| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3881384 | B | Competitive inhibition of TLR1/2 (unknown origin) expressed in baculovirus infected Sf9 insect cells in presence of rhodamine-labeled Pam3CSK4 by fluorescence anisotropic analysis | Homo sapiens | 2 | ALA3880440 | cell-based format | Patent Bioactivity Data | |
| 2. | ALA3881387 | B | Antagonist activity at TLR1/2 in primary microglial cells (unknown origin) assessed as reduction in Pam3CSK4-induced IL-1beta mRNA expression level after 2 hrs | Homo sapiens | 1 | ALA3880440 | cell-based format | Patent Bioactivity Data | |
| 3. | ALA4195476 | B | Antagonist activity at human TLR1/2 expressed in HEK293 cells assessed as decrease in Pam3CSK4-induced NF-kappaB activation-mediated SEAP production at 10'-9 to 10'-5 M after 24 hrs by Quanti-blue-based assay | Homo sapiens | 1 | ALA4190396 | cell-based format | Scientific Literature | |
| 4. | ALA4222357 | B | Antagonist activity at human TLR1/2 expressed in HEK-blue cells assessed as inhibition of Pam3CSK4-induced NF-kappaB activation-mediated SEAP production at 25 uM after 24 hrs by Quanti-blue-based assay | Homo sapiens | 1 | ALA4219143 | cell-based format | Scientific Literature | |
| 5. | ALA4222365 | B | Antagonist activity at human TLR1/2 expressed in HEK-blue cells assessed as inhibition of Pam3CSK4-induced IL-8 mRNA expression at 100 uM preincubated for 1 hr followed by Pam3CSK4 induction measured after 1 hr by qRT-PCR method relative to control | Homo sapiens | 1 | ALA4219143 | cell-based format | Scientific Literature | |
| 6. | ALA4235562 | B | Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells co-expressing pGL3-Elam.luc assessed as inhibition of Pam3CSK4-stimulated NF-kappaB activation at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 5 hrs by luciferase reporter gene assay relative to control | Homo sapiens | 9 | ALA4229513 | cell-based format | Scientific Literature | |
| 7. | ALA4235563 | B | Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells co-expressing pGL3-Elam.luc assessed as Pam3CSK4-stimulated NF-kappaB activation at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 5 hrs by luciferase reporter gene assay relative to control | Homo sapiens | 2 | ALA4229513 | cell-based format | Scientific Literature | |
| 8. | ALA4235564 | B | Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells co-expressing pGL3-Elam.luc assessed as inhibition of Pam3CSK4-stimulated NF-kappaB activation preincubated for 1 hr followed by Pam3CSK4 stimulation for 5 hrs by luciferase reporter gene assay | Homo sapiens | 2 | ALA4229513 | cell-based format | Scientific Literature | |
| 9. | ALA4235566 | B | Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated MAPK p38 phosphorylation at Thr180/Tyr182 residues at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 35 mins by immunoblot analysis | Homo sapiens | 2 | ALA4229513 | cell-based format | Scientific Literature | |
| 10. | ALA4235567 | B | Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated JNK phosphorylation at Thr183/Tyr185 residues at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 35 mins by immunoblot analysis | Homo sapiens | 2 | ALA4229513 | cell-based format | Scientific Literature | |
| 11. | ALA4235568 | B | Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated MAPK p38 phosphorylation at Thr180/Tyr182 residues at 25 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 35 mins by immunoblot analysis | Homo sapiens | 1 | ALA4229513 | cell-based format | Scientific Literature | |
| 12. | ALA4235569 | B | Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated JNK phosphorylation at Thr183/Tyr185 residues at 25 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 35 mins by immunoblot analysis | Homo sapiens | 1 | ALA4229513 | cell-based format | Scientific Literature | |
| 13. | ALA4235570 | B | Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated IkBalpha degradation at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 35 mins by immunoblot analysis | Homo sapiens | 2 | ALA4229513 | cell-based format | Scientific Literature | |
| 14. | ALA4235571 | B | Antagonist activity at TLR2/TLR1 in human THP1 cells assessed as inhibition of Pam3CSK4-stimulated non-canonical NF-kB pathway by measuring reduction in p52 level at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 2 hrs by immunoblot analysis | Homo sapiens | 2 | ALA4229513 | cell-based format | Scientific Literature | |
| 15. | ALA4235572 | B | Antagonist activity at TLR2/TLR1 in human PBMC assessed as inhibition of Pam3CSK4-stimulated TNFalpha production at 50 uM preincubated with cells followed by overnight Pam3CSK4 stimulation by ELISA relative to control | Homo sapiens | 2 | ALA4229513 | cell-based format | Scientific Literature | |
| 16. | ALA4235574 | B | Antagonist activity at TLR2/TLR1 in human PBMC assessed as inhibition of Pam3CSK4-stimulated IL-10 production by measuring IL-10 level at 100 uM preincubated with cells followed by overnight Pam3CSK4 stimulation by ELISA (Rvb = 794 pg/ml) | Homo sapiens | 1 | ALA4229513 | cell-based format | Scientific Literature | |
| 17. | ALA4235575 | B | Antagonist activity at TLR2/TLR1 in human PBMC assessed as inhibition of Pam3CSK4-stimulated IL-10 production at 100 uM preincubated with cells followed by overnight Pam3CSK4 stimulation by ELISA | Homo sapiens | 1 | ALA4229513 | cell-based format | Scientific Literature | |
| 18. | ALA4235585 | B | Antagonist activity at FLAG-tagged human TLR2/YFP-tagged TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated TLR1/TLR2 heterodimerization at 25 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 15 mins by immunoblot analysis | Homo sapiens | 2 | ALA4229513 | cell-based format | Scientific Literature | |
| 19. | ALA4346931 | B | Agonist activity at human TLR1/2 expressed in HEK-Blue cells assessed as activation of SEAP at 0.5 uM incubated for 24 hrs by quanti-blue SEAP assay in presence of Anti-hTLR2-IgA | Homo sapiens | 1 | ALA4346656 | cell-based format | Scientific Literature | |
| 20. | ALA4346936 | B | Agonist activity at human TLR1/2 expressed in HEK-Blue cells assessed as activation of SEAP at 0.5 uM incubated for 24 hrs by quanti-blue SEAP assay in presence of Anti-hTLR1-IgG | Homo sapiens | 1 | ALA4346656 | cell-based format | Scientific Literature |
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