# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4054004 | B | Inhibition of Pseudomonas aeruginosa 6His-tagged GIM-1 (Q19 to D250 residues) expressed in Escherichia coli BL21 Star(DE3) pLysS using nitrocefin as substrate preincubated for 5 mins followed by substrate addition by spectrophotometric analysis | Pseudomonas aeruginosa | 23 | assay format | Scientific Literature | ||
2. | ALA4054007 | B | Inhibition of Pseudomonas aeruginosa GIM-1 expressed in Escherichia coli SNO3 whole cells using nitrocefin as substrate at 250 uM preincubated for 20 mins followed by substrate addition measured every min up to 60 mins by spectrophotometric analysis relative to control | Pseudomonas aeruginosa | 21 | cell-based format | Scientific Literature | ||
3. | ALA4726661 | B | Inhibition of beta lactamase GIM-1 in Pseudomonas aeruginosa in presence of nitrocefin as reporter substrate measured after 5 mins by microplate spectrophotometry analysis | Pseudomonas aeruginosa | 14 | single protein format | Scientific Literature | ||
4. | ALA4726664 | B | Inhibition of Pseudomonas aeruginosa beta lactamase GIM-1 transfected in negative Escherichia coli SNO3 cells assessed as substrate hydrolysis using nitrocefin as substrate at 250 uM incubated for 20 mins measured every 1 mins for 3 hrs by cell-based microplate spectrophotometry analysis relative to control | Pseudomonas aeruginosa | 14 | cell-based format | Scientific Literature |