#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2150440	B	Inhibition of TLR1/TLR2 in Pam3CSK4-stimulated mouse RAW264.7 cells at 0.6 uM after 24 hrs	Mus musculus	1		cell-based format	Scientific Literature
2.	ALA3881372	B	Antagonist activity at TLR1/2 in mouse RAW264.7 cells assessed as inhibition of Pam3CSK4-induced receptor activation by measuring NO production at 3 uM after 24 hrs	Mus musculus	10		cell-based format	Patent Bioactivity Data
3.	ALA3881373	B	Antagonist activity at TLR1/2 in mouse RAW264.7 cells assessed as inhibition of Pam3CSK4-induced receptor activation by measuring NO production after 24 hrs	Mus musculus	27		cell-based format	Patent Bioactivity Data
4.	ALA4235573	B	Antagonist activity at TLR2/TLR1 in mouse BMDM assessed as inhibition of Pam3CSK4-stimulated TNFalpha production by measuring TNFalpha level at 25 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 11 hrs by ELISA relative to control	Mus musculus	2		cell-based format	Scientific Literature
5.	ALA4235582	B	Antagonist activity at TLR2/TLR1 in mouse BMDM assessed as inhibition of Rhodamine-labeled Pam3CSK4-stimulated receptor internalization at 50 uM preincubated for 1 hr followed by Rhodamine-Pam3CSK4 stimulation for 20 mins by DAPI staining based phase contrast fluorescence microscopic method	Mus musculus	2		cell-based format	Scientific Literature
6.	ALA4235583	B	Antagonist activity at TLR2/TLR1 in mouse BMDM assessed as inhibition of Rhodamine-labeled Pam3CSK4-stimulated receptor internalization at 1 uM preincubated for 1 hr followed by Rhodamine-Pam3CSK4 stimulation for 20 mins by FACS analysis	Mus musculus	1		cell-based format	Scientific Literature
7.	ALA4235584	B	Antagonist activity at TLR2/TLR1 in mouse BMDM assessed as Rhodamine-labeled Pam3CSK4-stimulated receptor internalization at 50 uM preincubated for 1 hr followed by Rhodamine-Pam3CSK4 stimulation for 20 mins by FACS analysis relative to control	Mus musculus	2		cell-based format	Scientific Literature
8.	ALA4361579	B	Antagonist activity at TLR2/1 in mouse RAW264.7 cells assessed as reduction in PAM3CSK4-induced TNFalpha secretion at 20 to 80 uM after 24 hrs by ELISA	Mus musculus	1		cell-based format	Scientific Literature
9.	ALA4434017	B	Agonist activity at TLR2/1 in mouse BV2 cells assessed as induction of IL-6 production at 2 uM incubated for 24 hrs in presence of TLR2/1 inhibitor CU-CPT22 by ELISA	Mus musculus	1		cell-based format	Scientific Literature
10.	ALA4687759	B	Inhibition of Pam3CSK4-induced TLR1/TLR2 activation in mouse RAW 264.7 cells assessed as reduction in NO production at 3 uM incubated for 24 hrs by 2,3-diaminonaphthalene reagent based assay relative to control	Mus musculus	1		cell-based format	Scientific Literature
11.	ALA4687760	B	Inhibition of Pam3CSK4-induced TLR1/TLR2 activation in mouse RAW 264.7 cells assessed as reduction in NO production incubated for 24 hrs by 2,3-diaminonaphthalene reagent based assay	Mus musculus	1		cell-based format	Scientific Literature
12.	ALA5149150	B	Agonist activity at TLR1/TLR2 in wild type C57BL/6J mouse macrophages assessed as stimulation of TNF alpha release measured for 4 hrs by ELISA based assay	Mus musculus	94		cell-based format	Scientific Literature
13.	ALA5149151	B	Agonist activity at TLR1/TLR2 in wild type C57BL/6J mouse macrophages assessed as stimulation of TNF alpha release measured for 4 hrs by ELISA based assay relative to diprovocim-1	Mus musculus	78		cell-based format	Scientific Literature