# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3734544 | Binding | Inhibition of recombinant CK-alpha (unknown origin) expressed in Escherichia coli BL21 (DE3) cells | Homo sapiens | 5 | ALA3727353 | cell-based format | Patent Bioactivity Data | |
2. | ALA3769127 | Binding | Binding affinity to human N-terminal truncated ChoKalpha1 (75 to 457 residues) by surface plasmon resonance assay | Homo sapiens | 20 | ALA3763032 | single protein format | Scientific Literature | |
3. | ALA3769131 | Binding | Binding affinity to human N-terminal truncated ChoKalpha1 (75 to 457 residues) assessed as ligand residence time by surface plasmon resonance assay | Homo sapiens | 7 | ALA3763032 | single protein format | Scientific Literature | |
4. | ALA3769132 | Binding | Binding affinity to human N-terminal truncated ChoKalpha1 (75 to 457 residues) assessed as association rate constant by surface plasmon resonance assay | Homo sapiens | 7 | ALA3763032 | single protein format | Scientific Literature | |
5. | ALA3769133 | Binding | Inhibition of human N-terminal truncated ChoKalpha1 (75 to 457 residues) using choline chloride as substrate measured over 10 to 30 mins by coupled ATP regeneration assay | Homo sapiens | 20 | ALA3763032 | single protein format | Scientific Literature | |
6. | ALA3766469 | Binding | Inhibition of ChoKalpha in human MDA-MB-468 cells assessed as reduction in phosphocholine level after 24 hrs by NMR analysis | Homo sapiens | 1 | ALA3763032 | cell-based format | Scientific Literature | |
7. | ALA3766470 | Binding | Inhibition of ChoKalpha in human MDA-MB-415 cells assessed as reduction in phosphocholine level after 24 hrs by NMR analysis | Homo sapiens | 1 | ALA3763032 | cell-based format | Scientific Literature | |
8. | ALA3766475 | Binding | Inhibition of recombinant human ChoKalpha using choline as substrate by ultraviolet spectroscopic assay | Homo sapiens | 1 | ALA3763032 | single protein format | Scientific Literature | |
9. | ALA3766476 | Binding | Reversible binding affinity to human N-terminal truncated ChoKalpha1 (75 to 457 residues) ChoKalpha1 by STD NMR analysis | Homo sapiens | 1 | ALA3763032 | single protein format | Scientific Literature | |
10. | ALA662980 | Binding | Ex vivo inhibitory activity against human choline kinase was determined | 5 | ALA1145059 | single protein format | Scientific Literature | ||
11. | ALA662981 | Binding | Compound was tested ex vivo for inhibition against purified choline kinase obtained from yeast | Saccharomyces cerevisiae | 10 | ALA1133498 | single protein format | Scientific Literature | |
12. | ALA828518 | Binding | Inhibitory activity against human choline kinase enzyme | Homo sapiens | 25 | ALA1138794 | single protein format | Scientific Literature | |
13. | ALA832433 | Binding | Ex vivo inhibitory concentration against human choline kinase | Homo sapiens | 41 | ALA1144422 | single protein format | Scientific Literature | |
14. | ALA1772322 | Binding | Inhibition of ChoK in HT29 cells using [methyl-14C]choline chloride as substrate after 14 hrs by radiography | 2 | ALA1770085 | cell-based format | Scientific Literature | ||
15. | ALA1912789 | Binding | Inhibition of recombinant human Choline kinase alpha using [methyl-14C]choline chloride as substrate after 30 mins by autoradiography | Homo sapiens | 17 | ALA1909613 | single protein format | Scientific Literature | |
16. | ALA3117184 | Binding | Binding affinity to human CK-alpha1 by tryptophan fluorescence spectroscopic analysis | Homo sapiens | 3 | ALA3112426 | single protein format | Scientific Literature | |
17. | ALA3227786 | Binding | Inhibition of CHKA in human HCT116 cells assessed as [3H]phosphocholine formation using [3H]choline chloride as substrate at 2.4 +/-1 uM incubated for 1 hr prior to substrate addition measured after 1 hr by scintillation counting analysis | Homo sapiens | 2 | ALA3217582 | cell-based format | Scientific Literature | |
18. | ALA3227787 | Binding | Inhibition of CHKA in human HCT116 cells assessed as [3H]phosphocholine formation using [3H]choline chloride as substrate at 6.5 +/-4 uM incubated for 1 hr prior to substrate addition measured after 1 hr by scintillation counting analysis | Homo sapiens | 2 | ALA3217582 | cell-based format | Scientific Literature | |
19. | ALA3227789 | Binding | Inhibition of CHKA in human HCT116 cells assessed as [3H]phosphocholine formation using [3H]choline chloride as substrate incubated for 1 hr prior to substrate addition measured after 1 hr by scintillation counting analysis | Homo sapiens | 2 | ALA3217582 | cell-based format | Scientific Literature | |
20. | ALA4015086 | Binding | Inhibition of human CHKalpha (1 to 457 residues) by ADP Glo HTS assay | Homo sapiens | 1 | ALA4014298 | single protein format | Scientific Literature |