| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4135757 | B | Inhibition of IDH2 R172K mutant (unknown origin) expressed in human HCT116 cells assessed as reduction in 2-HG level | Homo sapiens | 1 | ALA4130531 | cell-based format | Scientific Literature | |
| 2. | ALA4135758 | B | Inhibition of IDH2 R140Q mutant (unknown origin) expressed in human HCT116 cells assessed as reduction in 2-HG level | Homo sapiens | 1 | ALA4130531 | cell-based format | Scientific Literature | |
| 3. | ALA4180682 | B | Inhibition of IDH2 R140Q mutant (unknown origin) using alpha-ketoglutarate as substrate pretreated for 60 mins followed by substrate addition measured after 60 mins by resazurin dye based fluorescence assay | Homo sapiens | 2 | ALA4177635 | single protein format | Scientific Literature | |
| 4. | ALA4256250 | B | Inhibition of wild type IDH2/IDH2 R140Q mutant heterodimer (unknown origin) after 16 hrs in presence of NADPH by diaphorase/resazurin coupled fluorescence assay | Homo sapiens | 1 | ALA4251730 | single protein format | Scientific Literature | |
| 5. | ALA4256251 | B | Inhibition of wild type IDH2/IDH2 R172K mutant heterodimer (unknown origin) after 16 hrs in presence of NADPH by diaphorase/resazurin coupled fluorescence assay | Homo sapiens | 1 | ALA4251730 | single protein format | Scientific Literature | |
| 6. | ALA4257014 | B | Inhibition of IDH2 R140Q mutant homodimer (unknown origin) expressed in Sf9 cells pre-incubated for 1 hr before addition of alpha-KG followed by 16 hrs incubation in presence of NADPH, NADP, diaphorase and resazurin by fluorescence spectrometry based cofactor depletion assay | Homo sapiens | 256 | ALA4257005 | cell-based format | Patent Bioactivity Data | |
| 7. | ALA4257015 | B | Inhibition of IDH2 R140Q mutant homodimer (unknown origin) expressed in Sf9 cells pre-incubated for 6 hrs before addition of alpha-KG followed by 60 mins incubation in presence of NADPH, NADP, diaphorase and resazurin by fluorescence spectrometry based cofactor depletion assay | Homo sapiens | 32 | ALA4257005 | cell-based format | Patent Bioactivity Data | |
| 8. | ALA4309349 | B | Inhibition of wild type N-terminal His-tagged IDH2 (unknown origin) expressed in Escherichia coli using alpha-KG as substrate assessed as decrease in D-2-HG production after 90 mins by quadrupole mass spectrometry | Homo sapiens | 1 | ALA4308836 | assay format | Scientific Literature | |
| 9. | ALA4309350 | B | Inhibition of N-terminal His-tagged IDH2 R140Q mutant (unknown origin) expressed in Escherichia coli using alpha-KG as substrate assessed as decrease in D-2-HG production after 90 mins by quadrupole mass spectrometry | Homo sapiens | 1 | ALA4308836 | assay format | Scientific Literature | |
| 10. | ALA4309351 | B | Inhibition of N-terminal His-tagged IDH2 R172K mutant (unknown origin) expressed in Escherichia coli using alpha-KG as substrate assessed as decrease in D-2-HG production after 90 mins by quadrupole mass spectrometry | Homo sapiens | 1 | ALA4308836 | assay format | Scientific Literature | |
| 11. | ALA4309368 | B | Inhibition of wild type IDH2 (unknown origin) | Homo sapiens | 1 | ALA4308836 | single protein format | Scientific Literature | |
| 12. | ALA4309369 | B | Inhibition of IDH2 R172K mutant (unknown origin) using alpha-KG as substrate after 60 mins in presence of NADPH by resazurin-based assay | Homo sapiens | 5 | ALA4308836 | single protein format | Scientific Literature | |
| 13. | ALA4309370 | B | Inhibition of IDH2 R140Q mutant (unknown origin) using alpha-KG as substrate after 60 mins in presence of NADPH by resazurin-based assay | Homo sapiens | 7 | ALA4308836 | single protein format | Scientific Literature | |
| 14. | ALA4309371 | B | Inhibition of IDH2 R140Q mutant in human U87MG cells assessed as reduction in 2-HG levels after 48 hrs by celltiter glo reagent-based assay | Homo sapiens | 5 | ALA4308836 | cell-based format | Scientific Literature | |
| 15. | ALA4309373 | B | Inhibition of IDH2 R140Q mutant (unknown origin) assessed as reduction in NADPH consumption using alpha-KG as substrate incubated for 60 mins by by diaphorase and resazurin dye based assay | Homo sapiens | 5 | ALA4308836 | single protein format | Scientific Literature | |
| 16. | ALA4309374 | B | Inhibition of IDH2 R172K mutant (40 to end residues) (unknown origin) using alpha-KG as substrate after 120 mins in presence of NADPH by resazurin-based assay | Homo sapiens | 4 | ALA4308836 | single protein format | Scientific Literature | |
| 17. | ALA4309375 | B | Inhibition of IDH2 R172K mutant (unknown origin) by NADPH depletion assay | Homo sapiens | 1 | ALA4308836 | single protein format | Scientific Literature | |
| 18. | ALA4309376 | B | Inhibition of IDH2 R140Q mutant (unknown origin) by NADPH depletion assay | Homo sapiens | 2 | ALA4308836 | single protein format | Scientific Literature | |
| 19. | ALA4309381 | B | Inhibition of IDH2 R172K mutant (unknown origin) assessed as reduction in 2-HG levels after 90 mins in presence of NADPH by resazurin-based assay | Homo sapiens | 1 | ALA4308836 | single protein format | Scientific Literature | |
| 20. | ALA4309382 | B | Inhibition of IDH2 R140Q mutant (unknown origin) assessed as reduction in 2-HG levels after 90 mins in presence of NADPH by resazurin-based assay | Homo sapiens | 1 | ALA4308836 | single protein format | Scientific Literature |
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