| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3859851 | B | Activation of recombinant human His-tagged AMPK alpha2 (2 to 552 residues)/beta1 (2 to 270 residues)/gamma2 (2 to 569 residues) expressed in baculovirus infected sf21 cells preincubated for 30 mins followed by biotinylated ACC-CREBp peptide substrate addition measured after 45 mins by HTRF assay | Homo sapiens | 1 | ALA3856285 | cell-based format | Scientific Literature | |
| 2. | ALA3859866 | B | Activation of recombinant human His-tagged AMPK alpha2 (2 to 552 residues)/beta1 (2 to 270 residues)/gamma2 (2 to 569 residues) expressed in baculovirus infected sf21 cells preincubated for 30 mins followed by biotinylated ACC-CREBp peptide substrate addition measured after 45 mins by HTRF assay relative to AMP | Homo sapiens | 1 | ALA3856285 | cell-based format | Scientific Literature | |
| 3. | ALA4026640 | B | Agonist activity at human recombinant phosphorylated AMPK complex 8 alpha2/beta1/gamma2 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins | Homo sapiens | 1 | ALA4024751 | cell-based format | Scientific Literature | |
| 4. | ALA4026651 | B | Agonist activity at human recombinant phosphorylated AMPK complex 8 alpha2/beta1/gamma2 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate at 2 nM to 50 uM preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins | Homo sapiens | 1 | ALA4024751 | cell-based format | Scientific Literature | |
| 5. | ALA4144388 | B | Activation of CaMKK2-phosphorylated AMPK8 alpha2/beta1/gamma2 (unknown origin) using fluorescein-labeled SAMS peptide as substrate pretreated for 30 mins in presence of AMP followed by substrate addition measured after 60 mins by fluorescence assay | Homo sapiens | 1 | ALA4138270 | protein complex format | Scientific Literature | |
| 6. | ALA4887235 | B | AMPK (A2/B1/G2) Invitrogen Lab (UK) kinase screen (BI) | Homo sapiens | 1 | ALA4800722 | protein complex format | Donated Chemical Probes - SGC Frankfurt |
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