in TME buffer with 0.1% BSA (final volume 250 uL). The reaction mixture is treated in the same manner as described above.	Rattus norvegicus	59	ALA3638500	single protein format	BindingDB Database
4.	ALA3706256	Binding	Inhibition Assay: FAAH activity was determined by measuring the liberation of the highly fluorescent 7-amino, 4-methyl Coumarin (AMC) generated during hydrolysis of the substrate Arachidonoyl 7-Amino, 4-methyl Coumarin Amide (AAMCA) by FAAH. Inhibition of FAAH activity was determined as a percentage reduction of the fluorescence determined in the absence of compound.The assay was carried out in black-walled, clear bottom, 384-well plates. 27.5 ul of FAAH protein (in FAAH assay buffer: 50 mM Hepes, 0.01% Triton X-100, 1 mM EDTA, 0.5 mg/ml BSA (fatty-acid-free), pH 8.2) was pre-incubated, at 120 nM, with increasing concentrations of compounds (2.5 ul in 100% DMSO) for 0, 1 or 3 hours at room temperature. 2.5 ul of DMSO was added for total controls (100% FAAH activity) and 2.5 ul of URB-597, a known inhibitor of FAAH activity, (at a final, saturating, concentration of 10 uM) was used for non-specific controls (0 FAAH activity).	Rattus norvegicus	4	ALA3638839	single protein format	BindingDB Database
5.	ALA3771523	Binding	Inhibition of rat brain FAAH assessed as hydrolysis of [14C]AEA to [14C]Ethanolamine incubated for 30 mins by scintillation counting method	Rattus norvegicus	36	ALA3769358	assay format	Scientific Literature
6.	ALA3779260	Binding	Inhibition of FAAH in Sprague-Dawley rat brain homogenates preincubated for 10 mins followed by addition of substrate measured after 30 mins by liquid scintillation counting	Rattus norvegicus	40	ALA3774360	tissue-based format	Scientific Literature
7.	ALA3791985	Binding	Inhibition of FAAH in rat brain membranes using using N-arachidonoyl-[14C]-ethanolamine as substrate assessed as reduction in [14C]ethanolamine production incubated for 30 mins by scintillation counting method	Rattus norvegicus	5	ALA3785023	cell membrane format	Scientific Literature
8.	ALA3791996	Binding	Inhibition of FAAH in rat brain membranes up to 500 nM preincubated for 20 mins followed by fluorophosphonate-rhodamine addition measured after 30 mins by competitive affinity based proteomic profiling assay	Rattus norvegicus	1	ALA3785023	cell membrane format	Scientific Literature
9.	ALA3790980	ADME	Drug metabolism in rat liver lysate assessed as FAAH-mediated compound hydrolysis at 5 uM by LC-MS-MS analysis	Rattus norvegicus	2	ALA3785029	tissue-based format	Scientific Literature
10.	ALA3790988	ADME	Drug metabolism in rat liver lysate assessed as FAAH-mediated compound hydrolysis at 5 uM by LC-MS-MS analysis in the presence of PF3845	Rattus norvegicus	2	ALA3785029	tissue-based format	Scientific Literature
11.	ALA3803145	Binding	Inhibition of FAAH in rat brain membrane using anandamide[ethanolamine-3H] as substrate assessed as reduction in [3H]-ethanolamine production incubated for 30 mins by scintillation counting method	Rattus norvegicus	1	ALA3797187	cell membrane format	Scientific Literature
12.	ALA3812746	Binding	Apparent inhibition of recombinant rat FAAH expressed in SK-N-MC cells assessed as [3H]-anandamide hydrolysis after 1 hr by liquid scintillation analysis	Rattus norvegicus	19	ALA3808364	cell-based format	Scientific Literature
13.	ALA3829831	Binding	Inhibition of FAAH in rat brain membrane using N-arachidonoyl-[14C]-ethanolamine as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by scintillation counting method	Rattus norvegicus	24	ALA3826877	cell membrane format	Scientific Literature
14.	ALA3829832	Binding	Inhibition of FAAH in rat brain membrane using N-arachidonoyl-[14C]-ethanolamine as substrate incubated for 30 mins by scintillation counting method	Rattus norvegicus	24	ALA3826877	cell membrane format	Scientific Literature
15.	ALA3829836	Binding	Inhibition of FAAH in rat brain membrane at pH 9 using N-arachidonoyl-[14C]-ethanolamine as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by scintillation counting method	Rattus norvegicus	2	ALA3826877	cell membrane format	Scientific Literature
16.	ALA3829837	Binding	Inhibition of FAAH in rat brain membrane at pH 7.4 using N-arachidonoyl-[14C]-ethanolamine as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by scintillation counting method	Rattus norvegicus	2	ALA3826877	cell membrane format	Scientific Literature
17.	ALA3829838	Binding	Ratio of IC50 at pH7.4 to pH 9 for FAAH in rat brain membrane	Rattus norvegicus	2	ALA3826877	cell membrane format	Scientific Literature
18.	ALA857284	Binding	Km value for hydrolysis by rat brain microsomal anandamide amidohydrolase (AAH).		5	ALA1132600	assay format	Scientific Literature
19.	ALA649403	Binding	The compound was tested for hydrolysis by rat brain microsomal anandamide amidohydrolase (AAH) in the presence of (-)-11-OH-DMH-delta-8-THC (30 uM).		1	ALA1132600	assay format	Scientific Literature
20.	ALA649404	Binding	The compound was tested for hydrolysis by rat brain microsomal anandamide amidohydrolase (AAH) in the presence of (-)-delta8-THC (30 uM).		1	ALA1132600	assay format	Scientific Literature