#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA680678	B	In vitro inhibition of Fas-associated phosphatase-1 binding from HFAP-10cDNA subcloned into bluescript vector	Homo sapiens	11		single protein format	Scientific Literature
2.	ALA680036	B	Inhibitory concentration against FAP-1 pNPP		7		single protein format	Scientific Literature
3.	ALA680037	B	Inhibitory concentration against FAP-1 using lck-10 mer as substrate		7		single protein format	Scientific Literature
4.	ALA680038	B	Inhibitory concentration against FAP-1 using lck-10mer as substrate		1		single protein format	Scientific Literature
5.	ALA680039	B	Inhibitory concentration against FAP-1 using pNPP as substrate		1		single protein format	Scientific Literature
6.	ALA925805	B	Inhibition of FAP1		1		single protein format	Scientific Literature
7.	ALA968353	B	Inhibition of protein tyrosine phosphatase N13 phosphorylation		1		single protein format	Scientific Literature
8.	ALA1070017	B	Inhibition of FAP1 expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry		1		assay format	Scientific Literature
9.	ALA1775821	B	Inhibition of human FAP1 expressed in Escherichia coli after 5 mins by microplate spectrophotometer analysis	Homo sapiens	3		assay format	Scientific Literature
10.	ALA2321066	B	Inhibition of FAP1 (unknown origin) expressed in Escherichia coli using pNPP substrate after 5 mins by spectrophotometric analysis	Homo sapiens	3		assay format	Scientific Literature
11.	ALA2401196	B	Inhibition of recombinant FAP-1 (unknown origin) using pNPP as substrate by spectrophotometric analysis	Homo sapiens	2		single protein format	Scientific Literature
12.	ALA3772188	B	Inhibition of FAP1 (unknown origin) expressed in Escherichia coli using pNPP as substrate at 200 uM after 5 mins by spectrophotometry	Homo sapiens	1		assay format	Scientific Literature
13.	ALA3862257	B	Inhibition of recombinant FAP1 (unknown origin) using pNPP as substrate by spectrophotometric method	Homo sapiens	25		single protein format	Scientific Literature
14.	ALA3887775	B	PTP pNPP Assay: PTP activity was assayed using p-nitrophenyl phosphate (pNPP) as a substrate in DMG buffer (50 mM DMG, pH 7.0, 1 mM EDTA, 150 mM NaCl, 2 mM DTT, 0.1 mg/mL BSA) at 25° C. The assays were performed in 96-well plates. Normally, to determine the IC50 values, the reaction was initiated by the addition of enzyme (final concentration of 10 nM) to a reaction mixture (0.2 mL) containing 2 mM (Km for the substrate) pNPP with various concentrations of inhibitors. The reaction rate was measured using a Spectra Max Plus 384 Microplate Spectrophotometer (Molecular Devices). For compound 10, the final TC-PTP concentration was 0.04 nM. The reactions were incubated at room temperature for 30 min and quenched with 5N NaOH. The absorbance at 405 nm was read on the SpectraMax Plus 384 Microplate Spectrophotometer. For the reversibility test, the enzyme was incubated with the inhibitor at room temperature for 30 min before pNPP was added to initiate the reaction. The reaction was then incubated at room temperature.	Homo sapiens	1		single protein format	BindingDB Database
15.	ALA3888467	B	Enzyme Kinetic Assay: PTP activity was assayed using p-nitrophenyl phosphate (pNPP) as a substrate in 3,3-dimethylglutarate buffer (50 mM 3,3-dimethylglutarate, pH 7.0, 1 mM EDTA, 150 mM NaCl, 2 mM DTT, 0.1 mg/mL BSA) at 25° C. The assays were performed in 96-well plates. Normally, to determine the IC50 values, the reaction was initiated by the addition of enzyme (final concentration at 10 nM) to a reaction mixture (0.2 mL) containing 2 mM (Km for the substrate) pNPP with various concentrations of inhibitors. The reaction rate was measured using a SpectraMax Plus 384 Microplate Spectrophotometer (Molecular Devices). To determine the mode of inhibition, the reactions were initiated by the addition of PTP-MEG2 to the reaction mixtures (0.2 mL) containing various concentrations of pNPP with different concentrations of the inhibitor 7.	Homo sapiens	1		single protein format	BindingDB Database
16.	ALA4121963	B	Inhibition of allosterically sensitized mutant form of His6-tagged human FAP1 catalytic domain at 500 nM using pNPP as substrate incubated for 90 mins by quenched phosphatase activity assay	Homo sapiens	4		single protein format	Scientific Literature
17.	ALA4121966	B	Inhibition of allosterically sensitized mutant form of His6-tagged human FAP1 catalytic domain using pNPP as substrate after 90 mins by quenched phosphatase activity assay	Homo sapiens	1		single protein format	Scientific Literature
18.	ALA4121971	B	Inhibition of allosterically sensitized mutant form of His6-tagged human FAP1 at 1000 nM using pNPP as substrate by quenched phosphatase activity assay	Homo sapiens	1		single protein format	Scientific Literature
19.	ALA4417885	B	Inhibition of PTPN13 (unknown origin) assessed as change in enzyme activity at 150 uM using PNPP substrate incubated for 15 mins relative to control	Homo sapiens	1		single protein format	Patent Bioactivity Data
20.	ALA4626300	A	Inhibition of FAP1 (unknown origin) expressed in Escherichia coli BL21 using p-nitrophenyl phosphate as substrate measured after 30 mins by UV-vis spectrophotometric method	Homo sapiens	3		assay format	Scientific Literature