| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3880354 | B | Inhibition of recombinant human NAEalpha/N-terminal His-tagged NAEbeta expressed in baculovirus infected insect Sf9 cells using N-terminal FLAG tagged Nedd8 as substrate incubated for 105 mins in presence of N-terminal GST-tagged Ubc12 by FRET assay | Homo sapiens | 137 | cell-based format | Patent Bioactivity Data | ||
| 2. | ALA4143718 | B | Inhibition of NAE-mediated Ubcl2-NEDD8 conjugation in human Caco2 cells at 10 uM after 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA4143719 | B | Inhibition of NAE-mediated Ubcl2-NEDD8 conjugation in human Caco2 cells after 16 hrs by Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 4. | ALA4143720 | B | Inhibition of NAE (unknown origin) assessed as decrease in NAE-mediated Ubc12-NEDD8 conjugation levels preincubated for 10 mins followed by ATP and Mg2+ addition measured after 60 mins by Western blot analysis | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 5. | ALA4143723 | B | Competitive inhibition of NAE (unknown origin) assessed as decrease in NAE-mediated Ubc12-NEDD8 conjugation levels at 10 uM preincubated for 10 mins followed by Mg2+ and various concentrations of ATP addition measured after 60 mins by Lineweaver-Burk plot analysis | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
| 6. | ALA4143724 | B | Inhibition of NAE (unknown origin) assessed as decrease in NAE-mediated Ubc12-NEDD8 conjugation levels at 10 uM treated for 10 mins post ATP and Mg2+ addition measured after 60 mins by Western blot analysis | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 7. | ALA4143725 | B | Inhibition of NAE in human Caco2 cells assessed as increase in p53 protein levels at 0.1 to 10 uM after 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4143727 | B | Inhibition of NAE in human Caco2 cells assessed as increase in p53 protein levels at 1 to 10 uM after 16 hrs by Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 9. | ALA4143728 | B | Inhibition of NAE in human Caco2 cells assessed as increase in p53 protein levels by measuring p53 degradation at 10 uM preincubated for 16 hrs followed by cycloheximide addition measured up to 60 mins by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4143729 | B | Inhibition of NAE in human Caco2 cells harboring p53-luc plasmid assessed as increase in p53 transcriptional activity after 16 hrs by luciferase reporter gene assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4143730 | B | Inhibition of NAE in human Caco2 cells assessed as increase in beta-catenin protein levels after 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4143731 | B | Inhibition of NAE in human Caco2 cells assessed as increase in c-myc protein levels after 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4143732 | B | Inhibition of NAE in human Caco2 cells assessed as increase in p27 protein levels at 0.1 to 10 uM after 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4143733 | B | Inhibition of NAE in human Caco2 cells assessed as decrease in cyclin D1 protein levels at 0.1 to 10 uM after 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4143734 | B | Inhibition of NAE in human Caco2 cells assessed as decrease in Bcl2 protein levels at 0.1 to 10 uM after 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4143735 | B | Inhibition of NAE in human Caco2 cells assessed as increase in phosphorylated NF-kappaB protein levels at 0.1 to 10 uM after 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4222558 | B | Inhibition of human NAE1-UBA3 E1 activating enzyme assessed as reduction in CUL3 neddylation by measuring inhibition of UBE2M thiol-ester formation at 50 uM up to 60 mins by pulse-chase assay | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
| 18. | ALA4271832 | B | Inhibition of recombinant human NAE assessed as reduction in Ubc12-NEDD8 conjugation level pretreated for 10 mins followed by Mg2+ and ATP addition measured after 60 mins by Western blot analysis | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 19. | ALA4271833 | B | Inhibition of NAE in human AGS cells assessed as reduction in Uba3-NEDD8 conjugation level after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA4271834 | B | Inhibition of NAE in human AGS cells assessed as reduction in Ubc12-NEDD8 conjugation level after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |
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