| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA765611 | B | Inhibitory activity against Protein tyrosine phosphatase PTPmeg-1. | 1 | single protein format | Scientific Literature | |||
| 2. | ALA765612 | B | Inhibitory activity against Protein tyrosine phosphatase PTPmeg-1 at 250 uM | 1 | single protein format | Scientific Literature | |||
| 3. | ALA765613 | B | Inhibitory activity against Protein tyrosine phosphatase PTPmeg-1 at 250 uM | 1 | single protein format | Scientific Literature | |||
| 4. | ALA765614 | B | Inhibitory activity against Protein tyrosine phosphatase PTPmeg-1 at 380 uM | 1 | single protein format | Scientific Literature | |||
| 5. | ALA765615 | B | Inhibitory activity against Protein tyrosine phosphatase PTPmeg-1 at 500 uM | 1 | single protein format | Scientific Literature | |||
| 6. | ALA3888466 | B | Enzyme Kinetic Assay: PTP activity was assayed using p-nitrophenyl phosphate (pNPP) as a substrate in 3,3-dimethylglutarate buffer (50 mM 3,3-dimethylglutarate, pH 7.0, 1 mM EDTA, 150 mM NaCl, 2 mM DTT, 0.1 mg/mL BSA) at 25° C. The assays were performed in 96-well plates. Normally, to determine the IC50 values, the reaction was initiated by the addition of enzyme (final concentration at 10 nM) to a reaction mixture (0.2 mL) containing 2 mM (Km for the substrate) pNPP with various concentrations of inhibitors. The reaction rate was measured using a SpectraMax Plus 384 Microplate Spectrophotometer (Molecular Devices). To determine the mode of inhibition, the reactions were initiated by the addition of PTP-MEG2 to the reaction mixtures (0.2 mL) containing various concentrations of pNPP with different concentrations of the inhibitor 7. | Homo sapiens | 1 | single protein format | BindingDB Database | ||
| 7. | ALA4424213 | B | Inhibition of recombinant human N-terminal GST-tagged MEG1 catalytic domain (637 to 926 residues) expressed in Escherichia coli using p-nitrophenol as substrate preincubated with substrate for 5 mins followed by enzyme addition | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 8. | ALA4434593 | B | Substrate activity at MEG1 (unknown origin) expressed in Escherichia coli BL21 (DE3) incubated for 60 mins by fluorescence based assay | Homo sapiens | 2 | assay format | Scientific Literature |
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