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# Aladdin ID Assay Type Description Organism Compounds Reference BAO Format Source 1. ALA860111 B Inhibition of canine retinal cone PDE6 Canis lupus familiaris 5 single protein format Scientific Literature 2. ALA998836 B Inhibition of human PDE6C at 10 uM Homo sapiens 6 single protein format Scientific Literature 3. ALA998838 B Inhibition of human PDE6C Homo sapiens 2 single protein format Scientific Literature 4. ALA2184506 B Inhibition of human recombinant PDE6C at 1 uM by [3H]cGMP based tritium scintillation proximity assay Homo sapiens 2 single protein format Scientific Literature 5. ALA3405817 B Inhibition of PDE6C (unknown origin) Homo sapiens 2 single protein format Scientific Literature 6. ALA3631029 B Inhibition of human PDE6C using AM-Cyclic-3',5'-AMP at 10 uM after 60 mins by fluorescence polarization assay Homo sapiens 1 single protein format Scientific Literature 7. ALA3631049 B Inhibition of human PDE6C using AM-Cyclic-3',5'-AMP after 60 mins by fluorescence polarization assay Homo sapiens 1 single protein format Scientific Literature 8. ALA3861027 B Inhibition of recombinant N-terminal GST tagged PDE6C (unknown origin) expressed in baculovirus infected Sf9 cells using cGMP as substrate by malachite green reagent based assay Homo sapiens 2 cell-based format Scientific Literature 9. ALA3875441 B Inhibition of human PDE6C using FAM-3',5'-cAMP as substrate after 1 hr by fluorescence polarization assay Homo sapiens 5 single protein format Scientific Literature 10. ALA3875457 B Inhibition of human PDE6C at 10'4 nM using FAM-3',5'-cAMP as substrate after 1 hr by fluorescence polarization assay relative to control Homo sapiens 4 single protein format Scientific Literature 11. ALA3887442 B In Vitro Enzyme Assay: In vitro enzyme assays were conducted via the Ba(OH)2 precipitation method of Wang et al. (32) using recombinant human PDE11A4, PDE10A1, PDE5A1, PDE6C, PDE8A (BPS Bioscience Inc.), PDE7A (BIOMOL International), and PDE4A10 enzymes (gift from HengmingKe), in the presence of 100 nM cGMP, 30 nM cAMP, 500 nM cGMP, 1.7 μM cGMP, 10 nM cAMP, 15 nM cAMP, and 625 nM cAMP, respectively. Inhibitor concentrations that reduce enzyme activity by 50% (IC50) are presented. The values are means of at least three independent experiments. 8 single protein format BindingDB Database 12. ALA4008524 B Inhibition of PDE6C (unknown origin) expressed in Baculovirus infected Sf9 insect cells using cGMP as substrate Homo sapiens 1 cell-based format Scientific Literature 13. ALA4011527 B Inhibition of N-terminal GST-tagged full length recombinant human PDE6C expressed in Baculovirus infected Sf9 insect cells Homo sapiens 2 cell-based format Scientific Literature 14. ALA4020496 B Inhibition of human recombinant PDE6C using FAM-labelled cGMP as substrate after 60 mins by fluorescence polarization assay Homo sapiens 18 single protein format Scientific Literature 15. ALA4047071 A Inhibition of human PDE6C at 100 uM Homo sapiens 1 single protein format Scientific Literature 16. ALA4155491 B Inhibition of PDE6C (unknown origin) at 100 nM using iFl-cAMP/iFl-cGMP as substrate by mobility shift assay relative to control Homo sapiens 2 single protein format Scientific Literature 17. ALA4189131 B Inhibition of human PDE6C at 50 uM relative to control Homo sapiens 2 single protein format Scientific Literature 18. ALA4189147 B Inhibition of human PDE6C Homo sapiens 2 single protein format Scientific Literature 19. ALA4198222 B Inhibition of N-terminal GST-tagged full length recombinant human PDE6C expressed in Baculovirus infected Sf9 insect cells using FAM-cAMP as substrate after 1 hr by fluorescence polarization assay Homo sapiens 8 cell-based format Scientific Literature 20. ALA4198940 B Inhibition of PDE6C (unknown origin) using FAM-cGMP as substrate by fluorescence assay Homo sapiens 1 single protein format Scientific Literature