Reversible inhibition of recombinant Mycobacterium tuberculosis H37Rv DprE1 using FPR as substrate assessed as residual enzymatic activity preincubated for 10 mins followed by substrate addition by fluorescence assay
Irreversible inhibition of recombinant Mycobacterium tuberculosis H37Rv DprE1 using FPR as substrate assessed as residual enzymatic activity preincubated for 10 mins followed by substrate addition by fluorescence assay
Inhibition of recombinant Mycobacterium tuberculosis H37Rv DprE1 using FPR as substrate assessed as residual enzymatic activity preincubated for 10 mins followed by substrate addition by fluorescence assay
Inhibition of Mycobacterium tuberculosis H37Rv DprE1 using FPR as substrate preincubated for 6.5 hrs followed by substrate addition by resazurin dye-based spectrofluorimetric analysis
Ratio of IC50 for inhibition of Mycobacterium tuberculosis H37Rv DprE1 using FPR as substrate in presence of SAM by resazurin dye-based spectrofluorimetric analysis to IC50 for inhibition of Mycobacterium tuberculosis H37Rv DprE1 using FPR as substrate in absence of SAM by resazurin dye-based spectrofluorimetric analysis
Inhibition of N-terminal His6-thioredoxin tagged Mycobacterium tuberculosis H37Rv DprE1 expressed in Escherichia coli BL21(DE3) using FPR as substrate by resazurin dye-based fluorescence assay
Inhibition of DprE1 in Mycobacterium tuberculosis H37Rv assessed as accumulation of trehalose dimycolates at 10 times MIC measured after 24 hrs in presence of [14C]-acetate by TLC-based autoradiography
Inhibition of DprE1 in Mycobacterium tuberculosis H37Rv assessed as accumulation of trehalose monomycolates at 10 times MIC measured after 24 hrs in presence of [14C]-acetate by TLC-based autoradiography