#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3591180	Binding	Inhibition of human recombinant CDK9 expressed in insect cells using CDK7/9 tide as substrate after 30 mins by scintillation counting analysis	Homo sapiens	16	ALA3588821	cell-based format	Scientific Literature
2.	ALA3599524	Binding	Inhibition of CDK9 (unknown origin) at 0.5 uM relative to control	Homo sapiens	3	ALA3596148	single protein format	Scientific Literature
3.	ALA3630643	Binding	Inhibition of CDK9 (unknown origin) assessed as remaining activity at 10 uM by high-throughput assay relative to control	Homo sapiens	1	ALA3627674	single protein format	Scientific Literature
4.	ALA3706262	Binding	In Vitro Kinase Inhibition Assays: All kinase assays were performed in 96-well FlashPlates from Perkin Elmer/NEN (Boston, Mass., USA) in a 50 μl reaction volume. The reaction mixture was pipetted in four steps in the following order:20 μl of assay buffer (standard buffer),5 μl of ATP solution (in H2O),5 μl of test compound (in 10% DMSO),10 μl of substrate/10 μl of enzyme solution (premixed),The assay for all enzymes contained 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-Orthovanadate, 1.2 mM DTT, 50 μg/ml PEG20000, 1 μM [-33P]-ATP (approx. 5×1005 cpm per well).	Homo sapiens	10	ALA3638952	single protein format	BindingDB Database
5.	ALA3706310	Binding	LANCE ULight TR-FRET Kinase Assay: CDK9 enzyme activities were measured using LANCE ULight TR-FRET kinase assay reagents (PerkinElmer, Waltham, Mass.). Compounds were directly added in 100% DMSO to white low volume assay plates (Perkin Elmer Proxiplate 6008289) using a Labcyte Echo acoustic dispenser. Assay reagents in serine/threonine kinase assay buffer containing 20 mM HEPES, 10 mM MgCl2, 100 mM Na3VO4, and 0.0075% Triton X-100. were added for final reaction mixture concentrations of 1000 uM ATP, 100 nM U-light MBP peptide (Perkin Elmer TRF0109M) and reaction initiated with 4 nM CDK9/Cyclin T1 (Carna Biosciences 04-110). The kinase reaction was carried out for 30 minutes before addition of stopping buffer to a final of 20 mM EDTA and 0.5 nM of LANCE Ultra Europium anti-phospho-MBP antibody (PerkinElmer TRF0201M) in LANCE detection buffer (PerkinElmer CR97-100). The reaction was equilibrated for 1 hour and the signal read in the Perkin Elmer Envision in TR-FRET mode.	Homo sapiens	368	ALA3639207	single protein format	BindingDB Database
6.	ALA3707939	Binding	In Vitro Kinase Inhibition Assay: In vitro kinase assay analysis may be performed using standard techniques described in the art. These techniques are also used by commercial services providers in order to offer in vitro kinase activity assay services, e.g. the assays offered by Millipore Inc. (worldwide website www(dot)millipore.com), ProQinase GmbH (worldwide website www(dot)proqinase.de) and others. All kinase assays were performed in 96-well FlashPlates from Perkin Elmer/NEN (Boston, Mass., USA) in a 50ul reaction volume. The reaction mixture was pipetted in four steps in the following order: 20ul of assay buffer (standard buffer),5ul of ATP solution (in H2O, 5 ul of test compound (in 10% DMSO), 10ul of substrate/10ul of enzyme solution (premixed)The assay for all enzymes contained 60 mM HEPES-NaOH (pH 7.5), 3 mM MgCl2, 3 mM MnCl2, 3uM Na-Orthovanadate, 1.2 mM DTT, 50 ug/ml PEG2000, 1 uM [33P]-ATP (approx. 5x1005 cpm per well).	Homo sapiens	2	ALA3638701	single protein format	BindingDB Database
7.	ALA3707641	Binding	Kinase Assay: To evaluate the in-vitro kinase potency of the compounds, they were screened against CDK 2 and CDK9. Kinase assays were performed in 96-well plates using recombinant CDK/cyclins generated at Cyclacel. Ltd Dundee, UK.	Homo sapiens	16	ALA3638727	single protein format	BindingDB Database
8.	ALA3771439	Binding	Inhibition of human recombinant CDK9 assessed as residual activity at 10 uM after 1 hr by qPCR analysis relative to control	Homo sapiens	1	ALA3769377	single protein format	Scientific Literature
9.	ALA3777660	Binding	Inhibition of CDK9 (unknown origin)	Homo sapiens	4	ALA3774312	single protein format	Scientific Literature
10.	ALA3782158	Binding	Inhibition of partial length human CDK9 expressed in bacterial system at 1 uM	Homo sapiens	1	ALA3779767	assay format	Scientific Literature
11.	ALA3803172	Binding	Inhibition of CDK9 (unknown origin)	Homo sapiens	1	ALA3797138	single protein format	Scientific Literature
12.	ALA3812235	Binding	Inhibition of full length human CDK9 expressed in bacterial system assessed as enzyme activity at 10 uM by KINOMEScan assay	Homo sapiens	1	ALA3808372	assay format	Scientific Literature
13.	ALA3825313	Binding	Inhibition of CDK9 (unknown origin)	Homo sapiens	2	ALA3822394	single protein format	Scientific Literature
14.	ALA870945	Binding	Inhibition of CDK9	Homo sapiens	4	ALA1137419	single protein format	Scientific Literature
15.	ALA942743	Binding	Inhibition of CDK9		1	ALA1140564	single protein format	Scientific Literature
16.	ALA1069289	Binding	Inhibition of CDK9 at 10 uM		2	ALA1152632	single protein format	Scientific Literature
17.	ALA1103003	Binding	Inhibition of CDK9		1	ALA1154951	single protein format	Scientific Literature
18.	ALA1114045	Binding	Inhibition of CDK9 at 5 uM		1	ALA1154316	single protein format	Scientific Literature
19.	ALA1111591	Binding	Inhibition of CDK9 at 10 uM		1	ALA1156291	single protein format	Scientific Literature
20.	ALA1119484	Binding	Inhibition of CDK9		16	ALA1155519	single protein format	Scientific Literature