#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3734144	Binding	Inhibition of PFKFB4 (unknown origin) expressed in Escherichia coli assessed as ADP level preincubated for 15 mins followed by addition of fructose-6-phosphate as substrate and ATP measured after 40 mins by luciferase assay	Homo sapiens	18	ALA3727345	assay format	Patent Bioactivity Data
2.	ALA3887842	Binding	Kinase Assay (Method B): The kinase activity of the bi-functional enzyme is readily quantified based on the production of ADP and F-2,6-P2 from ATP and F6P. The ADP is detected with a kit, ADP-Glo Kinase Assay(a-e), from Promega. The assay is a luminescent ADP detection assay that measures kinase activity by quantifying the amount of ADP produced during the kinase reaction. The assay is performed in two steps; first, after the kinase reaction, an equal volume of ADP-Glo Reagent is added to terminate the kinase reaction and deplete the remaining ATP. Second, the Kinase Detection Reagent is added to simultaneously convert ADP to ATP and allow the newly synthesized ATP to be measured using a luciferase/luciferine reaction. The light generated is measured using a luminescence counter (1450 MicroBeta TriLux).The assay was performed in white 384-well microtiter plates (OptiPlate, 6007299, PerkinElmer) by consecutive additions of 0.1 uL of a test compound solution.		18	ALA3886470	single protein format	BindingDB Database
3.	ALA5230610	Binding	Inhibition of PFKFB4 (unknown origin) at 5.94 uM relative to control	Homo sapiens	1	ALA5230092	single protein format	Scientific Literature