#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3619925	Binding	Inhibition of fatty acid synthase (unknown origin) by scintillation proximity assay	Homo sapiens	6	ALA3616391	single protein format	Scientific Literature
2.	ALA3619926	Binding	Inhibition of fatty acid synthase keto-reductase domain (unknown origin)	Homo sapiens	4	ALA3616391	single protein format	Scientific Literature
3.	ALA3625454	Binding	Inhibition of fatty acid synthase KE domain (unknown origin)	Homo sapiens	1	ALA3621185	single protein format	Scientific Literature
4.	ALA3625457	Binding	Inhibition of fatty acid synthase (unknown origin)	Homo sapiens	6	ALA3621185	single protein format	Scientific Literature
5.	ALA3625458	Binding	Inhibition of fatty acid synthase KR domain (unknown origin)	Homo sapiens	2	ALA3621185	single protein format	Scientific Literature
6.	ALA3705868	Binding	Biochemical Assay: The FASN enzyme was isolated from SKBr3 cells. SKBr3 is a human breast cancer cell-line with high levels of FASN expression. It is estimated that FASN comprises about 25% of the cytosolic proteins in this cell line. SKBr3 cells were homogenized in a dounce homogenizer then centrifuged for 15 minutes at 4C. to remove particulate matter. The supernatant was then analyzed for protein content, diluted to the appropriate concentration, and used to measure FASN activity. The presence of FASN was confirmed by western blot analysis. A similar method for isolation of FASN from SKBr3 cells is described in Teresa, P. et al. (Clin. Cancer Res. 2009; 15(24), 7608-7615).FASN activity of the SKBr3 cell extract was determined by measuring either NADPH oxidation or the amount of thiol-containing coenzyme A (CoA) released during the fatty acid synthase reaction. The dye CPM (7-diethylamino-3-(4â-maleimidyl-phenyl)-4-methylcoumarin) contains a thiol reactive group that increases its fluorescence emission.		472	ALA3638966	cell-based format	BindingDB Database
7.	ALA3706007	Binding	Biological Assay: Assay #1:Inhibition of FAS activity can be measured based on the detection of residual NADPH substrate after the FAS assay is quenched. This assay is run as a 10 uL endpoint assay in 384-well format, where the reaction contains 20 uM malonyl-CoA, 2 uM acetyl-CoA, 30 uM NADPH and 40 nM FAS in 50 mM sodium phosphate, pH 7.0. The assay is run by sequentially dispensing 5 ul of a malonyl-CoA solution, then enzyme solution (containing the acetyl-CoA, and NADPH) into a black, low volume assay plate (Greiner 784076) pre-dispensed with 100 nL compound solutions in DMSO. The reaction is incubated at ambient temperature for 60 minutes, then quenched with 5 uL of a developing solution composed of 90 uM resazurin, 0.3 IU/ml diaphorase in 50 mM sodium phosphate, pH 7.0. The developed reaction is read on a Molecular Devices Analyst or Acquest (or equivalent) plate reader using a 530 nm excitation wavelength filter, a 580 nm emission filter, and 561 nm dichroic filter.		12	ALA3638836	single protein format	BindingDB Database
8.	ALA682484	Binding	Inhibition constant against Escherichia coli cyclopropane fatty acid synthase	Escherichia coli	10	ALA1148231	single protein format	Scientific Literature
9.	ALA873666	Binding	Inhibition of fatty acid synthase at a concentration of 150 (uM)		33	ALA1132487	single protein format	Scientific Literature
10.	ALA828726	Binding	Inhibitory concentration against purified human fatty acid synthase activity in ZR-75-1 breast cancer cells	Homo sapiens	38	ALA1142684	cell-based format	Scientific Literature
11.	ALA858886	Binding	Inhibitory activity against human FAS1 at 100 uM	Homo sapiens	5	ALA1148604	single protein format	Scientific Literature
12.	ALA863392	Binding	Inhibitory activity against human FAS1	Homo sapiens	2	ALA1148604	single protein format	Scientific Literature
13.	ALA862461	Binding	Inhibition of FAS	Homo sapiens	14	ALA1149347	single protein format	Scientific Literature
14.	ALA862462	Binding	Inhibition of FAS at 100 uM	Homo sapiens	2	ALA1149347	single protein format	Scientific Literature
15.	ALA869274	Binding	Inhibition of human FAS	Homo sapiens	25	ALA1149512	single protein format	Scientific Literature
16.	ALA1260142	Binding	Inhibition of FAS		13	ALA1255179	single protein format	Scientific Literature
17.	ALA1260143	Binding	Inhibition of FAS at 1000 uM		1	ALA1255179	single protein format	Scientific Literature
18.	ALA1839595	Binding	Inhibition of human FAS assessed as synthesis of long chain fatty acids from malonyl CoA after 60 mins by fluorescence assay	Homo sapiens	25	ALA1833814	single protein format	Scientific Literature
19.	ALA3411198	Binding	Inhibition of purified recombinant FASN TE activity (unknown origin) using 4-MUH as substrate preincubated for 30 mins before substrate addition measured after 1 hr by fluorescence assay	Homo sapiens	6	ALA3407413	single protein format	Scientific Literature
20.	ALA3411205	Binding	Inhibition of FASN in human PANC1 cells assessed as inhibition of [14C]acetate incorporation preincubated for 4 hrs before [14C]acetate addition measured after 2 hrs by scintillation counting	Homo sapiens	2	ALA3407413	cell-based format	Scientific Literature