| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2166287 | B | Activation of human hexokinase 2 at 500 times EC50 concentration | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 2. | ALA2318450 | B | Induction of hexokinase 2 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 3. | ALA3386522 | B | Inhibition of hexokinase 2 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 4. | ALA3578774 | B | Effect on hexokinase-2 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 5. | ALA3807034 | B | Inhibition of His-tagged human HK2 (17 to 916 residues) expressed in Escherichia coli BL21(DE3) using glucose as substrate after 45 mins by ADP-glo assay in presence of MgATP | Homo sapiens | 25 | assay format | Scientific Literature | ||
| 6. | ALA3807036 | B | Inhibition of HK2 in human UM-UC-3 cells using 2FDG as substrate assessed as suppression of G6P production preincubated for 30 mins followed by substrate treatment for 90 mins by resazurin assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 7. | ALA3807037 | B | Inhibition of HK2 in human UM-UC-3 cells assessed as reduction in glucose consumption incubated up to 20 hrs | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA3807038 | B | Inhibition of HK2 in human UM-UC-3 cells assessed as production of [3-13C]-lactate from D-[1,6-13C2]glucose at 10 uM preincubated for 1 hr followed by D-[1,6-13C2]glucose addition measured every 10 mins by real-time 13C-NMR spectroscopic analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA3807039 | B | Competitive inhibition of His-tagged human HK2 (17 to 916 residues) expressed in Escherichia coli BL21(DE3) assessed as formation of G6P by continuous G6P dehydrogenase coupled assay in presence of glucose | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 10. | ALA3807040 | B | Non-competitive inhibition of His-tagged human HK2 (17 to 916 residues) expressed in Escherichia coli BL21(DE3) using glucose as substrate assessed as formation of G6P at 1.25 to 20 nM by continuous G6P dehydrogenase coupled assay in presence of MgATP | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA3807228 | B | Inhibition of His-tagged human HK2 (17 to 916 residues) expressed in Escherichia coli BL21(DE3) assessed as ADP formation at 1.25 to 10 nM by pyruvate kinase/lactate dehydrogenase coupled assay in presence of G6P | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA4003886 | B | Inhibition of recombinant human HK2 using [U-14C]-labeled glucose as substrate in presence of ATP and NADP by G6PD enzyme coupled spectrophotometric assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 13. | ALA4321597 | B | Inhibition of HK2 (unknown origin) using glucose-6-phosphate dehydrogenase as substrate preincubated for 10 mins followed by substrate addition | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 14. | ALA4321598 | B | Competitive-inhibition of HK2 (unknown origin) assessed as Km at 10 uM using varying levels of glucose-6-phosphate dehydrogenase as substrate by Lineweaver-burk plot analysis | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 15. | ALA4349319 | B | Inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) using glucose as substrate preincubated for 10 mins followed by substrate addition in presence of ATP by G6PDH/NADP coupled assay | Homo sapiens | 21 | assay format | Scientific Literature | ||
| 16. | ALA4349320 | B | Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Vmax using 3.125 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.019 OD/min) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 17. | ALA4349321 | B | Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Vmax using 6.25 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.019 OD/min) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 18. | ALA4349322 | B | Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Vmax using 12.5 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.019 OD/min) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 19. | ALA4349323 | B | Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Km using 3.125 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 1.62 mM) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 20. | ALA4349324 | B | Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Km using 6.25 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 1.62 mM) | Homo sapiens | 1 | assay format | Scientific Literature |
The page will load shortly, Thanks for your patience!