# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3782532 | B | Inhibition of human KDM2B preincubated for 10 mins followed by substrate addition by AlphaLISA assay | Homo sapiens | 6 | ALA3779836 | single protein format | Scientific Literature | |
2. | ALA3788936 | B | Inhibition of recombinant human C-terminal FLAG-tagged KDM2B (1 to 650 residues) expressed in baculovirus infected sf9 cells using biotin-H3K36me2 substrate incubated for 30 mins by alphascreen assay | Homo sapiens | 5 | ALA3784917 | cell-based format | Scientific Literature | |
3. | ALA3789206 | B | Inhibition of recombinant human C-terminal FLAG-tagged KDM2B (1 to 650 residues) expressed in baculovirus infected sf9 cells using biotin-H3K36me2 as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by AlphaLisa assay | Homo sapiens | 5 | ALA3784917 | cell-based format | Scientific Literature | |
4. | ALA4022351 | B | Inhibition of KDM2B (unknown origin) at 10 uM | Homo sapiens | 2 | ALA4020786 | single protein format | Scientific Literature | |
5. | ALA4022355 | B | Inhibition of KDM2B (unknown origin) | Homo sapiens | 1 | ALA4020786 | single protein format | Scientific Literature | |
6. | ALA4035838 | B | Inhibition of recombinant human N-terminal FLAG-tagged KDM2B expressed in baculovirus infected Sf9 insect cells using H3K36me2 peptide as substrate after 30 to 45 mins by TR-FRET assay | Homo sapiens | 2 | ALA4033700 | cell-based format | Scientific Literature | |
7. | ALA4193850 | B | Inhibition of KDM2B (unknown origin) using biotinylated H3K36me2 peptide and alpha-ketoglutarate as substrate pretreated for 1 hr followed by substrate addition measured after 20 mins by LANCE TR-FRET assay | Homo sapiens | 1 | ALA4190335 | single protein format | Scientific Literature | |
8. | ALA4223297 | B | Inhibition of KDM2B (unknown origin) | Homo sapiens | 1 | ALA4219204 | single protein format | Scientific Literature | |
9. | ALA4256953 | B | Inhibition of recombinant human C-terminal FLAG-tagged FBXL10 (1 to 650 residues) expressed in Escherichia coli using H3K36me2-biotin peptide as substrate after 30 mins in presence of alpha-ketoglutaric acid by AlphaScreen assay | Homo sapiens | 52 | ALA4256941 | assay format | Patent Bioactivity Data | |
10. | ALA4256998 | B | Inhibition of recombinant human C-terminal FLAG-tagged FBXL10 (1 to 650 residues) expressed in baculovirus infected Sf9 cells using H3K36me2-biotin labelled peptide as substrate measured after 1 hr by AlphaScreen assay | Homo sapiens | 106 | ALA4256948 | cell-based format | Patent Bioactivity Data | |
11. | ALA4257001 | B | Inhibition of KDM2B in human MIAPaCa2 cells assessed as increase in protein/EZH2 repressed mRNA gene expression after 3 to 48 hrs by RT-PCR analysis | Homo sapiens | 2 | ALA4256948 | cell-based format | Patent Bioactivity Data | |
12. | ALA4257285 | B | Inhibition of recombinant human C-terminal FLAG-tagged FBXL10 (1 to 650 residues) expressed in baculovirus infected sf9 cells using H3K36me2-biotin peptide as substrate after 30 mins in presence of alpha-ketoglutaric acid by AlphaScreen assay | Homo sapiens | 150 | ALA4257223 | cell-based format | Patent Bioactivity Data | |
13. | ALA4427165 | B | Inhibition of KDM2B (unknown origin) | Homo sapiens | 1 | ALA4425149 | single protein format | Scientific Literature | |
14. | ALA4427935 | B | Inhibition of KDM2B (unknown origin) using RKSAPATGGV(KMe2)KPHRYRPGTV-NovaTagPEG-biotin/2-OG as substrate/co-factor by TR-FRET assay | Homo sapiens | 1 | ALA4425189 | single protein format | Scientific Literature | |
15. | ALA4432257 | B | Inhibition of full length human KDM2B using biotinylated H2N-RKSAPATGGV(KMe2)KPHRYRPGTV-NTPEGBiot as substrate after 2 hrs using 0.3 uM of 2-OG as co-substrate by TR-FRET assay | Homo sapiens | 2 | ALA4431331 | single protein format | Scientific Literature | |
16. | ALA5126412 | B | Inhibition of GST-tagged human KDM2B (604 to 660 residues) expressed in Escherichia coli BL21 cells incubated for 30 mins by fluorescence polarization assay | Homo sapiens | 9 | ALA5120972 | cell-based format | Scientific Literature | |
17. | ALA5127960 | B | Inhibition of KDM2B (unknown origin) | Homo sapiens | 1 | ALA5126529 | single protein format | Scientific Literature |