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# Aladdin ID Assay Type Description Organism Compounds Reference BAO Format Source 1. ALA744608 B Tested for inhibitory activity against porcine erythrocyte Mu-calpain 7 ALA1135857 single protein format Scientific Literature 2. ALA864541 B Inhibitory activity against porcine calpain I using Suc-Leu-Tyr-AMC fluorogenic substrate Sus scrofa 50 ALA1141207 single protein format Scientific Literature 3. ALA863791 B Inhibition of mu calpain in porcine erythrocyte Sus scrofa 13 ALA1146935 single protein format Scientific Literature 4. ALA869269 B Inhibition of porcine erythrocyte mu calpain Sus scrofa 21 ALA1146033 single protein format Scientific Literature 5. ALA1012289 B Inhibition of pig CAPN1 Sus scrofa 1 ALA1155054 single protein format Scientific Literature 6. ALA989262 B Inhibition of pig erythrocyte mu-calpain by fluorometric assay Sus scrofa 11 ALA1155134 single protein format Scientific Literature 7. ALA980942 B Inhibition of pig erythrocyte mu-calpain Sus scrofa 3 ALA1139408 single protein format Scientific Literature 8. ALA1247359 B Inhibition of porcine erythrocyte calpain 1 Sus scrofa 11 ALA1240500 single protein format Scientific Literature 9. ALA1247363 B Ratio of compound Ki to AK295 Ki for porcine erythrocyte calpain 1 Sus scrofa 4 ALA1240500 single protein format Scientific Literature 10. ALA2411622 B Inhibition of pig full length Cal1 after using SucLLVYAMC as substrate by FRET assay relative to E64 Sus scrofa 5 ALA2407090 single protein format Scientific Literature 11. ALA2411624 B Inhibition of pig full length Cal1 after using SucLLVYAMC as substrate by FRET assay Sus scrofa 5 ALA2407090 single protein format Scientific Literature 12. ALA3224718 B Inhibition of Ca2+-dependent activation of porcine calpain-1 using SucLY-AMC as substrate Sus scrofa 27 ALA3217751 single protein format Scientific Literature 13. ALA3224719 B Inhibition of Ca2+-dependent activation of porcine calpain-1 using H-K(FAM)-EVYGMMK(DABCYL)-OH as substrate by FRET assay Sus scrofa 27 ALA3217751 single protein format Scientific Literature 14. ALA3888844 B Inhibition Kinetics Assay: Full length porcine calpain (156 nM), or papain (236 pM) was added to a solution of 100 mM NaCl, 50 mM HEPES, pH 7.6, 1 mM TCEP, 30 μM Suc-LLVY-AMC (SEQ ID NO: 8) substrate, and inhibitor (0.5 to 50 μM). Calpain reactions also contained CaCl2 (1 mM and 100 mM for porcine and rat respectively). Both substrate and inhibitors were dissolved in acetonitrile/DMSO (1:1) with the exception of E-64, dissolved in water. Organic solvent remained <2% in all reactions, and most often <1%. Reactions were carried out in microtiter 96-well plates, with 150 μL per well, 30° C., and product formation was monitored over time by fluorescence (Ex/Em 346/444 nm, with 420 nm cutoff filter). Kinetic values of kobs were determined via non-linear regression using one-phase association analysis and linear plots of 1/kobs vs. 1/[I] provided kinetic constants ki and KI. Sus scrofa 5 ALA3886857 single protein format BindingDB Database 15. ALA4263663 B Inhibition of full length porcine Calpain-1 complex using (H2NeK(-FAM)-EVYGMMK(DABCYL)eOH) substrate by FRET assay Sus scrofa 11 ALA4261589 single protein format Scientific Literature 16. ALA4263664 B Inhibition of porcine Calpain-1 active site domain using (H2NeK(-FAM)-EVYGMMK(DABCYL)eOH) substrate by FRET assay Sus scrofa 11 ALA4261589 single protein format Scientific Literature 17. ALA4623091 B Inhibition of mu-calpain in porcine erythrocyte using Suc-Leu-Tyr-AMC as substrate by microtiter plate assay based Dixon plot analysis Sus scrofa 7 ALA4622834 cell-based format Scientific Literature