#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4360951	Binding	Inhibition of TNAS binding to human sepiapterin reductase at 25 uM by 19F-NMR spectra analysis relative to control	Homo sapiens	2	ALA4359832	single protein format	Scientific Literature
2.	ALA4360952	Binding	Inhibition of TNAS binding to human sepiapterin reductase by 19F-NMR spectra analysis	Homo sapiens	65	ALA4359832	single protein format	Scientific Literature
3.	ALA4360958	Binding	Inhibition of human sepiapterin reductase using L-sepiapterin as substrate preincubated for 15 mins followed by substrate addition	Homo sapiens	1	ALA4359832	single protein format	Scientific Literature
4.	ALA3887419	Binding	Chromogenic assay: To screen for SPR inhibition, a biochemical assay based on LC/MS (and chromogenic) read-out has been developed. The LC/MS assay monitors the product formation (L-biopterin) and the chromogenic assay measures OD at 420 nm.N-methoxyacetyl serotonin was used as a reference compound (positive control). The IC50 measured using the screening conditions was 20-40 nM, which agrees with the literature (Smith et al., Journal of Biological Chemistry, 297:5601, 1992). The exemplary assay protocol uses the following conditions: SPR (6 nM); L-Sepiapterin (50 uM); NADPH (100 uM); Na-Phosphate buffer, pH 6.5 (100 mM); 82 uL assay volume; 60 minutes incubation with compounds (0.5% final concentration in DMSO) at 37° C. in Greiner uClear 384 well plates.The following experimental procedure was applied: (1) Add 2 uL compound (inhibitor) dilutions (20% DMSO) in Greiner uClear 384 well plates. (2) Add 40 uL enzyme/assay buffer.		12	ALA3886312	single protein format	BindingDB Database
5.	ALA3887420	Binding	LC/MS assay: To screen for SPR inhibition, a biochemical assay based on LC/MS (and chromogenic) read-out has been developed. The LC/MS assay monitors the product formation (L-biopterin) and the chromogenic assay measures OD at 420 nm.N-methoxyacetyl serotonin was used as a reference compound (positive control). The IC50 measured using the screening conditions was 20-40 nM, which agrees with the literature (Smith et al., Journal of Biological Chemistry, 297:5601, 1992).The exemplary assay protocol uses the following conditions: SPR (6 nM); L-Sepiapterin (50 uM); NADPH (100 uM); Na-Phosphate buffer, pH 6.5 (100 mM); 82 uL assay volume; 60 minutes incubation with compounds (0.5% final concentration in DMSO) at 37° C. in Greiner uClear 384 well plates.The following experimental procedure was applied: (1) Add 2 uL compound (inhibitor) dilutions (20% DMSO) in Greiner uClear 384 well plates. (2) Add 40 uL enzyme/assay buffer.		12	ALA3886312	single protein format	BindingDB Database
6.	ALA4482726	Binding	Inhibition of terbium-labeled SNAP-tagged human SPR in presence of sulfasalazine-labeled SNAP-tagged EGFP by homogenous TR-FRET assay	Homo sapiens	834	ALA4482318	single protein format	Patent Bioactivity Data
7.	ALA4482727	Binding	Inhibition of terbium-labeled SNAP-tagged human SPR in presence of sulfasalazine-labeled SNAP-tagged EGFP by TR-FRET assay	Homo sapiens	29	ALA4482318	single protein format	Patent Bioactivity Data
8.	ALA4482728	Binding	Inhibition of SPR in human SK-N-BE(2) cells assessed as reduction in BH4 levels incubated for 6 to 48 hrs by LCMS analysis	Homo sapiens	640	ALA4482318	cell-based format	Patent Bioactivity Data
9.	ALA4482729	Binding	Inhibition of SPR in human PBMC cells assessed as reduction in BH4 production incubated for 12 to 48 hrs by LCMS analysis	Homo sapiens	355	ALA4482318	cell-based format	Patent Bioactivity Data
10.	ALA4603112	Binding	Inhibition of sepiapterin reductase (unknown origin)	Homo sapiens	50	ALA4602609	single protein format	Scientific Literature