# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4118765 | B | Binding affinity to RTN4 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | ALA4118021 | cell-based format | Scientific Literature | |
2. | ALA4118910 | B | Binding affinity to RTN4 in human NCI-H358 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | ALA4118021 | cell-based format | Scientific Literature | |
3. | ALA4341382 | B | Binding affinity to RTN4 in human A549 cells lysates grown on SILAC media at 10 uM incubated for 1 hr by LC-MS/MS analysis | Homo sapiens | 3 | ALA4340528 | subcellular format | Scientific Literature | |
4. | ALA4341397 | B | Binding affinity to RTN4 in human A549 cells lysates grown on SILAC media at 10 uM incubated for 1 hr by LC-MS/MS analysis relative to untreated control | Homo sapiens | 2 | ALA4340528 | subcellular format | Scientific Literature | |
5. | ALA4721798 | B | Covalent binding affinity to RTN4 at Cys1101 residue in human NCI-H358 cells assessed as log2 H/L ratio at 10 uM pretreated for 3 hrs followed by N-(((6S,7R)-7-(4-chloro-3-fluorophenyl)-1,4-oxazepan-6-yl)methyl)-2-hydroxyacetamide treatment for 3 hrs by competitive TMT click chemistry pull-down experiment based LC-MS/MS analysis | Homo sapiens | 1 | ALA4715824 | cell-based format | Scientific Literature | |
6. | ALA4721805 | B | Covalent binding affinity to RTN4 at Cys1101 residue in human NCI-H358 cells assessed as reduction of log2 H/L ratio for tryptic peptide YSNSALGHVNCTIK incubated for 4 hrs followed by cell lysis and subsequently labelled with light thiol probe N-((S)-18-iodo-11-isopropyl-10,13,17-trioxo-3,6-dioxa-9,12,16-triazaoctadecyl)-6-((4R,5S)-5-methyl-2-oxoimidazolidin-4-yl)hexanamide and heavy thiol probe 4 for 1 hr under dark conditions followed by tryptic digestion for overnight and measured by discovery ABPP based nano LC-MS/MS analysis | Homo sapiens | 1 | ALA4715824 | cell-based format | Scientific Literature |