| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3776336 | B | Inhibition of KDM5B (unknown origin) expressed in Escherichia coli using H3 (1 to 21 residues) K4Me3-GGK-Biotin KDM5B Cognate Ligand/10 uM alpha-ketoglutarate as substrate/cofactor preincubated for 5 mins followed by substrate addition measured after 20 mins by alphascreen assay | Homo sapiens | 6 | assay format | Scientific Literature | ||
| 2. | ALA3778807 | B | Inhibition of KDM5B (unknown origin) using biotin-H3K4me3 as substrate preincubated for 15 mins followed by substrate addition measured after 20 mins by alphascreen assay | Homo sapiens | 56 | single protein format | Scientific Literature | ||
| 3. | ALA3776211 | B | Inhibition of KDM5B (unknown origin) using biotin-H3K4me3 as substrate at 100 uM preincubated for 15 mins followed by substrate addition measured after 20 mins by alphascreen assay relative to control | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 4. | ALA3776401 | B | Inhibition of full length N-terminal FLAG-tagged KDM5B (unknown origin) expressed in human HeLa cells assessed as increase in H3K4me3 level after 24 hrs by DAPI staining based immunofluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA3776404 | B | Inhibition of full length N-terminal FLAG-tagged KDM5B (unknown origin) expressed in human HeLa cells assessed as global hypomethylation after 24 hrs by DAPI staining based immunofluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA3782535 | B | Inhibition of human KDM5B preincubated for 10 mins followed by substrate addition by AlphaLISA assay | Homo sapiens | 6 | single protein format | Scientific Literature | ||
| 7. | ALA3788933 | B | Inhibition of recombinant human N-terminal His-FLAG-tagged KDM5B (2 to 751 residues) expressed in baculovirus infected sf9 cells using biotin-H3K4me3 substrate incubated for 30 mins by TR-FRET assay | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
| 8. | ALA3788938 | B | Inhibition of KDM5B in human ZR-75-1 cells assessed as reduction in H3K4me3 demethylation incubated for 72 hrs by fluorometric immunoassay | Homo sapiens | 9 | cell-based format | Scientific Literature | ||
| 9. | ALA3789198 | B | Inhibition of KDM5B (unknown origin) | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 10. | ALA3789202 | B | Inhibition of recombinant full length FLAG-tagged KDM5B (unknown origin) expressed in sf9 cells using biotin-H3K4me3 (1 to 21 residues as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by mass spetcrometric analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 11. | ALA3789204 | B | Inhibition of KDM5B in human PC9 cells assessed as reduction in demethylation of H3K4me3 after 120 hrs by AlphaLisa assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 12. | ALA3789212 | B | Inhibition of KDM5B (1 to 809 residues) (unknown origin) expressed in Escherichia coli using biotin-H3K4me3 as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by AlphaLisa assay | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 13. | ALA3789447 | B | Inhibition of KDM5B in human U2OS cells assessed as reduction in demethylation of H3K4me3 after 20 hrs by Hoechst 33342 staining based immunofluorescence assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 14. | ALA3795797 | B | Inhibition of KDM5B (unknown origin) using substrate peptide/ascorbate/2-OG/ Fe(2) as substrate preincubated for 15 mins followed by addition of substrate peptide/ ascorbate/2-OG/ Fe(2) measured after 1 hr by AlphaLISA method | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA4035859 | B | Inhibition of recombinant human N-terminal FLAG-tagged KDM5B expressed in baculovirus infected Sf9 insect cells using H3K4me3 peptide as substrate after 30 to 45 mins by TR-FRET assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4193856 | B | Inhibition of KDM5B (unknown origin) using biotinylated H3K4me3 peptide and alpha-ketoglutarate as substrate pretreated for 1 hr followed by substrate addition measured after 20 mins by LANCE TR-FRET assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA4223291 | B | Inhibition of KDM5B (unknown origin) preincubated with enzyme | Homo sapiens | 34 | single protein format | Scientific Literature | ||
| 18. | ALA4256950 | B | Inhibition of recombinant human N-terminal His-FLAG tagged JARID1B (2 to 751 residues) expressed in baculovirus infected sf9 cells using H3K4me3-biotin peptide as substrate after 30 mins in presence of alpha-ketoglutaric acid by LANCE TR-FRET assay | Homo sapiens | 144 | cell-based format | Patent Bioactivity Data | ||
| 19. | ALA4257002 | B | Inhibition of recombinant human N-terminal His-FLAG-tagged JARID1B (2 to 751 residues) expressed in baculovirus infected Sf9 cells using H3K4me3-biotin labelled peptide as substrate measured after 30 mins by Lance TR-FRET assay | Homo sapiens | 51 | cell-based format | Patent Bioactivity Data | ||
| 20. | ALA4257281 | B | Inhibition of recombinant human N-terminal His-FLAG tagged JARID1B (2 to 751 residues) expressed in baculovirus infected sf9 cells using H3K4me3-biotin peptide as substrate after 30 mins in presence of alpha-ketoglutaric acid by LANCE TR-FRET assay | Homo sapiens | 122 | cell-based format | Patent Bioactivity Data |
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