| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA752719 | B | In vitro inhibitory activity against human Jurkat T-Cells stably transfected with an Nuclear factor kappa B transcription factor | 31 | cell-based format | Scientific Literature | |||
| 2. | ALA832189 | B | inhibitory effect on the DNA binding of Nuclear factor kappa B; Active | 1 | single protein format | Scientific Literature | |||
| 3. | ALA2114853 | F | PubChem BioAssay. qHTS assay for small molecule activators of the nuclear factor-kappa B (NF-kB) signaling pathway. (Class of assay: confirmatory) | Homo sapiens | 180 | assay format | PubChem BioAssays | ||
| 4. | ALA977405 | B | Inhibition of DNA binding activity of wild type NF-kappaB p50 | 1 | single protein format | Scientific Literature | |||
| 5. | ALA977407 | B | Inhibition of DNA binding activity of wild type NF-kappaB p50 by MALDI-TOF MS analysis | 1 | single protein format | Scientific Literature | |||
| 6. | ALA1041917 | B | Inhibition of GST-fused human NF-kappaB p50 subunit DNA binding activity assessed as shortened diffusion time by fluorescence correlation spectroscopy | Homo sapiens | 7 | single protein format | Scientific Literature | ||
| 7. | ALA1041918 | B | Inhibition of GST-fused human NF-kappaB p50 subunit DNA binding activity assessed as shortened diffusion time by electrophoretic mobility shift assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 8. | ALA1100041 | B | Inhibition of p50 after 1 hr by ELISA | 12 | single protein format | Scientific Literature | |||
| 9. | ALA1108521 | B | Inhibition of NF kappaB p50 subunit signaling pathway in human HeLa cells by biotinylated-ELISA | Homo sapiens | 9 | cell-based format | Scientific Literature | ||
| 10. | ALA1109181 | B | Inhibition of NFkappa p50 isolated from nuclear extract of human HeLa cells assessed as blockade of binding to biotinylated consesus sequence by chemiluminescence assay | Homo sapiens | 15 | cell-based format | Scientific Literature | ||
| 11. | ALA1291967 | B | Inhibition of NF-KB p50 subunit/DNA interaction after 20 mins by EMSA | 11 | single protein format | Scientific Literature | |||
| 12. | ALA1614274 | F | PUBCHEM_BIOASSAY: qHTS Assay for Identification of Small Molecule Antagonists for NFkB Signaling Pathway. (Class of assay: confirmatory) | Homo sapiens | 518 | assay format | PubChem BioAssays | ||
| 13. | ALA1613823 | F | PUBCHEM_BIOASSAY: qHTS Assay for Identification of Small Molecule Agonists for NFkB Signaling Pathway. (Class of assay: confirmatory) [Related pubchem assays: 895 ] | Homo sapiens | 518 | assay format | PubChem BioAssays | ||
| 14. | ALA2330627 | B | Inhibition of human NF-kappaB p50/DNA interaction using 32P-labeled oligonucleotides as substrate preincubated for 20 mins before substrate addition measured after 20 mins by electrophoretic mobility shift assay | Homo sapiens | 58 | single protein format | Scientific Literature | ||
| 15. | ALA3269915 | B | Inhibition of NF-kappaB p50 subunit translocation from cytoplasm to nucleus in TNF-alpha-stimulated human Jurkat cells at 40 uM preincubated for 2 hrs followed by TNF-alpha challenge measured after 2 to 90 mins by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA3428782 | B | Binding affinity to 6His-tagged recombinant human NF-kappaB p50 N-terminal domain expressed in Escherichia coli BL21 (DE3) up to 50:1 compound to protein molar ratio after 2 hrs by mass spectrometric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 17. | ALA3428783 | B | Binding affinity to 6His-tagged recombinant human NF-kappaB p50 N-terminal domain expressed in Escherichia coli BL21 (DE3) at 4:1 compound to protein molar ratio after 2 hrs by mass spectrometric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 18. | ALA3428784 | B | Binding affinity to 6His-tagged recombinant human NF-kappaB p50 N-terminal domain expressed in Escherichia coli BL21 (DE3) up to 10:1 compound to protein molar ratio after 2 hrs by mass spectrometric analysis | Homo sapiens | 5 | assay format | Scientific Literature | ||
| 19. | ALA3428785 | B | Binding affinity to 6His-tagged recombinant human NF-kappaB p50 N-terminal domain expressed in Escherichia coli BL21 (DE3) after 2 hrs by mass spectrometric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 20. | ALA3428786 | B | Binding affinity to 6His-tagged recombinant human NF-kappaB p50 N-terminal domain expressed in Escherichia coli BL21 (DE3) at 20:1 compound to protein molar ratio after 2 hrs by mass spectrometric analysis in presence of DTT | Homo sapiens | 1 | assay format | Scientific Literature |
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