| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4020060 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by ITC method | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 2. | ALA4020061 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged FANCL RING fused human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by ITC method | Homo sapiens | 5 | assay format | Scientific Literature | ||
| 3. | ALA4020062 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged Ub fused human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli at 1.5 mM by ITC method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 4. | ALA4020063 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli in presence of EDTA by ITC method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 5. | ALA4020064 | B | Inhibition of N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli charging with ubiquitin after 10 mins in presence of E1 and ATP by coomassie staining based SDS PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 6. | ALA4020066 | B | Binding affinity to biotin labeled Ube2T C86K/K91R/K95R mutant (unknown origin) assessed as association and dissociation response at 2 uM by biolayer interferometry analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 7. | ALA4020067 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by DSF method | Homo sapiens | 6 | assay format | Scientific Literature | ||
| 8. | ALA4020068 | B | Binding affinity to human 15N-labelled C-terminal flexible tail lacking Ube2T (1 to 154 residues) (unknown origin) at 500 uM by NMR spectrocopy | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 9. | ALA4020069 | B | Reversible binding affinity to human 15N-labelled C-terminal flexible tail lacking Ube2T (1 to 154 residues) at 100 to 500 uM measured after overnight dialysis by NMR spectrocopy | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 10. | ALA4043713 | B | Inhibition of full length N-terminal 6xHis-Smt3 tagged human Ube2T expressed in Escherichia coli BL21(DE3) assessed as FANCD2 ubiquitination after 15 mins in presence of fluorescently-labeled Ub/recombinant human E1/ATP by fluorescence assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 11. | ALA4043714 | B | Binding affinity to full length recombinant N-terminal 6xHis-Smt3 tagged human Ube2T P73K mutant expressed in Escherichia coli BL21(DE3) by ITC | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 12. | ALA4043715 | B | Binding affinity to [1H-15N]-labeled N-terminal 6xHis-Smt3 tagged human Ube2T (1 to 154 residues) expressed in Escherichia coli BL21(DE3) by chemical shift perturbation assay | Homo sapiens | 6 | assay format | Scientific Literature | ||
| 13. | ALA4043716 | B | Binding affinity to full length N-terminal 6xHis-Smt3 tagged human Ube2T expressed in Escherichia coli BL21(DE3) by ITC | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA4043717 | B | Inhibition of full length N-terminal 6xHis-Smt3 tagged human Ube2T expressed in Escherichia coli BL21(DE3) assessed as FANCD2 ubiquitination at 2.5 mM after 15 mins in presence of fluorescently-labeled Ub/recombinant human E1/FANCL in 0.05% Tween20 containing buffer by fluorescence assay | Homo sapiens | 3 | assay format | Scientific Literature |
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