# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3855158 | B | Inhibition of N-terminal His-tagged recombinant Escherichia coli K-12 RNase T exonuclease activity expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL preincubated for 10 mins followed by [gamma-32P]ATP labelled 11-nucleotide ssDNA substrate addition for 40 mins by PAGE based autoradiography | Escherichia coli K-12 | 9 | ALA3853325 | assay format | Scientific Literature | |
2. | ALA3855161 | B | Inhibition of 5'-[gamma-32P]ATP-labelled 11-nucleotide ssDNA binding to recombinant Escherichia coli K-12 N-terminal His-tagged RNase T expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL at 1 mM preincubated for 10 mins followed by substrate addition measured after 20 mins by TBE gel electrophoresis mobility shift assay | Escherichia coli K-12 | 9 | ALA3853325 | assay format | Scientific Literature |