#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888551	B	Enzyme Inhibition Assay : Plasmepsin-2 (PM-2; Plm II) and Plasmepsin (PM-4; Plm IV) expression and purification was performed following the published protocols (Istvan E S and Goldberg D E, 2005). The final purified protein was activated by diluting the protein to 0.3 mg/mL in activating buffer (0.1 M citrate pH 4.5, 0.1% Tween-20, 50 mM dithiothreitol) and incubated at room temperature for 40 min, then the activated enzyme was diluted in assay buffer (50 mM sodium acetate pH 4.7, 0.01% Tween-20). The enzymatic inhibition reaction was performed in 384 well plates with a total volume of 20 μl. 10 μl of diluted PM-2 or PM-4 enzyme was added to the 384-well plate except blank wells (blank wells add 10 μL of assay buffer) and 20 nL of serials of diluted 1000× compounds were added to the wells with 520 Echo® Liquid Handling System (Labcyte Inc.). 10 μL PM-2 peptide substrate (AnaSpec, Cat#, 62050) with assay buffer was then added to final concentration of 20 μM to start the reaction.		11	ALA3886201	assay format	BindingDB Database
2.	ALA4125626	B	Inhibition of Plasmodium falciparum Plm4 at 10 uM using synthetic substrate pretreated for 5 mins followed by substrate addition measured immediately by spectrophotometric method relative to control	Plasmodium falciparum	3	ALA4118288	single protein format	Scientific Literature
3.	ALA4125628	B	Inhibition of Plasmodium falciparum Plm4 using synthetic substrate pretreated for 5 mins followed by substrate addition measured immediately by spectrophotometric method	Plasmodium falciparum	3	ALA4118288	single protein format	Scientific Literature
4.	ALA4223546	B	Inhibition of Plasmodium falciparum plasmepsin 4 using DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS as substrate preincubated for 30 mins followed by substrate addition measured every 1 min within 8 to 15 mins by FRET assay	Plasmodium falciparum	21	ALA4219216	single protein format	Scientific Literature
5.	ALA4331647	B	Inhibition of Plasmodium falciparum plasmepsin 4 using DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS as substrate preincubated for 30 mins followed by substrate addition and measured every min for 8 to 15 mins by FRET assay	Plasmodium falciparum	28	ALA4330085	single protein format	Scientific Literature
6.	ALA4355295	B	Inhibition of Plasmodium falciparum plasmepsin 4 by FRET assay	Plasmodium falciparum	5	ALA4354799	single protein format	Scientific Literature
7.	ALA4355297	B	Inhibition of Plasmodium falciparum plasmepsin 4 using fluorescently labeled peptide substrate by fluorescence method	Plasmodium falciparum	13	ALA4354799	single protein format	Scientific Literature
8.	ALA4357661	B	Inhibition of recombinant Plasmodium falciparum Plasmepsin 4	Plasmodium falciparum	15	ALA4354841	single protein format	Scientific Literature
9.	ALA4392032	B	Inhibition of Plasmodium falciparum plasmepsin 4 using EDANS-CO-CH2-CH2-CO-ALERMFLSFP-Dap(DABCYL)OH as substrate measured after 60 mins by fluorescence assay	Plasmodium falciparum	10	ALA4390670	single protein format	Scientific Literature