# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4034309 | B | Inhibition of DGKA (unknown origin) at 10 uM | Homo sapiens | 1 | ALA4033637 | single protein format | Scientific Literature | |
2. | ALA4045669 | B | Inhibition of DGKA labeling at activation Loop (IDPVPNTHPLLVFVNPKSGGK) in human PBMC at 1 uM | Homo sapiens | 1 | ALA4043168 | single protein format | Scientific Literature | |
3. | ALA4045912 | B | Inhibition of DGKA labeling at activation Loop (IDPVPNTHPLLVFVNPKSGGK) in human PBMC at 0.1 uM | Homo sapiens | 1 | ALA4043168 | single protein format | Scientific Literature | |
4. | ALA4413386 | B | Inhibition of OST-tagged DGKalpha (unknown origin) expressed in MDCK cell homogenates assessed as residual activity at 100 uM using DAG as substrate measured after 5 mins in presence of [gamma-32P]ATP by TLC analysis relative to control | Homo sapiens | 17 | ALA4411310 | cell-based format | Scientific Literature | |
5. | ALA4413387 | B | Inhibition of OST-tagged DGKalpha (unknown origin) expressed in MDCK cell homogenates using DAG as substrate measured after 5 mins in presence of [gamma-32P]ATP by TLC analysis | Homo sapiens | 4 | ALA4411310 | cell-based format | Scientific Literature | |
6. | ALA4413388 | B | Inhibition of OST-tagged DGKalpha (unknown origin) expressed in MDCK cell homogenates at 100 uM using DAG as substrate measured after 5 mins in presence of [gamma-32P]ATP by TLC analysis relative to control | Homo sapiens | 3 | ALA4411310 | cell-based format | Scientific Literature | |
7. | ALA4413393 | B | Inhibition of DGKalpha in OKT3-induced human Jurkat cells harboring SAP ShRNA assessed as restoration of restimulation-induced cell death at 1 uM measured after 24 hrs by trypan blue exclusion method | Homo sapiens | 4 | ALA4411310 | cell-based format | Scientific Literature | |
8. | ALA4413394 | B | Inhibition of DGKalpha in PHA-induced human Jurkat cells harboring SAP ShRNA assessed as restoration of restimulation-induced cell death at 1 uM measured after 24 hrs by trypan blue exclusion method | Homo sapiens | 4 | ALA4411310 | cell-based format | Scientific Literature | |
9. | ALA4413397 | B | Inhibition of DGKalpha in OKT3-induced human T cells harboring SAP SiRNA assessed as restoration of restimulation-induced cell death at 1 to 10 uM preincubated for 30 mins followed by OKT3 stimulation and measured 24 hrs post dose by propidium iodide staining based flow cytometry | Homo sapiens | 3 | ALA4411310 | cell-based format | Scientific Literature | |
10. | ALA4413398 | B | Inhibition of DGKalpha in OKT3-induced human T cells harboring SAP SiRNA assessed as restoration of restimulation-induced cell death at >=5 uM preincubated for 30 mins followed by OKT3 stimulation and measured 24 hrs post dose by propidium iodide staining based flow cytometry | Homo sapiens | 1 | ALA4411310 | cell-based format | Scientific Literature | |
11. | ALA4413400 | B | Inhibition of DGKalpha in OKT3-induced human T cells harboring SAP siRNA assessed as restoration of TCR-induced NR4A1 upregulation at 10 uM after 4 hrs by RT-PCR analysis | Homo sapiens | 2 | ALA4411310 | cell-based format | Scientific Literature | |
12. | ALA4413401 | B | Inhibition of DGKalpha in OKT3-induced human T cells harboring SAP siRNA assessed as restoration of TCR-induced NR4A3 upregulation at 10 uM after 4 hrs by RT-PCR analysis | Homo sapiens | 1 | ALA4411310 | cell-based format | Scientific Literature | |
13. | ALA4413403 | B | Inhibition of DGKalpha in human erythrocytes using OAG as substrate preincubated for 1 min followed by OAG addition and measured after 8 mins in presence of [gamma-32P]ATP | Homo sapiens | 6 | ALA4411310 | cell-based format | Scientific Literature | |
14. | ALA4419837 | B | Inhibition of FLAG-tagged DGKa (unknown origin) using lipids and [gamma-32P]-ATP incubated for 15 mins by scintillation counting method | Homo sapiens | 2 | ALA4419176 | single protein format | Patent Bioactivity Data | |
15. | ALA4419854 | B | Inhibition of DGKa in human U251 cells transfected with mTOR promoter assessed as decrease in mTOR promoter activity at 20 uM incubated for 36 to 48 hrs by luciferase reporter gene assay | Homo sapiens | 1 | ALA4419176 | cell-based format | Patent Bioactivity Data | |
16. | ALA4419855 | B | Inhibition of DGKa in human JWL578 cells transfected with mTOR promoter assessed as decrease in mTOR promoter activity at 20 uM incubated for 36 to 48 hrs by luciferase reporter gene assay | Homo sapiens | 1 | ALA4419176 | cell-based format | Patent Bioactivity Data | |
17. | ALA4419856 | B | Inhibition of DGKa in human U251 cells transfected with HIF1a promoter assessed as decrease in HIF1a promoter activity at 20 uM incubated for 36 to 48 hrs by luciferase reporter gene assay | Homo sapiens | 1 | ALA4419176 | cell-based format | Patent Bioactivity Data | |
18. | ALA4419857 | B | Inhibition of DGKa in human U251 cells assessed as HSP60 protein level at 15 uM incubated for 24 hrs by dot-blot proteomics assay relative to baseline | Homo sapiens | 1 | ALA4419176 | cell-based format | Patent Bioactivity Data | |
19. | ALA4419858 | B | Inhibition of DGKa in human U251 cells assessed as reduction in P70 S6K phosphorylation at T389/T421 residues at 15 uM incubated for 24 hrs by dot-blot proteomics assay relative to baseline | Homo sapiens | 1 | ALA4419176 | cell-based format | Patent Bioactivity Data | |
20. | ALA4419859 | B | Inhibition of DGKa in human U251 cells assessed as reduction in p53 phosphorylation at S15 residues at 15 uM incubated for 24 hrs by dot-blot proteomics assay relative to baseline | Homo sapiens | 1 | ALA4419176 | cell-based format | Patent Bioactivity Data |