# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA644748 | B | Inhibition against membrane bound monkey aminopeptidase P | Cercopithecidae | 13 | assay format | Scientific Literature | ||
2. | ALA644753 | B | Inhibition against membrane bound human aminopeptidase P | 13 | assay format | Scientific Literature | |||
3. | ALA1821051 | B | Inhibition of aminopeptidase P | 1 | single protein format | Scientific Literature | |||
4. | ALA3091132 | A | Stability of the compound assessed as recombinant human APP2-mediated compound degradation at 0.1 mM after 1 hr by reverse-phase HPLC/MS analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
5. | ALA3091133 | A | Stability of the compound assessed as half life of recombinant human APP2-mediated compound degradation at 0.1 mM by reverse-phase HPLC/MS analysis | Homo sapiens | 3 | single protein format | Scientific Literature | ||
6. | ALA3887729 | B | Enzyme Assay: Human aminopeptidase P2 (APP2) enzyme activity was determined using the internally quenched fluorescent substrate: H-Lys(2-aminobenzoyl)-Pro-Pro-p-nitroanilide (Bachem, Torrance, Calif.) at 1 μM (=Km) for inhibitor studies and 5 μM for enzyme purification assays. The assay buffer was 0.1 M HEPES, pH 7.4, and the assay temperature was 30° C. Cleavage of the substrate by the enzyme produces an increase in fluorescence due to the release of H-Lys(2-aminobenzoyl). Enzyme rates were determined in an F. fluorescence plate reader (Molecular Devices, Sunnyvale, Calif.) using an excitation wavelength of 320 nm and an emission wavelength of 405 nm. | 4 | single protein format | BindingDB Database |