# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA760888 | B | Inhibition of Progesterone 21-hydroxylase cytochrome P450 21 | Rattus norvegicus | 3 | single protein format | Scientific Literature | ||
2. | ALA3583945 | B | Inhibition of human CYP21 expressed in COS7 cells incubated for 1 hr before 17-hydroxypregnenolone substrate addition by HTRF-based assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
3. | ALA3784034 | A | Inhibition of CYP21A2 in human H295R cells using hydroxy progesterone as substrate incubated for 120 mins by LC-MS method | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
4. | ALA3868074 | B | Inhibition of recombinant CYP21 (unknown origin) overexpressed in human AD293 cells assessed as reduction in 11-deoxycortisol formation preincubated for 60 mins followed by addition of 17-alpha-hydroxyprogesterone as substrate measured after 45 mins by LC-MS/MS analysis | Homo sapiens | 13 | cell-based format | Scientific Literature | ||
5. | ALA3872121 | B | Inhibition of human CYP21 expressed in African green monkey COS7 cells using 17-hydroxypregnenolone as substrate preincubated for 1 hr followed by substrate addition after 3 hrs by HTRF assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
6. | ALA3882751 | B | Inhibition of human CYP21 transfected in HEK293 cells assessed as decrease in conversion of progesterone to 11-deoxycorticosterone at 100 nM preincubated for 5 hrs followed by progesterone addition measured after 20 hrs by ELISA relative to control | Homo sapiens | 407 | cell-based format | Patent Bioactivity Data | ||
7. | ALA3882753 | B | Inhibition of human CYP21 transfected in HEK293 cells assessed as decrease in conversion of progesterone to 11-deoxycorticosterone preincubated for 5 hrs followed by progesterone addition measured after 20 hrs by ELISA | Homo sapiens | 290 | cell-based format | Patent Bioactivity Data | ||
8. | ALA4029978 | B | Inhibition of human CYP21 expressed in African green monkey COS7 cells using 17-hydroxypregnenolone as substrate preincubated for 1 hr followed by substrate addition measured for 3 hrs by HTRF assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
9. | ALA4190137 | A | Inhibition of C-terminal His-tagged recombinant human CYP21A2deltaH mutant expressed in Escherichia coli DH5alpha assessed as decrease in progesterone hydroxylation preincubated for 3 mins followed by NADPH addition measured after 10 mins in presence of cytochrome p450 reductase by LC-MS/MS method | Homo sapiens | 11 | assay format | Scientific Literature | ||
10. | ALA4256958 | A | Inhibition of human membrane bound C-terminal His4-tagged CYP21A2 delta19H mutant assessed as reduction in progesterone hydroxylation measured after 10 mins in presence of NADPH/NADPH-cytochrome p450 reductase by GC/MS method | Homo sapiens | 7 | assay format | Patent Bioactivity Data | ||
11. | ALA4257178 | B | Inhibition of recombinant CYP21 (unknown origin) expressed in human AD293 cells using 17alpha-hydroxyprogesterone as substrate pretreated for 60 mins followed by substrate addition and measured after 45 mins by LC/MS analysis | Homo sapiens | 62 | cell-based format | Patent Bioactivity Data | ||
12. | ALA4257192 | B | Inhibition of recombinant CYP21 (unknown origin) expressed in human AD293 cells assessed as reduction in 11-deoxycortisol level preincubated for 60 mins followed by 17alpha-OH-progesterone substrate addition and measured after 45 mins by LC-MS method | Homo sapiens | 23 | cell-based format | Patent Bioactivity Data | ||
13. | ALA5224639 | A | Inhibition of human CYP21A2 | Homo sapiens | 1 | single protein format | Scientific Literature |