#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA615272	Binding	Inhibitory activity against 5,10-methylenetetrahydrofolate dehydrogenase from Escherichia coli at a concentration of 0.1 mM; NT=Not tested	Escherichia coli	1	ALA1125043	single protein format	Scientific Literature
2.	ALA615103	Binding	Inhibitory activity against 5,10-Methylene Tetrahydrofolate Cyclohydrolase from Escherichia coli at a concentration of 0.1 mM; NT=Not tested	Escherichia coli	1	ALA1125043	single protein format	Scientific Literature
3.	ALA615104	Binding	Inhibitory activity against 5,10-Methylene Tetrahydrofolate Cyclohydrolase from Escherichia coli at a concentration of 0.1 mM;NT=Not tested	Escherichia coli	2	ALA1125043	single protein format	Scientific Literature
4.	ALA615106	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (L1210 tumor tissue) enzyme at 10E-6 M inhibitory concentration	Sus scrofa	1	ALA1122152	single protein format	Scientific Literature
5.	ALA615107	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (A,porcine liver) enzyme.	Sus scrofa	1	ALA1122152	assay format	Scientific Literature
6.	ALA615108	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (L1210 tumor tissue) enzyme at 10E-4 M inhibitory concentration	Sus scrofa	2	ALA1122152	single protein format	Scientific Literature
7.	ALA615109	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (L1210 tumor tissue) enzyme at 10E-5 M inhibitory concentration	Sus scrofa	1	ALA1122152	single protein format	Scientific Literature
8.	ALA615110	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (L1210 tumor tissue) enzyme at 10E-6 M inhibitory concentration	Sus scrofa	1	ALA1122152	single protein format	Scientific Literature
9.	ALA840105	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (porcine liver) enzyme at 10E-4 M inhibitory concentration	Sus scrofa	2	ALA1122152	assay format	Scientific Literature
10.	ALA615111	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (porcine liver) enzyme at 10E-5 M inhibitory concentration	Sus scrofa	1	ALA1122152	assay format	Scientific Literature
11.	ALA615112	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (porcine liver) enzyme at 10E-6 M inhibitory concentration	Sus scrofa	2	ALA1122152	assay format	Scientific Literature
12.	ALA615113	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (porcine liver) enzyme at 10E-7 M inhibitory concentration	Sus scrofa	1	ALA1122152	assay format	Scientific Literature
13.	ALA615114	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (porcine liver) enzyme.	Sus scrofa	1	ALA1122152	assay format	Scientific Literature
14.	ALA615115	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (L1210 tumor tissue) enzyme at 10E-6 M inhibitory concentration	Sus scrofa	1	ALA1122152	single protein format	Scientific Literature
15.	ALA615116	Binding	Percent inhibition was measured against 5,10-Methylene Tetrahydrofolate Cyclohydrolase (porcine liver) enzyme at 10E-4 M inhibitory concentration	Sus scrofa	1	ALA1122152	assay format	Scientific Literature
16.	ALA615698	Binding	Compound was tested for in vitro inhibition of 5,10-Methylene Tetrahydrofolate Cyclohydrolase, competitive against (+)-L-5,10-methenyltetrahydrofolate		1	ALA1121915	single protein format	Scientific Literature
17.	ALA4022807	Binding	Inhibition of MTHFD1 (unknown origin) using NADP/5,10-methylene THF as substrate after 30 mins by HPLC method	Homo sapiens	1	ALA4020815	single protein format	Scientific Literature
18.	ALA4338135	Binding	Inhibition of recombinant MTHFD1 (unknown origin) using THF as substrate incubated for 30 mins in presence of NAD	Homo sapiens	23	ALA4334513	single protein format	Scientific Literature
19.	ALA4434261	Binding	Inhibition of His-tagged human MTHFD1 ( 1 to 306 residues) expressed in Escherichia coli BL21 (DE3) pre-incubated for 10 mins before folitixorin and NADP+ addition measured after 15 mins by NAD(P)H-Glo detection reagent based luminescence assay	Homo sapiens	1	ALA4433244	assay format	Scientific Literature
20.	ALA4434263	Binding	Inhibition of recombinant MTHFD1 (unknown origin) assessed as reduction in methenyl-THF formation using tetrahydrofolate, NADP and formaldehyde incubated for 30 mins by NADP-dependent dehydrogenase assay	Homo sapiens	11	ALA4433244	single protein format	Scientific Literature