| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3772452 | B | Reversible inhibition of His-tagged recombinant human dCTPase using dCTP as substrate assessed as remaining activity at 10 times IC50 preincubated for 1 hr followed by 100 fold dilution with substrate measured after 10 to 15 mins by jump dilution assay | Homo sapiens | 2 | ALA3769365 | single protein format | Scientific Literature | |
| 2. | ALA3772458 | B | Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate at 10 uM after 1 hr by HTS-adapted Malachite Green assay | Homo sapiens | 1 | ALA3769365 | assay format | Scientific Literature | |
| 3. | ALA3772975 | B | Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay | Homo sapiens | 34 | ALA3769365 | assay format | Scientific Literature | |
| 4. | ALA3772976 | B | Inhibition of human recombinant DCTPP1 expressed in Escherichia coli BL21 Star (DE3) using dCTP as substrate by PPiLight assay | Homo sapiens | 1 | ALA3769365 | assay format | Scientific Literature | |
| 5. | ALA3772977 | B | Stabilization of recombinant human dCTPase expressed in Escherichia coli BL21(DE3)pLysS at 0.001 to 100 uM by differential scanning fluorimetry assay | Homo sapiens | 2 | ALA3769365 | assay format | Scientific Literature | |
| 6. | ALA3772978 | B | Stabilization of dCTPase in human HL60 cells at 0.6 to 10 uM preincubated for 2 hrs followed by heating at 67 degC by cellular thermal shift assay | Homo sapiens | 1 | ALA3769365 | cell-based format | Scientific Literature | |
| 7. | ALA3991684 | B | Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry. | Homo sapiens | 243 | ALA3991601 | subcellular format | Kuster lab chemical proteomics drug profiling | |
| 8. | ALA4008373 | B | Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay | Homo sapiens | 58 | ALA4007403 | assay format | Scientific Literature | |
| 9. | ALA4008375 | B | Binding affinity to recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS assessed as change in melting temperature at 33 uM by Sypro Orange dye based DSF assay | Homo sapiens | 5 | ALA4007403 | assay format | Scientific Literature | |
| 10. | ALA4008378 | B | Stabilization of dCTPase 1 in human MCF7 cells at 5 to 20 uM at 65 to 75 degC after 2 hrs by Western blot analysis based whole cell thermal shift assay | Homo sapiens | 1 | ALA4007403 | cell-based format | Scientific Literature | |
| 11. | ALA4008401 | B | Inhibition of dCTPase 1 in human HL60 cells assessed as potentiation of 5-AzaC-induced cytotoxicity by measuring decrease in cell viability at 1 to 5 uM after 72 hrs by resazurin assay | Homo sapiens | 3 | ALA4007403 | cell-based format | Scientific Literature | |
| 12. | ALA4008403 | B | Inhibition of dCTPase 1 in human HL60 cells assessed as potentiation of 0.5 to 10 uM 5-AzaC-induced cytotoxicity by measuring decrease in cell viability after 72 hrs by resazurin assay | Homo sapiens | 1 | ALA4007403 | cell-based format | Scientific Literature | |
| 13. | ALA4008404 | B | Inhibition of dCTPase 1 (unknown origin) expressed in Escherichia coli BL21 Star (DE3) using dCTP as substrate by luminescence assay | Homo sapiens | 1 | ALA4007403 | assay format | Scientific Literature | |
| 14. | ALA4016017 | B | Inhibition of recombinant human dCTPase assessed as increase in melting temperature at 33 uM by SYPRO orange dye based differential scanning fluorimetric analysis | Homo sapiens | 5 | ALA4014328 | single protein format | Scientific Literature | |
| 15. | ALA4016018 | B | Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) at 10 uM using dCTP as substrate by HTS-based malachite green assay | Homo sapiens | 1 | ALA4014328 | assay format | Scientific Literature | |
| 16. | ALA4016019 | B | Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay | Homo sapiens | 27 | ALA4014328 | assay format | Scientific Literature | |
| 17. | ALA4016020 | B | Inhibition of dCTPase in human MCF7 cells assessed as thermal stabilization up to 20 uM after 2 hrs at 65 to 75 degC by Western blot method | Homo sapiens | 1 | ALA4014328 | cell-based format | Scientific Literature | |
| 18. | ALA4016021 | B | Inhibition of dCTPase in human HL60 cells assessed as protection against pronase digestion at 10 uM after preincubated for 4 hrs followed by pronase addition for 30 mins by DARTS assay | Homo sapiens | 1 | ALA4014328 | cell-based format | Scientific Literature | |
| 19. | ALA4044324 | B | Inhibition of full-length recombinant human dCTPase at 10 uM by microtiter plate-adapted malachite green assay | Homo sapiens | 38 | ALA4043098 | single protein format | Scientific Literature | |
| 20. | ALA4044325 | B | Inhibition of full-length recombinant human dCTPase at by microtiter plate-adapted malachite green assay | Homo sapiens | 30 | ALA4043098 | single protein format | Scientific Literature |
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