#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3862654	B	Increase in BMP2-induced Smad1/5/8 phosphorylation in mouse C2C12 cells assessed as ratio of phosphorylated Smad1/5/8 to Smad1/5/8 in nuclear fraction at 10 uM preincubated with cells followed by BMP2 addition for 4 hrs by Western blot analysis (Rvb = 1.2 No_unit)	Mus musculus	1		cell-based format	Scientific Literature
2.	ALA3862658	B	Increase in BMP2-induced Smad1/5/8 nuclear translocation in mouse C2C12 cells assessed as ratio of Smad1/5/8 to lamin B1 in nuclear fraction at 10 uM preincubated with cells followed by BMP2 addition for 4 hrs by Western blot analysis (Rvb = 1.5 No_unit)	Mus musculus	1		cell-based format	Scientific Literature
3.	ALA3862659	B	Increase in BMP2-induced Smad1/5/8 nuclear translocation in mouse C2C12 cells assessed as ratio of Smad1/5/8 to actin in cytosol fraction at 10 uM preincubated with cells followed by BMP2 addition for 4 hrs by Western blot analysis (Rvb = 0.4 No_unit)	Mus musculus	1		cell-based format	Scientific Literature
4.	ALA3862660	B	Activation of Smad1/5/8 in mouse C2C12 cells assessed as increase in BMP2-stimulated Runx2 mRNA expression at 10 uM preincubated with cells followed by BMP2 addition after 3 days by real-time PCR analysis	Mus musculus	1		cell-based format	Scientific Literature
5.	ALA3862661	B	Activation of Smad1/5/8 in mouse C2C12 cells assessed as increase in BMP2-stimulated OCL mRNA expression at 10 uM preincubated with cells followed by BMP2 addition after 3 days by real-time PCR analysis	Mus musculus	1		cell-based format	Scientific Literature
6.	ALA3862662	B	Activation of Smad1/5/8 in mouse C2C12 cells assessed as increase in BMP2-stimulated OPN mRNA expression at 10 uM preincubated with cells followed by BMP2 addition after 3 days by real-time PCR analysis	Mus musculus	1		cell-based format	Scientific Literature
7.	ALA4018247	B	Induction of Smad1/5/8 phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated Smad1/5/8 to actin beta protein level at 30 uM measured after 5 mins by Western blot analysis relative to control	Mus musculus	2		cell-based format	Scientific Literature
8.	ALA4018252	B	Induction of Smad1/5/8 phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated Smad1/5/8 to actin beta protein level at 30 uM measured after 15 mins by Western blot analysis relative to control	Mus musculus	2		cell-based format	Scientific Literature
9.	ALA4018253	B	Induction of Smad1/5/8 phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated Smad1/5/8 to actin beta protein level at 30 uM measured after 30 mins by Western blot analysis relative to control	Mus musculus	2		cell-based format	Scientific Literature