| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3762925 | B | Inhibition of human notum pectinacetylesterase expressed in 293F cells by TCF/LEF CellSensor assay | Homo sapiens | 42 | cell-based format | Scientific Literature | ||
| 2. | ALA3778288 | B | Inhibition of human notum pectinacetylesterase expressed in 293F cells after 30 mins by TCF/LEF cell sensor assay | Homo sapiens | 28 | cell-based format | Scientific Literature | ||
| 3. | ALA4150561 | B | Inhibition of NOTUM in human SW620 cells conditioned medium assessed as preservation of Wnt3A activity pretreated for 1 hr followed by Wnt3A producing L-cell addition for 2 hrs and subsequent HEK393-STF cell addition and measured after 24 hrs by luciferase reporter gene assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 4. | ALA4150562 | B | Inhibition of rhodamine-tagged FP probe binding to recombinant human C-terminal FLAG-tagged NOTUM expressed in HEK293T cells pretreated for 30 mins followed by rhodamine-tagged FP probe addition and measured after 30 mins by SDS-PAGE fluorescence analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 5. | ALA4150563 | B | Inhibition of rhodamine-tagged FP probe binding to NOTUM in human SW620 cells conditioned media pretreated for 30 mins followed by rhodamine-tagged FP probe addition and measured after 30 mins by SDS-PAGE fluorescence analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 6. | ALA4150564 | B | Inhibition of rhodamine-tagged FP probe binding to NOTUM in human SW620 cells conditioned media at 0.1 to 3 uM pretreated for 30 mins followed by rhodamine-tagged FP probe addition and measured after 30 mins by SDS-PAGE fluorescence analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 7. | ALA4150568 | B | Inhibition of NOTUM in human SW620 cells conditioned media at 0.5 uM after 1 hr by FP-biotin probe based LC-MS analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4150569 | B | Inhibition of NOTUM in human SW620 cells conditioned medium at 10 uM after 1 hr by FP-biotin probe based LC-MS analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4150570 | B | Inhibition of NOTUM in human SW620 cells at 0.5 to 10 uM after 2 hrs by FP-biotin probe based LC-MS analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4150572 | B | Inhibition of rhodamine-tagged FP probe binding to NOTUM in human SW620 cells conditioned medium at 0.01 to 1 uM pretreated for 30 mins followed by rhodamine-tagged FP probe addition and measured after 30 mins by SDS-PAGE fluorescence analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4150573 | B | Inhibition of NOTUM in human SW620 cells conditioned medium at 0.01 to 1 uM measured after 30 mins by rhodamine-azide reporter tagged based SDS-PAGE fluorescence analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4334738 | B | Inhibition of His6-tagged NOTUM purified from human HEK293S cells using OPTS as substrate incubated for 1 hr by fluorescence based assay | Homo sapiens | 49 | assay format | Scientific Literature | ||
| 13. | ALA4334739 | B | Inhibition of His6-tagged NOTUM purified from human HEK293S cells at 100 uM using OPTS as substrate incubated for 1 hr by fluorescence based assay relative to control | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 14. | ALA4334742 | B | Inhibition of NOTUM in human HEK293S cells harbouring TCF/LEF luciferase reporter gene assessed as activation wnt signalling pathway at 10 uM incubated for 10 mins followed by wnt-3A addition further incubated for 1 hr by steady-glo luciferase assay relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 15. | ALA4334743 | B | Inhibition of NOTUM in human HEK293S cells harbouring TCF/LEF luciferase reporter gene assessed as activation wnt signalling pathway at 3 uM incubated for 10 mins followed by wnt-3A addition further incubated for 1 hr by steady-glo luciferase assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4334744 | B | Inhibition of NOTUM in human HEK293S cells harbouring TCF/LEF luciferase reporter gene assessed as activation wnt signalling pathway incubated for 10 mins followed by wnt-3A addition further incubated for 1 hr by steady-glo luciferase assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 17. | ALA4334745 | B | Competitive inhibition of NOTUM in human HEK293S cells at 10 uM incubated for 30 mins followed by FP-biotin addition and measured after 10 mins by immunoblot analysis | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 18. | ALA4610952 | B | Inhibition of human His6-tagged NOTUM (81 to 451 residues) Cys330Ser mutant transfected in HEK293S cells using OPTS as substrate incubated for 1 hr by fluorescence based assay | Homo sapiens | 50 | cell-based format | Scientific Literature | ||
| 19. | ALA4610953 | B | Inhibition of NOTUM (unknown origin) transfected in human HEK293T cells harbouring TCF/LEF luciferase reporter gene assessed as activation of Wnt signalling pathway incubated for 10 mins followed by wnt-3A addition and further incubated for 1 hr by steady-glo luciferase assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 20. | ALA4610954 | B | Competitive inhibition of human His6-tagged NOTUM (81 to 451 residues) Cys330Ser mutant transfected in HEK293S assessed as inhibition of NOTUM biotinylation at 3 uM incubated for 30 mins followed by FP-biotin addition and measured after 10 mins by immunoblot analysis | Homo sapiens | 3 | assay format | Scientific Literature |
The page will load shortly, Thanks for your patience!