#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4419004	Functional	Agonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R8 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as increase in intracellular calcium level at 100 uM by FLIPR assay	Homo sapiens	1	ALA4418912	cell-based format	Patent Bioactivity Data
2.	ALA4419037	Functional	Antagonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R8 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as inhibition of chloramphenicol-induced increase in intracellular calcium level at 100 uM by FLIPR assay	Homo sapiens	2	ALA4418912	cell-based format	Patent Bioactivity Data
3.	ALA4419204	Functional	Agonist activity at human TAS2R8 expressed in HEK293T cells co-expressing Galpha15 assessed as increase in intracellular calcium level by Calcium-3 dye based fluorescence assay	Homo sapiens	1	ALA4419163	cell-based format	Patent Bioactivity Data
4.	ALA4419229	Functional	Agonist activity at human TAS2R8 expressed in HEK293T cells co-expressing Galpha15 assessed as increase in intracellular calcium level at 20 mM by Calcium-3 dye based fluorescence assay relative to control	Homo sapiens	3	ALA4419163	cell-based format	Patent Bioactivity Data
5.	ALA3888094	Binding	Fluorescence Polarization Assays: In another embodiment, Fluorescence Polarization ("FP") based assays may be used to detect and monitor ligand binding. Fluorescence polarization is a versatile laboratory technique for measuring equilibrium binding, nucleic acid hybridization, and enzymatic activity. Fluorescence polarization assays are homogeneous in that they do not require a separation step such as centrifugation, filtration, chromatography, precipitation, or electrophoresis. These assays are done in real time, directly in solution and do not require an immobilized phase. Polarization values can be measured repeatedly and after the addition of reagents since measuring the polarization is rapid and does not destroy the sample. Generally, this technique can be used to measure polarization values of fluorophores from low picomolar to micromolar levels.		307	ALA3886188	single protein format	BindingDB Database
6.	ALA3888095	Binding	HTS assay: To determine the effectiveness of an individual antagonist, taste tests were performed with a T2R8 specific agonist, the compound of interest and a reference bitter blocker. We have previously described a good hT2R8 antagonist that was proven to have taste effect. It was shown to reduce bitterness of coffee by itself and in combination with a Broad spectrum bitter blocker.		1	ALA3886188	single protein format	BindingDB Database
7.	ALA4346403	Functional	Antagonist activity at recombinant human TAS2R8 stably expressed in cells co-expressing Galpha16gust44 assessed as inhibition of andrographolide-induced intracellular calcium level at 10 uM measured for 100 secs by fluo-4 dye based FLIPR assay relative to control	Homo sapiens	1	ALA4342523	cell-based format	Scientific Literature
8.	ALA4346404	Functional	Antagonist activity at recombinant human TAS2R8 stably expressed in cells co-expressing Galpha16gust44 assessed as inhibition of andrographolide-induced intracellular calcium level measured for 100 secs by fluo-4 dye based FLIPR assay	Homo sapiens	56	ALA4342523	cell-based format	Scientific Literature
9.	ALA4346419	Functional	Antagonist activity at recombinant human TAS2R8 assessed as inhibition of andrographolide-induced intracellular calcium level at 1 uM preincubated for 60 secs followed by andrographolide addition using Fura-2AM dye by cell-based fluorescent microscopic analysis	Homo sapiens	1	ALA4342523	cell-based format	Scientific Literature
10.	ALA4346420	Functional	Reversible antagonist activity at recombinant human TAS2R8 assessed as recovery of suppression of andrographolide-induced intracellular calcium level at 1 uM preincubated for 60 secs followed by andrographolide addition measured after 5 mins washout period using Fura-2AM dye by cell-based fluorescent microscopic analysis	Homo sapiens	1	ALA4342523	cell-based format	Scientific Literature
11.	ALA4346421	Functional	Competitive antagonist activity at recombinant human TAS2R8 assessed as inhibition of andrographolide-induced intracellular calcium level at 3 to 3000 nM preincubated for 60 secs followed by varying levels of andrographolide addition using Fura-2AM dye by cell-based fluorescent microscopic analysis	Homo sapiens	1	ALA4342523	cell-based format	Scientific Literature
12.	ALA4346427	Functional	Antagonist activity at recombinant human TAS2R8 transiently expressed in HEK293T co-expressing Galpha16gust44 assessed as inhibition of andrographolide-induced intracellular calcium level by measuring remaining activity at 25 uM by fluo-4AM dye based FLIPR assay relative to control	Homo sapiens	2	ALA4342523	cell-based format	Scientific Literature
13.	ALA4421741	Functional	Agonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R8 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as effect on intracellular calcium level at 100 uM by FLIPR assay	Homo sapiens	1	ALA4421717	cell-based format	Patent Bioactivity Data
14.	ALA4421770	Functional	Activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R8 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as effect on chloramphenicol-induced intracellular calcium level at 100 uM by FLIPR assay	Homo sapiens	2	ALA4421717	cell-based format	Patent Bioactivity Data