#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3762925	Binding	Inhibition of human notum pectinacetylesterase expressed in 293F cells by TCF/LEF CellSensor assay	Homo sapiens	42	ALA3758167	cell-based format	Scientific Literature
2.	ALA3778288	Binding	Inhibition of human notum pectinacetylesterase expressed in 293F cells after 30 mins by TCF/LEF cell sensor assay	Homo sapiens	28	ALA3774323	cell-based format	Scientific Literature
3.	ALA4150561	Binding	Inhibition of NOTUM in human SW620 cells conditioned medium assessed as preservation of Wnt3A activity pretreated for 1 hr followed by Wnt3A producing L-cell addition for 2 hrs and subsequent HEK393-STF cell addition and measured after 24 hrs by luciferase reporter gene assay	Homo sapiens	2	ALA4145632	cell-based format	Scientific Literature
4.	ALA4150562	Binding	Inhibition of rhodamine-tagged FP probe binding to recombinant human C-terminal FLAG-tagged NOTUM expressed in HEK293T cells pretreated for 30 mins followed by rhodamine-tagged FP probe addition and measured after 30 mins by SDS-PAGE fluorescence analysis	Homo sapiens	2	ALA4145632	cell-based format	Scientific Literature
5.	ALA4150563	Binding	Inhibition of rhodamine-tagged FP probe binding to NOTUM in human SW620 cells conditioned media pretreated for 30 mins followed by rhodamine-tagged FP probe addition and measured after 30 mins by SDS-PAGE fluorescence analysis	Homo sapiens	6	ALA4145632	cell-based format	Scientific Literature
6.	ALA4150564	Binding	Inhibition of rhodamine-tagged FP probe binding to NOTUM in human SW620 cells conditioned media at 0.1 to 3 uM pretreated for 30 mins followed by rhodamine-tagged FP probe addition and measured after 30 mins by SDS-PAGE fluorescence analysis	Homo sapiens	3	ALA4145632	cell-based format	Scientific Literature
7.	ALA4150568	Binding	Inhibition of NOTUM in human SW620 cells conditioned media at 0.5 uM after 1 hr by FP-biotin probe based LC-MS analysis relative to control	Homo sapiens	1	ALA4145632	cell-based format	Scientific Literature
8.	ALA4150569	Binding	Inhibition of NOTUM in human SW620 cells conditioned medium at 10 uM after 1 hr by FP-biotin probe based LC-MS analysis relative to control	Homo sapiens	1	ALA4145632	cell-based format	Scientific Literature
9.	ALA4150570	Binding	Inhibition of NOTUM in human SW620 cells at 0.5 to 10 uM after 2 hrs by FP-biotin probe based LC-MS analysis	Homo sapiens	1	ALA4145632	cell-based format	Scientific Literature
10.	ALA4150572	Binding	Inhibition of rhodamine-tagged FP probe binding to NOTUM in human SW620 cells conditioned medium at 0.01 to 1 uM pretreated for 30 mins followed by rhodamine-tagged FP probe addition and measured after 30 mins by SDS-PAGE fluorescence analysis	Homo sapiens	1	ALA4145632	cell-based format	Scientific Literature
11.	ALA4150573	Binding	Inhibition of NOTUM in human SW620 cells conditioned medium at 0.01 to 1 uM measured after 30 mins by rhodamine-azide reporter tagged based SDS-PAGE fluorescence analysis	Homo sapiens	1	ALA4145632	cell-based format	Scientific Literature
12.	ALA4334738	Binding	Inhibition of His6-tagged NOTUM purified from human HEK293S cells using OPTS as substrate incubated for 1 hr by fluorescence based assay	Homo sapiens	49	ALA4334426	assay format	Scientific Literature
13.	ALA4334739	Binding	Inhibition of His6-tagged NOTUM purified from human HEK293S cells at 100 uM using OPTS as substrate incubated for 1 hr by fluorescence based assay relative to control	Homo sapiens	2	ALA4334426	assay format	Scientific Literature
14.	ALA4334742	Binding	Inhibition of NOTUM in human HEK293S cells harbouring TCF/LEF luciferase reporter gene assessed as activation wnt signalling pathway at 10 uM incubated for 10 mins followed by wnt-3A addition further incubated for 1 hr by steady-glo luciferase assay relative to control	Homo sapiens	2	ALA4334426	cell-based format	Scientific Literature
15.	ALA4334743	Binding	Inhibition of NOTUM in human HEK293S cells harbouring TCF/LEF luciferase reporter gene assessed as activation wnt signalling pathway at 3 uM incubated for 10 mins followed by wnt-3A addition further incubated for 1 hr by steady-glo luciferase assay relative to control	Homo sapiens	1	ALA4334426	cell-based format	Scientific Literature
16.	ALA4334744	Binding	Inhibition of NOTUM in human HEK293S cells harbouring TCF/LEF luciferase reporter gene assessed as activation wnt signalling pathway incubated for 10 mins followed by wnt-3A addition further incubated for 1 hr by steady-glo luciferase assay	Homo sapiens	3	ALA4334426	cell-based format	Scientific Literature
17.	ALA4334745	Binding	Competitive inhibition of NOTUM in human HEK293S cells at 10 uM incubated for 30 mins followed by FP-biotin addition and measured after 10 mins by immunoblot analysis	Homo sapiens	5	ALA4334426	cell-based format	Scientific Literature
18.	ALA4610952	Binding	Inhibition of human His6-tagged NOTUM (81 to 451 residues) Cys330Ser mutant transfected in HEK293S cells using OPTS as substrate incubated for 1 hr by fluorescence based assay	Homo sapiens	50	ALA4610015	cell-based format	Scientific Literature
19.	ALA4610953	Binding	Inhibition of NOTUM (unknown origin) transfected in human HEK293T cells harbouring TCF/LEF luciferase reporter gene assessed as activation of Wnt signalling pathway incubated for 10 mins followed by wnt-3A addition and further incubated for 1 hr by steady-glo luciferase assay	Homo sapiens	5	ALA4610015	cell-based format	Scientific Literature
20.	ALA4610954	Binding	Competitive inhibition of human His6-tagged NOTUM (81 to 451 residues) Cys330Ser mutant transfected in HEK293S assessed as inhibition of NOTUM biotinylation at 3 uM incubated for 30 mins followed by FP-biotin addition and measured after 10 mins by immunoblot analysis	Homo sapiens	3	ALA4610015	assay format	Scientific Literature